Proteomic characterization and comparison of milk fat globule membrane proteins collected from two ethnic groups of women in China using DIA technique

Wang, Cuina; Zhao, Ru; Liu, Xinxin; Lu, Yingcong; Cheng, Jianjun; Jiang, Shilong; Guo, Mingruo

doi:10.1016/j.fbio.2022.102192

Cited by 4 publications

(3 citation statements)

References 29 publications

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“…As displayed in the Venn Diagram (Figure ), the protein types detected in both milk samples were 2513 (99%), with 9 (0.4%) detected only in the mother’s milk of the male infants’ group while 16 (0.6%) were observed in the milk of mothers with female infants. The technique applied in the present study substantially increased the quantity of MFGM proteins characterized in human milk in comparison with previous research, which were 312 and 1104 proteins, respectively. , In our earlier study, nearly 1596 MFGM proteins were identified using a data-independent acquisition (DIA) proteomic technique . Variations in the protein numbers may be due to the differences in milk sources, protein preparation, and detection techniques used.…”

Section: Resultsmentioning

confidence: 59%

“…Variations in the protein numbers may be due to the differences in milk sources, protein preparation, and detection techniques used. Compared with previous studies, − ,,,− some proteins related to immunity, digestion, and growth and development such as C8 β chain, tumor necrosis factor α-induced protein 8-like protein 2, epoxide hydrolase 1, and ephrin type-B receptor were first found in the human milk. A family of apolipoproteins including apolipoprotein A, B, C, D, E, and M with multiple beneficial functions were detected in the study, similar to a previous research …”

Section: Resultsmentioning

confidence: 87%

“…Herein, the MFGM protein profile of milk samples collected from mothers with female and male infants was investigated and compared using the latest novel 4D label-free proteomic technology. To make the results more specific, Korean women in northeast China were selected since there are obvious distinctions between people of ethnic groups . This study may provide useful information for a deep understanding of the nutritional composition of human milk.…”

Section: Introductionmentioning

confidence: 99%

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Insights from 4D Label-Free Proteomic Analysis into Variation of Milk Fat Globule Membrane Proteins of Human Milk Associated with Infant’s Gender

Wang

Zhao

et al. 2023

J. Agric. Food Chem.

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Milk fat globule membrane (MFGM) protein profiles of breast milk collected from women in northeast China with male or female babies were investigated using a four-dimensional (4D) label-free proteomic technique. Altogether, 2538 proteins were detected and quantified and 249 were differentially expressed, with 198 decreased proteins compared to the samples of mothers with female babies. Different proteins associated with infant’s gender were principally located in nuclear. The differentially expressed proteins were mainly involved in gene ontology (GO) functions of the cellular process, binding, and cell and found to be distributed in lipid-related biological processes and molecular functions to a large extent. The pathway of neurodegeneration-multiple disease ranked top for the altered proteins. The screened proteins were observed to contain some proteins related to typical functions of immunity, lipid metabolism, digestion, and growth and development. 114 proteins formed a relatively compact network (269 interactions) and dolichyl-diphospho-oligosaccharide–protein glycosyltransferase subunit 2 interacted the most with other proteins as the hub protein. MFGM proteins of breast milk were affected by the sex of offspring, and these findings may provide useful information for reasonable adjustments of infant formula powder specifically for boys or girls in the market.

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Section: Resultsmentioning

confidence: 59%