Proteomic Profiling for Metabolic Pathways Involved in Interactive Effects of Elevated Carbon Dioxide and Nitrogen on Leaf Growth in a Perennial Grass Species

Yu, Jingjin; Fan, Ningli; Li, Ran; Zhuang, Lili; Xu, Qian; Huang, Bingru

doi:10.1021/acs.jproteome.8b00951

Cited by 7 publications

(6 citation statements)

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“…Total protein of 8 h imbibed seeds was extracted using phenol-based method and the concentrations were measured with bovine serum albumin. Next, 100 μg protein was used for iTRAQ labelling proteomics analysis [ 55 ]. Protein digestion and labelling were performed according to the manufacturer's instructions (AB SCIEX, Concord, ON).…”

Section: Methodsmentioning

confidence: 99%

“…Data acquisition was performed with a TripleTOF 5600 mass spectrometer (MS) (AB SCIEX, Concord, ON) fitted with a Nanospray III source (AB SCIEX, Concord, ON). Data were acquired on the parameters as previously reported [ 55 ]. The original mass data were processed with ProteinPilot 5.0 (AB SCIEX, Concord, ON) and searched against the Uniprot protein database ( http://www.uniprot.org ) and G. hirsutum TM-1 protein FASTA ( https://www.cottongen.org/ ) [ 56 ].…”

Section: Methodsmentioning

confidence: 99%

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Suppression of GhGLU19 encoding β-1,3-glucanase promotes seed germination in cotton

et al. 2022

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Background In eudicots, germination begins with water uptake by the quiescent dry seed and is greatly related to the permeability of micropyle enriched callose layers. Once imbibition starts, seeds undergo a cascade of physiological, biochemical, and molecular events to initiate cellular activities. However, the effects of callose on water uptake and following seed metabolic events during germination are largely unknown. Cotton (Gossypium hirsutum) is a eudicot plant with natural fiber and edible oil production for humans. Here, we addressed this question by examining the role of GhGLU19, a gene encoding β-1,3-glucanase, in cotton seed germination. Results GhGLU19 belongs to subfamily B and was expressed predominately in imbibed seeds and early seedlings. Compared to wild type, GhGLU19-suppressing and GhGLU19-overexpressing transgenic cotton lines showed the higher and lower seed germination percentage, respectively. Callose was enriched more at inner integument (ii) than that in embryo and seed coat in cotton seeds. In GhGLU19-suppressing lines, callose at ii of cotton seeds was greatly increased and brought about a prolonged water uptake process during imbibition. Both proteomic and transcriptomic analysis revealed that contrary to GhGLU19-overexpressing lines, the glycolysis and pyruvate metabolism was decreased, and abscisic acid (ABA) biosynthesis related genes were downregulated in imbibed seeds of GhGLU19-suppressing lines. Also, endogenous ABA was significantly decreased in GhGLU19-suppressing line while increased in GhGLU19-overexpressing line. Conclusions Our results demonstrate that suppression of GhGLU19 improves cotton seed germination via accumulating callose of inner integument, modulating glycolysis and pyruvate metabolism, and decreasing ABA biosynthesis. This study provides a potential way for improving germination percentage in cotton seed production, and other eudicot crops.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Suppression of GhGLU19 encoding β-1,3-glucanase promotes seed germination in cotton

et al. 2022

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“…A rolling collision energy voltage was used for CID fragmentation for MS/MS spectra acquisitions. Mass was dynamically excluded for 22 s (Yu et al, 2019).…”

Section: Lc-ms/ms Analysismentioning

confidence: 99%

Hepatic Proteomic Changes and Sirt1/AMPK Signaling Activation by Oxymatrine Treatment in Rats With Non-alcoholic Steatosis

Hong

Chen

et al. 2020

Front. Pharmacol.

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Background: Currently, active ingredients of herbal extracts that can suppress lipid accumulation in the liver have been considered a potential treatment option for nonalcoholic fatty liver disease. Methods: Steatosis rat model was created by high fat and high sucrose diet feeding and treated with oxymatrine (OMT). Serum biochemical parameters, liver histology and lipid profiles were examined. Hepatic differentially expressed proteins (DEPs) which were significantly changed by OMT treatment were identified by iTRAQ analysis. The expressions of representative DEPs, Sirt1 and AMPKα were evaluated by western blotting. Results: OMT significantly reduced the body weight and liver weight of steatosis animals, decreased the serum levels of triglyceride and total cholesterol as well as the hepatic triglyceride and free fatty acid levels, and effectively alleviated fatty degeneration in the liver. A list of OMT-related DEPs have been screened and evaluated by bioinformatics analysis. OMT significantly decreased the expressions of L-FABP, Plin2, FASN and SCD1 and increased Sirt1 expression and AMPKα phosphorylation in the liver of rats with steatosis. Conclusion: The present study has confirmed the significant efficacy of OMT for improving steatosis and revealed hepatic proteomic changes and Sirt1/AMPK signaling activation by OMT treatment in rats with steatosis.

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“…A large number of studies reported that elevated CO 2 could promote plant growth and development, including perennial grass species, such as tall fescue ( Festuca arundinacea Schreb.) [ 13 , 14 , 15 , 16 ], Kentucky bluegrass ( Poa pratensis L.) [ 17 , 18 ], and bermudagrass ( Cynodon dactylo n (L.) Pers.) [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Reallocation of Soluble Sugars and IAA Regulation in Association with Enhanced Stolon Growth by Elevated CO2 in Creeping Bentgrass

et al. 2022

Plants

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Extensive stolon development and growth are superior traits for rapid establishment as well as post-stress regeneration in stoloniferous grass species. Despite the importance of those stoloniferous traits, the regulation mechanisms of stolon growth and development are largely unknown. The objectives of this research were to elucidate the effects of the reallocation of soluble sugars for energy reserves and endogenous hormone levels for cell differentiation and regeneration in regulating stolon growth of a perennial turfgrass species, creeping bentgrass (Agrostis stolonifera L.). Plants were grown in growth chambers with two CO2 concentrations: ambient CO2 concentration (400 ± 10 µmol mol−1) and elevated CO2 concentration (800 ± 10 µmol mol−1). Elevated CO2 enhanced stolon growth through increasing stolon internode number and internode length in creeping bentgrass, as manifested by the longer total stolon length and greater shoot biomass. The content of glucose, sucrose, and fructose as well as endogenous IAA were accumulated in stolon nodes and internodes but not in leaves or roots under elevated CO2 concentration. These results illustrated that the production and reallocation of soluble sugars to stolons as well as the increased level of IAA in stolon nodes and internodes could contribute to the enhancement of stolon growth under elevated CO2 in creeping bentgrass.

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Proteomic Profiling for Metabolic Pathways Involved in Interactive Effects of Elevated Carbon Dioxide and Nitrogen on Leaf Growth in a Perennial Grass Species

Cited by 7 publications

References 50 publications

Suppression of GhGLU19 encoding β-1,3-glucanase promotes seed germination in cotton

Suppression of GhGLU19 encoding β-1,3-glucanase promotes seed germination in cotton

Hepatic Proteomic Changes and Sirt1/AMPK Signaling Activation by Oxymatrine Treatment in Rats With Non-alcoholic Steatosis

Reallocation of Soluble Sugars and IAA Regulation in Association with Enhanced Stolon Growth by Elevated CO2 in Creeping Bentgrass

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