Proteomic profiling of human embryonic stem cell-derived microvesicles reveals a risk of transfer of proteins of bovine and mouse origin

Kubíková, Iva; Konečná, Hana; Šedo, Ondřej; Zdráhal, Zbyněk; Řehulka, Pavel; Hříbková, Hana; Řehulková, Helena; Hampl, Aleš; Chmelı́k, Josef; Dvořák, Petr

doi:10.1080/14653240802595531

Cited by 23 publications

(9 citation statements)

References 53 publications

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“…Jeong et al [ 60 ] showed that MVs engineered from ESCs could enhance cell proliferation and potentially contribute to recovery or wound healing process of tissues. Simultaneously, we should be prudent toward the clinical use of MVs derived from the ESCs, which was best exemplified by Kubikova et al's study [ 61 ] in which they had a proteomic profiling of MVs derived from human ESCs. This study confirmed the role of MVs in communicating between human ESCs.…”

Section: Escsmentioning

confidence: 99%

Exosomes and Their Therapeutic Potentials of Stem Cells

Han

Sun

Liu

et al. 2015

Stem Cells International

193

143

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Exosomes, a group of vesicles originating from the multivesicular bodies (MVBs), are released into the extracellular space when MVBs fuse with the plasma membrane. Numerous studies indicate that exosomes play important roles in cell-to-cell communication, and exosomes from specific cell types and conditions display multiple functions such as exerting positive effects on regeneration in many tissues. It is widely accepted that the therapeutic potential of stem cells may be mediated largely by the paracrine factors, so harnessing the paracrine effects of stem and progenitor cells without affecting these living, replicating, and potentially pluripotent cell populations is an advantage in terms of safety and complexity. Ascending evidence indicated that exosomes might be the main components of paracrine factors; thus, understanding the role of exosomes in each subtype of stem cells is far-reaching. In this review, we discuss the functions of exosomes from different types of stem cells and emphasize the therapeutic potentials of exosomes, providing an alternative way of developing strategies to cure diseases.

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Section: Escsmentioning

confidence: 99%

Exosomes and Their Therapeutic Potentials of Stem Cells

Han

Sun

Liu

et al. 2015

Stem Cells International

193

143

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“…The feeder cells serve fibroblasts of different origin. At present, many researchers use human fibroblasts to prevent infection of human ESCs by retroviruses or other rodent pathogens (Martin et al, 2005;Skottman et al, 2006;Kubikova et al, 2009). As feeders, there has been wide use of human foreskin fibroblasts and mesenchymal stem cells (MSCs) derived from various sources, particularly from human ESCs (Olivier et al, 2006;Lian et al, 2007;Choo et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Comparative characteristics of new lines of mesenchymal stem cells derived from human embryonic stem cells, bone marrow, and foreskin

et al. 2012

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New human nonimmortalized fibroblast like cell lines were derived from various sources: from embryonic stem cells (ESCs) (the SC5 MSC and SC3a MSC lines), from bone marrow of a 5 to 6 week old fetus (the FetMSC line), and from foreskin of a 3 year old child (the FRSN line). All the lines are suc cessfully used as feeders during cultivation of human ESCs. The mean doubling time of the cell populations fluctuates depending on the line from 25.5 h in the SC5 MSC line to 38.8 h in the SC3a MSC line. The growth curves indicate active cell proliferation of all lines. Numerical and structural karyotypical analysis has shown these lines to have a normal karyotype: 46, XX (SC5 MSC and SC3a MSC) and 46, XY (FetMSC and FRSN). To determine the status of these lines, comparative analysis of surface markers was performed with the aid of flow cytofluorimetry, and expression of the c antigens characteristic of human mesenchymal stem cells (MSCs) was revealed: CD44, CD73, CD90, CD105, and HLA ABC and the absence of expression of CD34 and HLA DR. Interlinear differences in the expression level of the marker CD117 (c kit) were revealed. Immunofluorescent and cytofluorimetric analysis of expression of surface markers and transcrip tion factor Oct 4 that are characteristic of human ESCs has shown that, in all four lines, expression of TRA 1 60 and Oct 4 is absent, whereas in expression of SSEA 4 there are observed the interlinear differ ences not depending on the origin of cells. At present it is not yet clear whether the revealed interlinear dif ferences affect essentially the functional status of mesenchymal stem cells. Immunofluorescent analysis in cells of all lines showed expression of markers of early differentiation into derivatives of three germ layers characterizing ESCs, which might possibly provide wide MSC possibilities during reparation of different tis sue damages, depending on the corresponding microenvironment. The capability of cells of all lines for directed differentiation into the adipogenic and osteogenic directions was revealed.

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“…The cells showed a high level of pluripotent markers despite being cultured in a pointedly differentiation inducing medium. The cells showed low CFU-c or clonogenic potential and the cells show resistance to differentiation into the desired cell phenotype although other ESC lines such as H1, H7, KCL002-WT4, Shef-6 and others do [36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52][53][54][55].…”

Section: Discussionmentioning

confidence: 99%

Heterogeneity in Human Embryonic Stem Cells May Prevent Endodermal Guided Differentiation

Surajlata¹

2015

J Stem Cell Res Ther

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Proteomic profiling of human embryonic stem cell-derived microvesicles reveals a risk of transfer of proteins of bovine and mouse origin

Cited by 23 publications

References 53 publications

Exosomes and Their Therapeutic Potentials of Stem Cells

Exosomes and Their Therapeutic Potentials of Stem Cells

Comparative characteristics of new lines of mesenchymal stem cells derived from human embryonic stem cells, bone marrow, and foreskin

Heterogeneity in Human Embryonic Stem Cells May Prevent Endodermal Guided Differentiation

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