Proteomic dissection of plant development

Hochholdinger, Frank; Sauer, M.R.; Dembinsky, Diana; Hoecker, Nadine; Muthreich, Nils; Saleem, Muhammad; Liu, Yan

doi:10.1002/pmic.200500851

Cited by 48 publications

(29 citation statements)

References 94 publications

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“…The use of proteomics in plant biology research has increased significantly over the last few years with an improvement in both quality and quantitative analysis, inaugurating a new phase of "Second Generation Plant Proteomics" (Jorrín et al, 2009). This growing interest in plant proteomics has continually produced a large number of developmental studies on plant cell division, elongation, differentiation, and formation of various organs using various proteomics approaches (Hochholdinger et al, 2006;Takàč et al, 2011, Miernyk et al, 2011. Most of the studies published in the plant field concern the proteome of Arabidopsis and rice.…”

Section: Proteomic Workflowmentioning

confidence: 99%

Proteomic Analyses of Cells Isolated by Laser Microdissection

Fiorilli¹,

Klink²,

Balestrini³

2012

Integrative Proteomics

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Section: Proteomic Workflowmentioning

confidence: 99%

Proteomic Analyses of Cells Isolated by Laser Microdissection

Fiorilli¹,

Klink²,

Balestrini³

2012

Integrative Proteomics

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“…High-throughput approaches such as proteomics, transcriptomics, and metabolomics have provided immense amounts of data, leading to a better understanding of biological processes (Hochholdinger et al, 2006). As the relationship between mRNA expression levels and protein abundance is poor, proteomics provides functional gene expression profiles during biological processes (Komatsu and Hossain, 2013).…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of flowers at two developmental stages in Thermopsis turcica (Fabaceae)

Yıldız¹,

Pehlivan²,

Terzi³

2017

Turk J Bot

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IntroductionPlant development is controlled by the interplay of multiple environmental cues and endogenous signals.High-throughput approaches such as proteomics, transcriptomics, and metabolomics have provided immense amounts of data, leading to a better understanding of biological processes (Hochholdinger et al., 2006). As the relationship between mRNA expression levels and protein abundance is poor, proteomics provides functional gene expression profiles during biological processes (Komatsu and Hossain, 2013). Flower development is controlled by a combination of genetics and environmental factors such as light and temperature affecting hormone levels (Davis, 2009;Domagalska et al., 2010). Studies in Arabidopsis thaliana and other angiosperm species including Antirrhinum majus, Petunia hybrid, and Oryza sativa have helped to elucidate the complex network regulated tightly during flower development (Ó'Maoiléidigh et al., 2014). Based on similarity searches, most of the flowering genes were found to be conserved in some legume species (Hecht et al., 2005). Transcription factors play a crucial role in flower development. The regulatory role of MADS-box transcription factors in regulating floral organ development is well demonstrated in soybean and chickpea (Jung et al., 2012;Singh et al., 2013). To date, several proteomic analyses have been employed to identify proteins involved in plant development. However, only a few proteomics studies on flower development are available (Dafny-Yelin et al., 2005;Ahsan and Komatsu, 2009). Analysis of rose petal proteomes showed that energy, cell rescue, and metabolism-related proteins were differentially regulated during development (Dafny-Yelin et al., 2005). The differential expression of several proteins involved in several biological processes such as energy metabolism, secondary metabolism, and signal transduction pathways provides a good example of a protein networks during flower development of soybean (Ahsan and Komatsu, 2009). Zhang et al. (2013) showed the differential expression of several proteins involved in metabolism, protein fate, signal transduction, cellular transport, and biogenesis during floral initiation in Agapanthus praecox. Recently, Zhang et al. (2016) demonstrated that the largest proportions of identified proteins were involved in metabolism, protein fate, and cell rescue during the flowering transition in Crocus sativus.Fabaceae is a large family comprising three subfamilies including Caesalpinioideae, Mimosoideae,

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“…Two-dimensional gel electrophoresis (2DGE) is increasingly used to gain new insights into plant functioning (Lery et al 2008), as it allows the separation of several hundreds of gene products in a single gel and their subsequent identification and quantification. In recent years 2-DGE has been applied to the analysis of plant development (Hochholdinger et al 2006, Mechin et al 2004), deciphering of biotic or abiotic stress (Vincent et al 2005) and characterization of plant mutants (Sorin et al 2006). This paper is the first report of a proteomic analysis of a somatic hybrid between Brassica oleracea and Brassica campestris, and mainly focusses on evidence of differential protein expression in the leaves of this hybrid and the parent species.…”

Section: Introductionmentioning

confidence: 99%

A Proteomic Analysis of Leaf Proteins Expressed in a Brassica Somatic Hybrid and its two Parental Species

Hossain

Evans

et al. 2013

Plant Tissue Cult. & Biotech.

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Cabbage (Brassica oleracea L.) is one of the major vegetable crops in the world. Several somatic hybrids between cabbage and Chinese cabbage have been produced. These show hybrid vigor. To identify differences in protein expression that might be relevant to hybrid characteristics of interest, proteins from leaves of a somatic hybrid and its two parents were subjected to 1D and 2D gel analyses, followed by MALDI-TOF and MS/MS of selected spots. 1D gel analysis of the leaf proteins at 14th day post-germination (14 DPG) showed very similar profiles for all three Brassicas. However, upon 2D gel analysis, several differentially expressed protein spots from leaves were discerned. Of the five differentially expressed leaf proteins examined, all the protein spots were photosynthesisrelated , including RbcS (the rubisco small subunit). It is possible that differential expression of these photosynthesis-related proteins may relate to the improved growth of the somatic hybrid.

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Proteomic dissection of plant development

Cited by 48 publications

References 94 publications

Proteomic Analyses of Cells Isolated by Laser Microdissection

Proteomic Analyses of Cells Isolated by Laser Microdissection

Proteomic analysis of flowers at two developmental stages in Thermopsis turcica (Fabaceae)

A Proteomic Analysis of Leaf Proteins Expressed in a Brassica Somatic Hybrid and its two Parental Species

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