Proteomic profiling reveals key cancer progression modulators in shed microvesicles released from isogenic human primary and metastatic colorectal cancer cell lines

Suwakulsiri, Wittaya; Rai, Alin; Xu, Rong; Chen, Maoshan; Greening, David W.; Simpson, Richard J.

doi:10.1016/j.bbapap.2018.11.008

Cited by 28 publications

(50 citation statements)

References 104 publications

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“…To deal with the limited success of 'targeted therapies', quantitative proteomics, together with other major technological and conceptual developments, has reinforced the search for characteristic features of the adhesive-migratory phenotype of malignant cells (9). Some other examples of important contributions include the study of RNA-protein complexes (10), stem cell plasticity (11), chromatin remodeling (12) and more recently, the regulation of mitochondrial function and dynamics (13), shed microvesicles biology (14) or mechanisms of radioresistance (15).…”

Section: Introductionmentioning

confidence: 99%

Biomarkers of tumor invasiveness in proteomics (Review)

et al. 2020

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Over the past two decades, quantitative proteomics has emerged as an important tool for deciphering the complex molecular events involved in cancers. The number of references involving studies on the cancer metastatic process has doubled since 2010, while the last 5 years have seen the development of novel technologies combining deep proteome coverage capabilities with quantitative consistency and accuracy. To highlight key findings within this huge amount of information, the present review identified a list of tumor invasive biomarkers based on both the literature and data collected on a biocollection of experimental cell lines, tumor models of increasing invasiveness and tumor samples from patients with colorectal or breast cancer. Crossing these different data sources led to 76 proteins of interest out of 1,245 mentioned in the literature. Information on these proteins can potentially be translated into clinical prospects, since they represent potential targets for the development and evaluation of innovative therapies, alone or in combination. Herein, a systematical review of the biology of each of these proteins, including their specific subcellular/extracellular or multiple localizations is presented. Finally, as an important advantage of quantitative proteomics is the ability to provide data on all these molecules simultaneously in cell pellets, body fluids or paraffin-embedded sections of tumors/invaded tissues, the significance of some of their interconnections is discussed.

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Section: Introductionmentioning

confidence: 99%

Biomarkers of tumor invasiveness in proteomics (Review)

et al. 2020

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“…We apologize for not being able to describe other methods for EV isolation or commercially available kits due to space constraints. With a need for transparency and consistency in the field of EV research, investigators are encouraged to report the chosen methods used for isolation and characterization, such as expression of sEV and LEV markers, nanoparticle tracking analysis [36,37] , tunable resistive pulse sensing, flow cytometry, laser tweezers Raman spectroscopy, digital ELISA, digital PCR and transmission electron microscopy [17,38,39] .…”

Section: Resultsmentioning

confidence: 99%

Methods for extracellular vesicle isolation from cancer cells

Salem¹,

Naranjo²,

Singh³

et al. 2020

CDR

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Cells are known to release different types of vesicles such as small extracellular vesicles (sEVs) and large extracellular vesicles (LEVs). sEVs and LEVs play important roles in intercellular communication, pre-metastatic niche formation, and disease progression; both can be detected cell culture media and biological fluids. sEVs and LEVs contain a variety of protein and RNA cargo, and they are believed to impact many biological functions of the recipient cells upon their internalization or binding to cell surface proteins. It has recently been established that standard isolation techniques, such as differential ultracentrifugation, yield a mixed population of EVs. However, density gradient ultracentrifugation has been reported to allow the isolation of sEVs without cellular debris. Here, we describe the most common methods used to isolate sEVs from cell culture medium, mouse and human plasma, and a new technique for isolating sEVs from tissues as well. This article also provides detailed procedures to isolate LEVs.

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“…In-Gel Digestion [33][34][35][36][37] FASP [38,39] Combined method In-Gel Digestion [28,35,36,[40][41][42][43][44][45][46][47][48][49][50][51] In-Sol Digestion [52][53][54] FASP [51,[55][56][57][58][59] Plasma/ Serum Ultracentrifuge In-Sol Digestion [60] FASP [56] Combined method In-Gel Digestion [43] In-Sol Digestion [44,52] FASP [51,57,58] secreted by MCF7, 30 proteins have been known to participate in breast cancer growth, metastasis, and chemoresistance. The most abundant MDA-MD-231 derived exosomal protein is β-actin, which is frequently used as a housekeeping gene in the exosome.…”

Section: Cell Ultracentrifugementioning

confidence: 99%

Exploring the key communicator role of exosomes in cancer microenvironment through proteomics

Kim

Cho

2019

Proteome Sci

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There have been many attempts to fully understand the mechanism of cancer behavior. Yet, how cancers develop and metastasize still remain elusive. Emerging concepts of cancer biology in recent years have focused on the communication of cancer with its microenvironment, since cancer cannot grow and live alone. Cancer needs to communicate with other cells for survival, and thus they secrete various messengers, including exosomes that contain many proteins, miRNAs, mRNAs, etc., for construction of the tumor microenvironment. Moreover, these intercellular communications between cancer and its microenvironment, including stromal cells or distant cells, can promote tumor growth, metastasis, and escape from immune surveillance. In this review, we summarized the role of proteins in the exosome as communicators between cancer and its microenvironment. Consequently, we present cancer specific exosome proteins and their unique roles in the interaction between cancer and its microenvironment. Clinically, these exosomes might provide useful biomarkers for cancer diagnosis and therapeutic tools for cancer treatment.

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Proteomic profiling reveals key cancer progression modulators in shed microvesicles released from isogenic human primary and metastatic colorectal cancer cell lines

Cited by 28 publications

References 104 publications

Biomarkers of tumor invasiveness in proteomics (Review)

Biomarkers of tumor invasiveness in proteomics (Review)

Methods for extracellular vesicle isolation from cancer cells

Exploring the key communicator role of exosomes in cancer microenvironment through proteomics

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