Proteomic Profiling of Inflammatory Signaling Molecules in the Tears of Patients on Chronic Glaucoma Medication

Wong, Tina; Zhou, Lanlan; Li, Jing; Tong, Louis; Zhao, Shengyuan; Li, Xiao Rong; Yu, Shang Juan; Koh, Siew Kwan; Beuerman, Roger W.

doi:10.1167/iovs.10-6532

Cited by 92 publications

(72 citation statements)

References 40 publications

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“…Analysis of tears from blepharitis patients has shown that several tear proteins are down-regulated including albumin and lacritin precursors and lysozyme (Koo et al, 2005). Proteomics of the tears of patients on chronic glaucoma medication showed downregulation of different set of proteins including mammaglobin B, S100 proteins and proline-rich 4 (Wong et al, 2011). Other papers in this issue provide more details on the tear proteome and other functional proteins.…”

Section: Tear Filmmentioning

confidence: 98%

“…ml) samples. Through the development of omics techniques for biology, tear film proteomics (de Souza et al, 2006;Zhou et al, 2012), lipidomics (Rantamaki et al, 2011), glycomics (Vieira et al, 2012) and metabolomics (Chen et al, 2011) are now being used to characterize the normal composition of the tear film and how this may be modulated in diseases (Koo et al, 2005;Lam et al, 2011;Wong et al, 2011). The secretions of the lacrimal gland are the major source of tear proteins and fluid and those of the meibomian glands are the major source of tear lipids.…”

Section: Tear Filmmentioning

confidence: 99%

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Diet, nutraceuticals and the tear film

Jalbert

2013

Experimental Eye Research

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Section: Tear Filmmentioning

confidence: 98%

Section: Tear Filmmentioning

confidence: 99%

Diet, nutraceuticals and the tear film

Jalbert

2013

Experimental Eye Research

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“…Chemical labeling with iTRAQ or TMT makes possible the labeling of up to 8 or 10 different samples with an isobaric label [38][39][40][41][42][43]. The label is constructed in that way that the m/z values of the peptides originating from different samples (precursor ions) are the same but during fragmentation the labeling group is released and the rest of the peptide can be used for MS/ MS based protein identification.…”

Section: Proteomic Methods Applied For Dr Diagnosismentioning

confidence: 99%

Diabetic retinopathy: Proteomic approaches to help the differential diagnosis and to understand the underlying molecular mechanisms

Csősz

Deák

Kalló

et al. 2017

Journal of Proteomics

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a b s t r a c t a r t i c l e i n f oDiabetic retinopathy is the most common diabetic eye disease and a leading cause of blindness among patients with diabetes. The appearance and the severity of the symptoms correlate with the duration of diabetes and poor blood glucose level management. Diabetic retinopathy is also categorized as a chronic low-level inflammatory disease; the high blood glucose level promotes the accumulation of the advanced glycation end products and leads to the stimulation of monocytes and macrophages. Examination of protein level alterations in tears using state-of the art proteomics techniques have identified several proteins as possible biomarkers for the different stages of the diabetic retinopathy. Some of the differentially expressed tear proteins have a role in the barrier function of tears linking the diabetic retinopathy with another eye complication of diabetes, namely the diabetic keratopathy resulting in impaired wound healing. Understanding the molecular events leading to the eye complications caused by hyperglycemia may help the identification of novel biomarkers as well as therapeutic targets in order to improve quality of life of diabetic patients. Biological significance: Diabetic retinopathy (DR), the leading cause of blindness among diabetic patients can develop without any serious symptoms therefore the early detection is crucial. Because of the increasing prevalence there is a high need for improved screening methods able to diagnose DR as soon as possible. The non-invasive collection and the relatively high protein concentration make the tear fluid a good source for biomarker discovery helping the early diagnosis. In this work we have reviewed the administration of advanced proteomics techniques used in tear biomarker studies and the identified biomarkers with potential to improve the already existing screening methods for DR detection.

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“…The proteome of human tears has been well-profiled and thousands of proteins have been identified (144,145). Proteins have been demonstrated to be differentially expressed in the tears of patients with dry eye syndrome (146), keratoconus (147), diabetic retinopathy (148) and in those treated with chronic glaucoma medication (149). Studies investigating the PTM alterations in tears have been performed using proteomic technology.…”

Section: Ptms In Eye Researchmentioning

confidence: 99%

Post-translational modifications and their applications in eye research

Chen

Lam

Liu

et al. 2017

Molecular Medicine Reports

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Abstract. Gene expression is the process by which genetic information is used for the synthesis of a functional gene product, and ultimately regulates cell function. The increase of biological complexity from genome to proteome is vast, and the post-translational modification (PTM) of proteins contribute to this complexity. The study of protein expression and PTMs has attracted attention in the post-genomic era. Due to the limited capability of conventional biochemical techniques in the past, large-scale PTM studies were technically challenging. The introduction of effective protein separation methods, specific PTM purification strategies and advanced mass spectrometers has enabled the global profiling of PTMs and the identification of a targeted PTM within the proteome. The present review provides an overview of current proteomic technologies being applied in eye research, with a particular focus on studies of PTMs in ocular tissues and ocular diseases.

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Proteomic Profiling of Inflammatory Signaling Molecules in the Tears of Patients on Chronic Glaucoma Medication

Cited by 92 publications

References 40 publications

Diet, nutraceuticals and the tear film

Diet, nutraceuticals and the tear film

Diabetic retinopathy: Proteomic approaches to help the differential diagnosis and to understand the underlying molecular mechanisms

Post-translational modifications and their applications in eye research

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