Proteomic profiling of a parasitic wasp exposed to constant and fluctuating cold exposure

Colinet, Hervé; Nguyen, Thi Thuy An; Cloutier, Conrad; Michaud, Dominique; Hance, Thierry

doi:10.1016/j.ibmb.2007.07.004

Cited by 109 publications

(101 citation statements)

References 68 publications

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“…As the rearing temperature experienced by short-day females (20°C) was lower than that experienced by long-day females (25°C), this result is consistent with the proteomic report that a Gasp precursor is up-regulated under constant low temperature conditions in a parasitic wasp (Colinet et al, 2007). Gasp, a peritrophin that interacts with chitin, is known to be expressed in the ovary and embryonic tracheae (Barry et al, 1999).The biological functions of Gasp are still poorly understood although peritrophins may be functionally diverse.…”

Section: Discussionsupporting

confidence: 90%

“…Given that some Drosophila microtubule-associated protein is required for the structure and function of cytoplasmic microtubules during oogenesis (Moon & Hazelrigg, 2004) fk506-binding protein might be related to oogenesis in the ovaries of non-diapause insects. Differential expressions of cytoskeleton components and cytoskeleton rearrangement, particularly the re-distribution of actin are reported to play a role in insect survival at low temperatures (Kim et al, 2006;Kim & Denlinger, 2009;Colinet et al, 2007). Together, increases in the abundance of the 3 actinbinding proteins twinstar, muscle protein 20-like protein and GA13413-PA and decrease in the abundance of fk506-binding protein may underscore cytoskeleton alterations in the ovaries of short-day females.…”

Section: Discussionmentioning

confidence: 99%

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Proteomic approach to understanding the maternal effect in the flesh fly, Sarcophaga bullata (Diptera: Sarcophagidae)

Li¹,

Wang²,

Liang³

et al. 2012

Eur. J. Entomol.

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Abstract. Progeny of the flesh fly Sarcophaga bullata exposed to short day length show a maternal effect that prevents the expression of pupal diapause. Although ecological aspects of this effect are well studied, not enough is known about the molecular mechanisms underlying this maternal effect. In this study, two-dimensional electrophoresis was performed to detect differences of the abundance of certain proteins in the ovaries of this fly kept under long day and short day conditions for 2 days after eclosion. Eleven proteins that were abundant and showed significant changes were selected for mass spectrometric identification. Ovary proteins that increased in abundance under short-day conditions were similar to twinstar CG4254-PA, muscle protein 20-like protein, GA13413-PA, gene analogous to small peritrophins (Gasp CG10287-PA) and Ribosomal protein LP1 CG4087-PA. Ovary proteins that decreased in abundance under short-day conditions were similar to the ATP synthase beta subunit, fk506-binding protein and storage protein-binding protein. The 2-D proteome maps included 2 additional unknown proteins that were more abundant and 1 that was less abundant in the ovaries of 2-day old short-day females. Twinstar CG4254-PA, muscle protein 20-like protein and GA13413-PA harbour an actin-binding domain. That the 3 actin-binding proteins increase in abundance suggests that it is likely that an alteration in the actin cytoskeleton is involved in this maternal effect in the flesh fly.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Proteomic approach to understanding the maternal effect in the flesh fly, Sarcophaga bullata (Diptera: Sarcophagidae)

Li¹,

Wang²,

Liang³

et al. 2012

Eur. J. Entomol.

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“…Since then, technologies have been developing fast and state of the art methods now enable identification and quantification of sometimes thousands of proteins in organisms such as Escherichia coli (Soufi et al, 2015). In spite of this, studies on thermal acclimation responses in arthropods have rarely identified more than a few hundred proteins (Colinet et al, 2007(Colinet et al, , 2013Pedersen et al, 2010;Wei et al, 2015), making investigation of complex physiological changes in response to thermal acclimation difficult.…”

Section: Introductionmentioning

confidence: 99%

Proteomic data reveals a physiological basis for costs and benefits associated with thermal acclimation

Kristensen

Kjeldal

Schou

et al. 2016

Journal of Experimental Biology

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Physiological adaptation through acclimation is one way to cope with temperature changes. Biochemical studies on acclimation responses in ectotherms have so far mainly investigated consequences of short-term acclimation at the adult stage and focussed on adaptive responses. Here, we assessed the consequences of rearing Drosophila melanogaster at low (12°C), benign (25°C) and high (31°C) temperatures. We assessed cold and heat tolerance and obtained detailed proteomic profiles of flies from the three temperatures. The proteomic profiles provided a holistic understanding of the underlying biology associated with both adaptive and non-adaptive temperature responses. Results show strong benefits and costs across tolerances: rearing at low temperature increased adult cold tolerance and decreased adult heat tolerance and vice versa with development at high temperatures. In the proteomic analysis, we were able to identify and quantify a large number of proteins compared with previous studies on ectotherms (1440 proteins across all replicates and rearing regimes), enabling us to extend the proteomic approach using enrichment analyses. This gave us detailed information on individual proteins, as well as pathways affected by rearing temperature, pinpointing potential mechanisms responsible for the strong costs and benefits of rearing temperature on functional phenotypes. Several well-known heat shock proteins, as well as proteins not previously associated with thermal stress, were among the differentially expressed proteins. Upregulation of proteasome proteins was found to be an important adaptive process at high-stress rearing temperatures, and occurs at the expense of downregulation of basal metabolic functions.

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“…In addition to being ecologically relevant, studies of RCE can also reveal novel adaptations for cold survival. For example, in the parasitic wasp Aphidius colimani, the proteome of wasps exposed to RCE was distinct from those exposed to a single bout of low temperature (Colinet et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Survival and energetic costs of repeated cold exposure in the Antarctic midge, Belgica antarctica: a comparison between frozen and supercooled larvae

Teets

Kawarasaki

Lee

et al. 2011

Journal of Experimental Biology

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There was an error published in J. Exp. Biol. 214,[806][807][808][809][810][811][812][813][814] In calculating the metabolite levels (i.e. lipid, glycogen, trehalose, glucose and glycerol) and energy content values (Figs2 and 3), an incorrect correction factor was applied in the last step of the calculation. The correct metabolite and energy content values can be obtained by multiplying the values that appear in the manuscript by a factor of 2.

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Proteomic profiling of a parasitic wasp exposed to constant and fluctuating cold exposure

Cited by 109 publications

References 68 publications

Proteomic approach to understanding the maternal effect in the flesh fly, Sarcophaga bullata (Diptera: Sarcophagidae)

Proteomic approach to understanding the maternal effect in the flesh fly, Sarcophaga bullata (Diptera: Sarcophagidae)

Proteomic data reveals a physiological basis for costs and benefits associated with thermal acclimation

Survival and energetic costs of repeated cold exposure in the Antarctic midge, Belgica antarctica: a comparison between frozen and supercooled larvae

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