Proteomic and toxinological characterization of the venom of the South African Ringhals cobra Hemachatus haemachatus

Sánchez, Andrés; Herrera, María; Villalta, Mauren; Solano, Daniela; Segura, Álvaro; Lomonte, Bruno; Gutiérrez, José Marı́a; León, Guillermo; Vargas, Mariángela

doi:10.1016/j.jprot.2018.04.007

Cited by 25 publications

(26 citation statements)

References 54 publications

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“…In this study, N. ashei venom was able to induce necrosis in the mouse model ( Table 1 ). Our findings concur with those of previous studies ( Petras et al, 2011 ), ( Otieno et al, 2021 ), ( Kandiwa et al, 2018 ), ( S á nchez et al, 2018 ), where several African spitting cobras were found to induce dermonecrosis. Cytotoxins are thought to provoke cell damage and necrosis in vitro and in vivo respectively, a function which is central to localized necrotic effect ( Ownby et al, 1993 ), ( Fletcher and Lizzo, 1987 ).…”

Section: Discussionsupporting

confidence: 94%

Evaluation of lethality and cytotoxic effects induced by Naja ashei (large brown spitting cobra) venom and the envenomation-neutralizing efficacy of selected commercial antivenoms in Kenya

et al. 2022

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Section: Discussionsupporting

confidence: 94%

Evaluation of lethality and cytotoxic effects induced by Naja ashei (large brown spitting cobra) venom and the envenomation-neutralizing efficacy of selected commercial antivenoms in Kenya

et al. 2022

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“…The pharmacological effect of the SVMPs in cobras has not been fully understood yet [ 13 ]. Few cobra SVMPs were biologically studied.…”

Section: Resultsmentioning

confidence: 99%

Proteomic characterization of Naja mandalayensis venom

Beraldo

Coelho

Sciani

et al. 2021

J. Venom. Anim. Toxins incl. Trop. Dis

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Background: Naja mandalayensis is a spitting cobra from Myanmar. To the best of our knowledge, no studies on this venom composition have been conducted so far. On the other hand, few envenomation descriptions state that it elicits mainly local inflammation in the victims' eyes, the preferred target of this spiting cobra. Symptoms would typically include burning and painful sensation, conjunctivitis, edema and temporary loss of vision. Methods: We have performed a liquid-chromatography (C18-RP-HPLC) mass spectrometry (ESI-IT-TOF/MS) based approach in order to biochemically characterize N. mandalayensis venom. Results: A wide variety of three-finger toxins (cardiotoxins) and metallopeptidases were detected. Less abundant, but still representative, were cysteine-rich secretory proteins, L-amino-acid oxidases, phospholipases A 2 , venom 5'-nucleotidase and a serine peptidase inhibitor. Other proteins were present, but were detected in a relatively small concentration. Conclusion:The present study set the basis for a better comprehension of the envenomation from a molecular perspective and, by increasing the interest and information available for this species, allows future venom comparisons among cobras and their diverse venom proteins.

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“…Also, the similarities in the inhibition among the three Naja snakes is supported by Casassola et al [ 30 ] who observed that similar biochemical, antigenic and toxinological characteristics are shared by snake venoms that belong to the same genera. Similarly, the venom of Hemachatus haemachatus , an African spitting cobra-like species found predominantly in Southern Africa was reported to contain 63.3% 3FTx proteins [ 31 ], although it is closely related to the Naja genus phylogenetically.…”

Section: Discussionmentioning

confidence: 99%

Development of an Inhibition Enzyme-Linked Immunosorbent Assay (ELISA) Prototype for Detecting Cytotoxic Three-Finger Toxins (3FTxs) in African Spitting Cobra Venoms

et al. 2022

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The administration of toxin-specific therapy in snake envenoming is predicated on improved diagnostic techniques capable of detecting specific venom toxins. Various serological tests have been used in detecting snakebite envenoming. Comparatively, enzyme-linked immunosorbent assay (ELISA) has been shown to offer a wider practical application. We report an inhibition ELISA for detecting three-finger toxin (3FTx) proteins in venoms of African spitting cobras. The optimized assay detected 3FTxs in N. ashei (including other Naja sp.) venoms, spiked samples, and venom-challenged mice samples. In venoms of Naja sp., the assay showed inhibition, implying the detection of 3FTxs, but showed little or no inhibition in non-Naja sp. In mice-spiked samples, one-way ANOVA results showed that the observed inhibition was not statistically significant between spiked samples and negative control (p-value = 0.164). Similarly, the observed differences in inhibition between venom-challenged and negative control samples were not statistically significant (p-value = 0.9109). At an LOD of 0.01 µg/mL, the assay was able to confirm the presence of 3FTxs in the samples. Our results show a proof of concept for the use of an inhibition ELISA model as a tool for detecting 3FTxs in the venoms of African spitting cobra snakes.

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Proteomic and toxinological characterization of the venom of the South African Ringhals cobra Hemachatus haemachatus

Cited by 25 publications

References 54 publications

Evaluation of lethality and cytotoxic effects induced by Naja ashei (large brown spitting cobra) venom and the envenomation-neutralizing efficacy of selected commercial antivenoms in Kenya

Evaluation of lethality and cytotoxic effects induced by Naja ashei (large brown spitting cobra) venom and the envenomation-neutralizing efficacy of selected commercial antivenoms in Kenya

Proteomic characterization of Naja mandalayensis venom

Development of an Inhibition Enzyme-Linked Immunosorbent Assay (ELISA) Prototype for Detecting Cytotoxic Three-Finger Toxins (3FTxs) in African Spitting Cobra Venoms

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