Proteomic profiling during atherosclerosis progression using SELDI-TOF-MS: Effect of darbepoetin treatment

Dursun, Evrim; Monari, Emanuela; Cuoghi, Aurora; Bergamini, Stefania; Özben, Beste; Süleymanlar, Gültekin; Tomasi, Aldo; Özben, Tomris

doi:10.1016/j.acthis.2009.04.003

Cited by 8 publications

(5 citation statements)

References 31 publications

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“…This technology is simple, fast with highthroughput and high sensitivity [8,9]. Many protein biomarkers of certain diseases have been indicated by using SELDI-TOF MS to analyze the serum proteome [10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

The discovery and identification of a candidate proteomic biomarker of active tuberculosis

et al. 2013

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BackgroundNoninvasive and convenient biomarkers for early diagnosis of tuberculosis (TB) remain an urgent need. The aim of this study was to discover and identify potential biomarkers specific for TB.MethodsThe surface-enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF MS) combined with weak cation exchange (WCX) magnetic beads was used to screen serum samples from 180 cases of TB and 211 control subjects. A classification model was established by Biomarker Pattern Software (BPS). Candidate protein biomarkers were purified by reverse phase-high performance liquid chromatography (RP-HPLC), identified by MALDI-TOF MS, LC-MS/MS and validated using enzyme-linked immunosorbent assay (ELISA).ResultsA total of 35 discriminating m/z peaks were detected that were related to TB (P < 0.01). The model of biomarkers based on the four biomarkers (2554.6, 4824.4, 5325.7, and 8606.8 Da) was established which could distinguish TB from controls with the sensitivity of 83.3% and the specificity of 84.2%. The candidate biomarker with m/z of 2554.6 Da was found to be up-regulated in TB patients, and was identified as a fragment of fibrinogen, alpha polypeptide isoform alpha-E preproprotein. Analysis in 22 patients with TB showed increased fibrinogen degradation product (FDP) (5,005 ± 1,297 vs. 4,010 ± 1,181 ng/mL, P < 0.05) and in 142 patients showed elevated plasma fibrinogen levels.ConclusionsA diagnostic model for TB with high sensitivity and specificity was developed using mass spectrometry combined with magnetic beads. Fibrinogen was identified as a potential biomarker for TB and showed diagnostic values in clinical application.

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Section: Introductionmentioning

confidence: 99%

The discovery and identification of a candidate proteomic biomarker of active tuberculosis

et al. 2013

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“…The drug solution was freshly prepared on every injection day and administered to the mice intraperitoneally. 15 The dose was adjusted according to the body weight of each mouse during the treatment period.…”

Section: Drug Treatmentmentioning

confidence: 99%

Effects of Long- and Short-term Darbepoetin-α Treatment on Oxidative Stress, Inflammation and Endothelial Injury in Apo E Knockout Mice

et al. 2017

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“…However, a few proteomic analyses have been performed in whole serum. For example, proteomic profiling has been done on whole serum of apoE‐deficient mice during atherosclerosis progression (Dursun et al, ), as well as upon pharmacological treatment with compounds such as diarbepoetin, a synthetic erythropoietin analogue that stimulates erythropoiesis (Dursun et al, ), and nebivolol (a third‐generation vasodilatory beta‐blocker; Ozben et al, ). Moreover, the impact of the APOE3‐Leiden isoform on the protein profile has been investigated by comparing whole plasma of transgenic apoE*3‐Leiden mice versus isogenic wild‐type controls (Davidov et al, ).…”

Section: Conclusion: Technologies On the Horizonmentioning

confidence: 99%

Overview of the Measurement of Lipids and Lipoproteins in Mice

Tailleux

Staels

2011

CP Mouse Biology

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Experimental mouse models are widely used for preclinical research on dyslipidemia, atherosclerosis, and cardiometabolic diseases. This unit reports the most commonly used biochemical analysis methods available to determine the lipid and lipoprotein phenotype in the mouse. The discussed methods include: the qualitative and quantitative analysis of lipids, apolipoproteins, and lipoproteins (with a specific emphasis on species-specificities of mice and humans), and the activity assay of major enzymes involved in lipoprotein remodeling (LCAT, PLTP, and LPL). The unit also discusses the most frequently used functional tests to analyze lipid/lipoprotein metabolism in vivo, including triglyceride metabolism, reverse cholesterol transport, intestinal lipid absorption, and secretion. Curr. Protoc. Mouse Biol. 1:265-277 © 2011 by John Wiley & Sons, Inc.

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Proteomic profiling during atherosclerosis progression using SELDI-TOF-MS: Effect of darbepoetin treatment

Cited by 8 publications

References 31 publications

The discovery and identification of a candidate proteomic biomarker of active tuberculosis

The discovery and identification of a candidate proteomic biomarker of active tuberculosis

Effects of Long- and Short-term Darbepoetin-α Treatment on Oxidative Stress, Inflammation and Endothelial Injury in Apo E Knockout Mice

Overview of the Measurement of Lipids and Lipoproteins in Mice

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