Proteomic comparison of Chelidonium majus L. latex in different phases of plant development

Nawrot, Robert; Lippmann, Rico; Matros, Andrea; Musidlak, Oskar; Nowicki, Grzegorz; Mock, Hans‐Peter

doi:10.1016/j.plaphy.2017.01.010

Cited by 19 publications

(14 citation statements)

References 64 publications

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“…The composition of proteins in C. majus milky sap was studied by Nawrot et al ( 2007a , b , 2014 , 2017a , b ). The compounds were pre-separated using two-dimensional gel electrophoresis and further, HPLC on BEH C18 column (100 mm × 100 μm, 1.7 μm particle diameter) (Nawrot et al, 2017b ) or nano-HPLC on 75 μm analytical column (Nawrot et al, 2007a , 2014 , 2017a ) and gradient elution with mobile phase containing 0.1% (v/v) formic acid in water and in acetonitrile were performed. Proteins were identified by tandem mass spectrometry (MS/MS).…”

Section: Methods For Analysis Of Active Components From Chementioning

confidence: 99%

“…In a series of papers, Nawrot et al ( 2007a , b , 2008 , 2013 , 2014 , 2016 , 2017a , b ) described a number of proteins from root and leaf latex. Proteomic analysis using LC-ESI-MS/MS system revealed the presence of three categories of proteins according to their functions: proteins involved in disease and defenses responses (i.e., superoxide dismutases, lactoylglutathione lyases), nucleic acid binding proteins (i.e., glycine-rich proteins, nucleic acid binding, DNA-binding, or RNA-binding proteins), and these that are involved in general metabolism (acyl-CoA binding protein, malate dehydrogenase, flavodoxin-like quinone reductase, ubiquitin, polyubiquitin, serine/threonine protein kinases, rubber elongation factor).…”

Section: Phytochemistrymentioning

confidence: 99%

“…Their contribution to the traditional use of C. majus as antiviral and antimicrobial remedy has to be further explored and may, in combination with highly active alkaloids, render unique synergistic effects providing a multifaceted tool for combat against troublesome infections. In living plants, these proteins are probably also serving as a chemical defense against pathogens (Nawrot et al, 2007a , b , 2014 , 2017a , b ). Protein-bound polysaccharide (CM-AIa) bearing immunomodulatory and cytotoxic activity was isolated by Song et al ( 2002 ).…”

Section: Phytochemistrymentioning

confidence: 99%

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Greater Celandine's Ups and Downs−21 Centuries of Medicinal Uses of Chelidonium majus From the Viewpoint of Today's Pharmacology

et al. 2018

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As antique as Dioscorides era are the first records on using Chelidonium as a remedy to several sicknesses. Inspired by the “signatura rerum” principle and an apparent ancient folk tradition, various indications were given, such as anti-jaundice and cholagogue, pain-relieving, and quite often mentioned—ophthalmological problems. Central and Eastern European folk medicine has always been using this herb extensively. In this region, the plant is known under many unique vernacular names, especially in Slavonic languages, associated or not with old Greek relation to “chelidon”—the swallow. Typically for Papaveroidae subfamily, yellow-colored latex is produced in abundance and leaks intensely upon injury. Major pharmacologically relevant components, most of which were first isolated over a century ago, are isoquinoline alkaloids—berberine, chelerythrine, chelidonine, coptisine, sanguinarine. Modern pharmacology took interest in this herb but it has not ended up in gaining an officially approved and evidence-based herbal medicine status. On the contrary, the number of relevant studies and publications tended to drop. Recently, some controversial reports and sometimes insufficiently proven studies appeared, suggesting anticancer properties. Anticancer potential was in line with anecdotical knowledge spread in East European countries, however, in the absence of directly-acting cytostatic compounds, some other mechanisms might be involved. Other properties that could boost the interest in this herb are antimicrobial and antiviral activities. Being a common synanthropic weed or ruderal plant, C. majus spreads in all temperate Eurasia and acclimates well to North America. Little is known about the natural variation of bioactive metabolites, including several aforementioned isoquinoline alkaloids. In this review, we put together older and recent literature data on phytochemistry, pharmacology, and clinical studies on C. majus aiming at a critical evaluation of state-of-the-art from the viewpoint of historical and folk indications. The controversies around this herb, the safety and drug quality issues and a prospective role in phytotherapy are discussed as well.

