Proteomic Profiling of the Influence of Iron Availability on <i>Cryptococcus gattii</i>

Crestani, Juliana; Carvalho, Paulo C.; Han, Xuemei; Seixas, Adriana; Broetto, Leonardo; Fischer, Juliana de Saldanha da Gama; Staats, Charley Christian; Schrank, Augusto; Yates, John R.; Vainstein, Marilene Henning

doi:10.1021/pr2005296

Cited by 17 publications

(17 citation statements)

References 107 publications

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“…The identification of the proteins aconitase (an enzyme involved in the metabolism of carbohydrates) and Wos2 (a cochaperone of Hsp90) corroborates the results of a study by Crestani et al [73], which showed that aconitase and Wos2 expression levels increase in C. gattii (strain R265) in response to a lack of iron in the culture medium. It is important to note that the common factors between our study and that of Crestani et al [73] are the stress conditions to which C. gattii was exposed and that Wos2 has been suggested to be an early marker of C. gattii infection in koalas [56].…”

Section: Discussionsupporting

confidence: 87%

“…It is important to note that the common factors between our study and that of Crestani et al [73] are the stress conditions to which C. gattii was exposed and that Wos2 has been suggested to be an early marker of C. gattii infection in koalas [56]. Similar to enolase, the protein cofilin identified herein may play a relevant role in the process of the fungus spreading to the CNS.…”

Section: Discussionsupporting

confidence: 62%

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Immunoproteomics and Immunoinformatics Analysis of Cryptococcus Gattii : Novel Candidate Antigens for Diagnosis

et al. 2013

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Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 62%

Immunoproteomics and Immunoinformatics Analysis of Cryptococcus Gattii : Novel Candidate Antigens for Diagnosis

et al. 2013

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“…30 Proteins were solubilized by vortexing five times for 1 min each at intervals of 1 min on ice and centrifuged (13 000 rpm for 20 min). Supernatants were collected, and the remaining cell debris were suspended in the same buffer, followed by the same protocol.…”

Section: Materials and Methodsmentioning

confidence: 99%

Proteomic Profile of Cryptococcus neoformans Biofilm Reveals Changes in Metabolic Processes

et al. 2014

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Cryptococcus neoformans, a pathogenic yeast, causes meningoencephalitis, especially in immunocompromised patients, leading in some cases to death. Microbes in biofilms can cause persistent infections, which are harder to treat. Cryptococcal biofilms are becoming common due to the growing use of brain valves and other medical devices. Using shotgun proteomics we determine the differences in protein abundance between biofilm and planktonic cells. Applying bioinformatic tools, we also evaluated the metabolic pathways involved in biofilm maintenance and protein interactions. Our proteomic data suggest general changes in metabolism, protein turnover, and global stress responses. Biofilm cells show an increase in proteins related to oxidation–reduction, proteolysis, and response to stress and a reduction in proteins related to metabolic process, transport, and translation. An increase in pyruvate-utilizing enzymes was detected, suggesting a shift from the TCA cycle to fermentation-derived energy acquisition. Additionally, we assign putative roles to 33 proteins previously categorized as hypothetical. Many changes in metabolic enzymes were identified in studies of bacterial biofilm, potentially revealing a conserved strategy in biofilm lifestyle.

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“…[40]. Samples were then suspended in the buffer containing 50 mM Tris–HCl, pH 7.5, 1 mM EDTA, 1 mM PMSF.…”

Section: Methodsmentioning

confidence: 99%

Proteogenomic analysis of pathogenic yeast Cryptococcus neoformans using high resolution mass spectrometry

et al. 2014

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BackgroundCryptococcus neoformans, a basidiomycetous fungus of universal occurrence, is a significant opportunistic human pathogen causing meningitis. Owing to an increase in the number of immunosuppressed individuals along with emergence of drug-resistant strains, C. neoformans is gaining importance as a pathogen. Although, whole genome sequencing of three varieties of C. neoformans has been completed recently, no global proteomic studies have yet been reported.ResultsWe performed a comprehensive proteomic analysis of C. neoformans var. grubii (Serotype A), which is the most virulent variety, in order to provide protein-level evidence for computationally predicted gene models and to refine the existing annotations. We confirmed the protein-coding potential of 3,674 genes from a total of 6,980 predicted protein-coding genes. We also identified 4 novel genes and corrected 104 predicted gene models. In addition, our studies led to the correction of translational start site, splice junctions and reading frame used for translation in a number of proteins. Finally, we validated a subset of our novel findings by RT-PCR and sequencing.ConclusionsProteogenomic investigation described here facilitated the validation and refinement of computationally derived gene models in the intron-rich genome of C. neoformans, an important fungal pathogen in humans.

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Proteomic Profiling of the Influence of Iron Availability on Cryptococcus gattii

Cited by 17 publications

References 107 publications

Immunoproteomics and Immunoinformatics Analysis of Cryptococcus Gattii : Novel Candidate Antigens for Diagnosis

Immunoproteomics and Immunoinformatics Analysis of Cryptococcus Gattii : Novel Candidate Antigens for Diagnosis

Proteomic Profile of Cryptococcus neoformans Biofilm Reveals Changes in Metabolic Processes

Proteogenomic analysis of pathogenic yeast Cryptococcus neoformans using high resolution mass spectrometry

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