Proteomic responses of rice young panicles to salinity

Abstract

Rice (Oryza sativa) is most sensitive to salinity during the reproductive stage. We employed a proteomic approach to further understand the mechanism of plant responses to salinity at an early reproductive stage. Plants were grown in culture solution and salt stress imposed at panicle initiation. After 12 days of stress, young panicles were collected from control and salt stressed plants. The Na + and K + content of panicle and several yield components changed significantly in response to short-term salt stress. The collected panicles were sorted into three different sizes (7 6 1, 11 6 1, and 15 6 1 mm) and their proteome patterns were analyzed using 2-DE in triplicates. The expression pattern of 13 proteins significantly changed in all panicle sizes in response to stress. MS analysis of salt responsive proteins and 16 other highly abundant proteins of panicle led to the identification of proteins involved in several salt responsive mechanisms which may increase plant adaptation to salt stress including higher constitutive expression level and upregulation of antioxidants, up-regulation of proteins involved in translation, transcription, signal transduction, and ATP generation. To the best of our knowledge, this is the first proteome analysis of plant young panicle which may enhance our understanding of plant molecular responses to salinity. Proteome reference map of rice young panicle is available at http://www.proteome.ir.

Salt stress responses in Populus cathayana Rehder

“…For IPG-DALT electrophoresis, 2-DE was carried out as described by Askari et al [3] and Dooki et al [33] and modified as follows: IPG strips (18 cm, pH 4-7, linear) were rehydrated overnight with 350 ml of rehydration buffer (8 M urea, 0.5% CHAPS, 20 mM DTT, 0.5% (v/v) IPG buffers) in a reswelling tray (Amersham Biosciences) at room temperature. For analytical and preparative gels, 120 mg and 1.3 mg of protein were loaded, respectively.…”

“…The protocol for protein extraction from poplar leaves was based on the published protocols [30][31][32][33] with some modifications. Fresh leaves were ground in liquid N 2 using a mortar and pestle.…”

The Possible overlap between Salinity and Mechanical Challenges in <I>Porteresia Coarctata</I>

“…This indicates a requirement of plasticity to adapt to an enhanced osmotic pressure (Sengupta and Majumder, 2009) Similarly, an increased level of β-dglucanexohydrolase was found in creeping bentgrass (Xu et al, 2010). Cell-wall associated glycine-rich proteins (GRP) reveal both mechanical and defence properties (Dooki et al, 2006;Du et al, 2010). Changes in cytoskeletal as well as plasma-membrane associated proteins with mechanical functions indicate profound alterations in both intracellular and cell wall architecture of plant cells facing the impacts of an osmotic stress.…”

“…Such structural proteins also play a major role in maintaining mechanical strength of plant cells. Proteins having such shared role are actin, tubulin; profilin-an actinbinding protein involved in polymerization and depolymerization of actin filaments; kinesin-a microtubule motor involved in microtubuledependent transport processes, especially during cell cycle and cytokinesis (Pang et al, 2010;Wang et al, 2009;Dooki et al, 2006; Sengupta S et al, (2008) Oryza coarctata plastid NADH dehydrogenase (ndhF) HE577878 Aliscioni S et al, (2012) New Phytol. 193 (2), 304-312 Oryza coarctata plastid ribulose bisphosphate carboxylase HE577876 Aliscioni S et al, (2012) New Phytol.…”

Research on the Rice Proteome: The Contribution of Proteomics Technology in the Creation of Abiotic Stress-Tolerant Plants

“…The reports of Abbasi and Komatsu [1] and Yan et al [57], taken together, show that specific proteins that have enhanced abundances in distinct regions of the rice plant are involved in a coordinated response to salt stress. Dooki et al [12] employed a proteomic approach to further elucidate the mechanism of responses of plants to salt at an early reproductive stage. Rice plants were grown in a culture solution and salt stress was imposed at panicle initiation.…”