Proteomic characterization and comparison of milk fat globule membrane proteins of Saanen goat milk from 3 habitats in China using SWATH-MS technique

Wang, Cuina; Zhao, Ru; Zhao, Zixuan; Liu, Ning; Cheng, Jianjun; Guo, Mingruo

doi:10.3168/jds.2022-22393

Cited by 7 publications

(6 citation statements)

References 30 publications

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“…Table shows the major differential proteins such as casein, α-lactalbumin, β-lactoglobulin, and MFGM proteins. MFGM proteins such as XOR, perilipin, MUC1, and fatty acid-binding protein were all in high abundance, consistent with previous studies . Moreover, the highest relative abundance value was observed for GLYCAM1, which is a hormonally regulated glycoprotein and part of the milk mucin complex.…”

Section: Resultssupporting

confidence: 90%

“…Pathways with the highest three enrichment factors of proteins were complement and coagulation cascades (0.1), aminoacyl-tRNA biosynthesis (0.09), and the phospholipase D signaling pathway (0.08). Wang et al 33 also found complement and coagulation cascades and ribosome pathways in both MFGM proteins of Saanen breed caprine milk from three habitats in China. The complement system is a proteolytic cascade within the blood plasma and serves as a mediator of innate immunity, a nonspecific defense mechanism against pathogens.…”

Section: Abundance Of Identified Proteinsmentioning

confidence: 93%

“…MFGM proteins such as XOR, perilipin, MUC1, and fatty acid-binding protein were all in high abundance, consistent with previous studies. 33 Moreover, the highest relative abundance value was observed for GLYCAM1, which is a hormonally regulated glycoprotein and part of the milk mucin complex. The GLYCAM1 in the mammary gland showed different carbohydrate modifications in comparison to the endothelial form.…”

Section: Abundance Of Identified Proteinsmentioning

confidence: 99%

“…BTN3A1 is an important molecule in the activation of a subpopulation of T cells with innate properties that recognize phosphorylated nonprotein metabolites, which in turn enhance antitumor immune responses. 35 However, Wang et al 33 showed that lactadherin was the highest among MFGM proteins extracted from unpasteurized caprine milk, which may be due to differences in both analytical techniques and sample treatment procedures.…”

Section: Abundance Of Identified Proteinsmentioning

confidence: 99%

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Proteomic Comparisons of Caprine Milk Whole Cream Buttermilk Whey and Cheese Whey Cream Buttermilk

Song,

Lu,

Hou

et al. 2023

J. Agric. Food Chem.

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Buttermilk, a potential material used to produce milk fat globule membrane (MFGM), is obtained as a byproduct of butter making from milk whole cream and cheese whey cream. This study investigated the effects of rennet and acid coagulation on the protein profiles of buttermilk rennet-coagulated whey (BRW) and buttermilk acid-coagulated whey (BAW). They were compared to those of whey cream buttermilk (WCB). Rennet coagulation was more efficient in removing casein, while retaining more IgG and lactoferrin than acid coagulation. BRW had more MFGM than BAW. Butyrophilin, xanthine dehydrogenase, and mucin1 were significantly higher (P < 0.05) in BRW, while fatty acid-binding protein 3 was enriched in BAW. KEGG analysis showed that complement and coagulation cascades had the greatest differences, and the abundance of proteins involved in this signaling pathway in BRW and BAW was higher, suggesting their potential anticoagulant and anti-inflammatory activity. BAW had higher apolipoprotein A4 and transcobalamin 2, which are essential carriers for transporting long-chain fatty acids and vitamin B12 from the intestine to the blood. Therefore, BAW intake might improve lipids and vitamin B12 absorption. This study can help deepen the understanding of protein composition of MFGM-enriched whey and facilitate the production of MFGM proteins for infants and old-aged populations.

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Section: Resultssupporting

confidence: 90%

Section: Abundance Of Identified Proteinsmentioning

confidence: 93%

Section: Abundance Of Identified Proteinsmentioning

confidence: 99%

Section: Abundance Of Identified Proteinsmentioning

confidence: 99%

See 2 more Smart Citations

Proteomic Comparisons of Caprine Milk Whole Cream Buttermilk Whey and Cheese Whey Cream Buttermilk

Song,

Lu,

Hou

et al. 2023

J. Agric. Food Chem.

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“…To better understand the pathways of milk protein synthesis, proteomic techniques have been used to investigate the functional proteins in animal tissues [12][13][14]. Although standard (macro)proteomic application is suitable for large samples with protein losses measured in micrograms or milligrams, it is not sensitive enough for small numbers of cell samples.…”

Section: Introductionmentioning

confidence: 99%

Microproteomic-Based Analysis of the Goat Milk Protein Synthesis Network and Casein Production Evaluation

Chen,

Taniguchi,

Bagnicka

2024

Foods

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Goat milk has been consumed by humans since ancient times and is highly nutritious. Its quality is mainly determined by its casein content. Milk protein synthesis is controlled by a complex network with many signal pathways. Therefore, the aim of our study is to clearly depict the signal pathways involved in milk protein synthesis in goat mammary epithelial cells (GMECs) using state-of-the-art microproteomic techniques and to identify the key genes involved in the signal pathway. The microproteomic analysis identified more than 2253 proteins, with 323 pathways annotated from the identified proteins. Knockdown of IRS1 expression significantly influenced goat casein composition (α, β, and κ); therefore, this study also examined the insulin receptor substrate 1 (IRS1) gene more closely. A total of 12 differential expression proteins (DEPs) were characterized as upregulated or downregulated in the IRS1-silenced sample compared to the negative control. The enrichment and signal pathways of these DEPs in GMECs were identified using GO annotation and KEGG, as well as KOG analysis. Our findings expand our understanding of the functional genes involved in milk protein synthesis in goats, paving the way for new approaches for modifying casein content for the dairy goat industry and milk product development.

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Proteins and peptides: proteomics approaches for food authentication and allergen profiling

Carrera,

Abril,

Pazos

et al. 2024

Current Opinion in Food Science

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Proteomic characterization and comparison of milk fat globule membrane proteins of Saanen goat milk from 3 habitats in China using SWATH-MS technique

Cited by 7 publications

References 30 publications

Proteomic Comparisons of Caprine Milk Whole Cream Buttermilk Whey and Cheese Whey Cream Buttermilk

Proteomic Comparisons of Caprine Milk Whole Cream Buttermilk Whey and Cheese Whey Cream Buttermilk

Microproteomic-Based Analysis of the Goat Milk Protein Synthesis Network and Casein Production Evaluation

Proteins and peptides: proteomics approaches for food authentication and allergen profiling

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