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Section: Methods For Analysis Of Active Components From Chementioning

confidence: 99%

Section: Phytochemistrymentioning

confidence: 99%

Section: Phytochemistrymentioning

confidence: 99%

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Greater Celandine's Ups and Downs−21 Centuries of Medicinal Uses of Chelidonium majus From the Viewpoint of Today's Pharmacology

et al. 2018

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“…In Romania, it represents a very handy cure, the plant being ubiquitous on almost all pastures. C. majus performed better after the workout (41.86 ± 22.47 in leisure and 48.1 ± 6.82 in endurance horses), probably due to its complex, protein-rich latex structure (Nawrot et al, 2017).…”

Section: Resultsmentioning

confidence: 96%

“…The medicinal plants that represented the subject of the present study were chosen due to their high prevalence, thus the high likeliness to be ingested by the horses on the pasture. Nevertheless, researches indicated immunological effects for all including antiinflammatory effects (C. bursa pastoris, C. majus) (Choi et al, 2014;Nawrot et al, 2017), inhibition of activated lymphocyte proliferation (E. palustre, V. tricolor ) (Gründemann et al, 2014;Hellinger et al, 2014), anticomplementary activity (S. officinale) (Staiger, 2012), modulation of antibody levels (A. sativa, T. officinale) (Yoon, 2008;Chatuevedi et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

Original article Interdependence of productive effort and in vitro vegetal extract treatment on specific cell-mediated immunity in horses

Spînu¹,

Páll²,

Niculae³

et al. 2018

Ann. Phytomed

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The research aimed at identifying and rendering alcoholic extracts of various plants based on their in vitro potential in stimulating cell mediated immunity and in alleviating stress effects subsequent to workout type in horses.Experimental horses were selected from different working environments: (a) draft, agricultural works -n = 16, average age 8 years, (b) inconstant effort, leisure, n = 15, average age 7.5 years and (c) constant training, endurance, average age 3.5 years. Blood samples were collected in heparinized vials (50 UI/ml) before and after the workout. Alcoholic extracts (2 µl/well, duplicate) of Taraxacum officinale (L) Weber, Symphytum officinale L., Equisetum palustre L., Viola tricolor L., Avena sativa L., Capsella bursa pastoris (L) Medik., Hypericum perforatum L., Chelidonium majus L. were investigated by blast transformation test and statistically interpreted (Student's t test).In vitro responses were the highest to all tested extracts in endurance horses and the lowest in draft horses (p<0.01-0.001). C. bursa pastoris. was the most efficient in draft and endurance categories before the workout (draft 45.13 ± 8.05, in leisure 48.09 ± 24.13 and 53.44 ± 9.32 in endurance horses), but not after the workout, where C. majus performed better (41.86 ± 22.47 in leisure and 48.1 ± 6.82 in endurance horses), probably due to its complex, protein-rich latex structure. The effects of plant extracts depended on the type of effort and the level of constant training rather than on the taxonomy of the plant.

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Chelidonium majus L.

Göğer

2022

Novel Drug Targets With Traditional Herbal Medicines

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Proteomic comparison of Chelidonium majus L. latex in different phases of plant development

Cited by 19 publications

References 64 publications

Greater Celandine's Ups and Downs−21 Centuries of Medicinal Uses of Chelidonium majus From the Viewpoint of Today's Pharmacology

Greater Celandine's Ups and Downs−21 Centuries of Medicinal Uses of Chelidonium majus From the Viewpoint of Today's Pharmacology

Original article Interdependence of productive effort and in vitro vegetal extract treatment on specific cell-mediated immunity in horses

Chelidonium majus L.

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