Proteomic identification and physicochemical characterisation of paramyosin and collagen from octopus (Octopus vulgaris) and jumbo squid (Dosidicus gigas)

Abstract

To perform improvements in food science, it is fundamental to understand the physicochemical properties of proteins since their interaction with other macromolecules plays an essential role in food systems. Collagen and paramyosin help in the maintenance of the matrix structure cells, the textural behaviour and the technical functionality of the protein concentrates; because of this, their identification and characterisation are necessary. Cephalopods species have shown differences in the distribution of its muscle fibres. The amino acid profile of jumbo squid showed a high content of glycine and hydroxyproline, while octopus showed a high content of acidic amino acids. The thermal profile of jumbo squid showed an endothermic transition at 117°C, which octopus did not present. Moreover, the proteomic identification confirms the identity of paramyosin with 33% coverage to paramyosin from Dosidicus gigas and a 4% coverage to collagen type II from Sepia pharaonis on octopus and jumbo squid, respectively.

Distinctive characteristics of collagen and gelatin extracted from Dosidicus gigas skin

“…The characteristics of collagen or gelatin not only depended on the raw material, but also on the extraction conditions such as temperature, pH and time (Tapia-Vasquez et al, 2020). The native structure of collagen was extremely sensitive to thermal treatment, especially for the type I collagen that mainly existed in skin, which was even unstable at body temperature (37°C) (Leikina et al, 2002).…”

“…As shown in Table 1, the content of hydroxyproline in PSC (10.3%) was higher than that in other two proteins, indicating its structure stability. In fact, the content of hydroxyproline has been considered as an indicator for evaluating the quality of collagen resources (Tapia-Vasquez et al, 2020).…”

Identification of octopus peptide and its promotion of β‐casein synthesis in a mouse mammary epithelial cell line

“…Octopus ( Octopus vulgaris ) is a cephalopod mollusk distributed on temperate and tropical waters around the world. It is appreciated in the food industry due to their high protein content, high nutritional value, and various bioactivity such as antidiabetic, antihyperlipidemic, and antioxidant (Ben Slama‐Ben Salem, Bkhairia, Abdelhedi, & Nasri, 2017; Ben Slama‐Ben Salem et al., 2018alem et al., 2018; Tapia‐Vasquez et al., 2020). In China, octopus can be used as a lactation‐promoting medical effect for postpartum women with hypogalactia, according to the “Chinese Materia Medica.” It has been reported that oxytocin‐related endogenous peptides from the octopus stimulate uterine contractions and milk ejection through oxytocin receptors on the epithelial cells of mammary glands during lactation (Gimpl & Fahrenholz, 2001; Odekunle et al., 2019; Takuwa‐Kuroda, Iwakoshi‐Ukena, Kanda, & Minakata, 2003).…”

“…Octopus (Octopus vulgaris) is a cephalopod mollusk distributed on temperate and tropical waters around the world. It is appreciated in the food industry due to their high protein content, high nutritional value, and various bioactivity such as antidiabetic, antihyperlipidemic, and antioxidant (Ben Slama-Ben Salem, Bkhairia, Abdelhedi, & Nasri, 2017;Ben Slama-Ben Salem et al, 2018alem et al, 2018Tapia-Vasquez et al, 2020). In China, octopus can be used as a lactation-promoting medical effect for postpartum women with hypogalactia, according to the "Chinese Materia Medica."…”