Proteomic studies of human and other vertebrate muscle proteins

Shishkin, S. S.; Kovalyov, L. I.; Kovalyova, M. A.

doi:10.1007/s10541-005-0074-7

Cited by 12 publications

(10 citation statements)

References 120 publications

(155 reference statements)

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“…19.192/ profound_bin/WebProFound.exe) search engines were used. Proteins were identified based on the assumptions that peptides were monoisotopic, oxidized at methionine residues and carbamidomethylated at cysteine residues.…”

Section: Methodsmentioning

confidence: 99%

Proteomic Identification of Altered Proteins in Skeletal Muscle During Chronic Potassium Depletion: Implications for Hypokalemic Myopathy

et al. 2006

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Prolonged potassium depletion is a well-known cause of myopathy. The pathophysiology of hypokalemic myopathy, however, remains unclear. We performed a gel-based, differential proteomics study to define altered proteins in skeletal muscles during chronic potassium depletion. BALB/c mice were fed with normal chow (0.36% K+) or K+-depleted (KD) diet (<0.001% K+) for 8 weeks (n = 5 in each group). Left gastrocnemius muscles were surgically removed from each animal. Histopathological examination showed mild-degree infiltration of polymornuclear and mononuclear cells at the interstitium of the KD muscles. Extracted proteins were resolved with two-dimensional electrophoresis (2-DE), and visualized with Coomassie Brilliant Blue R-250 stain. Quantitative intensity analysis revealed 16 up-regulated protein spots in the KD muscles, as compared to the controls. These differentially expressed proteins were subsequently identified by peptide mass fingerprinting and by quadrupole time-of-flight tandem mass spectrometry (Q-TOF MS/MS). Most of the altered proteins induced by chronic potassium depletion were muscle enzymes that play significant roles in several various metabolic pathways. Other up-regulated proteins included myosin-binding protein H, alpha-B Crystallin, and translationally controlled tumor protein (TCTP). These findings may lead to a new roadmap for research on hypokalemic myopathy, to better understanding of the pathophysiology of this medical disease, and to biomarker discovery.

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Section: Methodsmentioning

confidence: 99%

Proteomic Identification of Altered Proteins in Skeletal Muscle During Chronic Potassium Depletion: Implications for Hypokalemic Myopathy

et al. 2006

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“…Skeletal muscle accounts for 40% of mammalian body weight, and at least 25% of the total protein turnover of humans occurs in this tissue [35][36][37]. Specialized proteins, such as actin and myosin, determine the contractile function in muscles.…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

Reversible and irreversible modifications of skeletal muscle proteins in a rat model of acute oxidative stress

Fedorova

Kuleva²,

Hoffmann³

2009

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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Oxidative stress caused by an imbalance of the production of "reactive oxygen species" (ROS) and cellular scavenging systems is known to a play a key role in the development of various diseases and aging processes. Such elevated ROS levels can damage all components of cells, including proteins, lipids and DNA. Here, we study the influence of highly reactive ROS species on skeletal muscle proteins in a rat model of acute oxidative stress caused by X-ray irradiation at different time points. Protein preparations depleted for functional actin by polymerization were separated by gel electrophoresis in two dimensions by applying first non-reductive and then reductive conditions in SDS-PAGE. This diagonal redox SDS-PAGE revealed significant alterations to intra- and inter-molecular disulfide bridges for several proteins, but especially actin, creatine kinase and different isoforms of the myosin light chain. Though the levels of these reversible modifications were increased by oxidative stress, all proteins followed different kinetics. Moreover, a significant degree of protein was irreversibly oxidized (carbonylated), as revealed by western blot analyses performed at different time points.

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“…Soon 2DE was commonly used in various PPGE studies [10]; then it became the methodological basis for the further development of proteomics [8]. It is still widely used in various modifications [1,2,12,13,20,21].…”

Section: Proteomic Technologies Applied To the Studies Of Muscle Protmentioning

confidence: 99%

“…Modern proteomics is a specific biochemical disci pline, the main objects of which include the pro teomes of unicellular and multicellular organisms (from bacteria to mammals, including humans) and the subproteoms of particular cellular structures [1][2][3]. Proteomics also studies various changes in the pro tein composition (proteomic profiles) of cells and tis sues in normal and pathological processes.…”

mentioning

confidence: 99%

The application of proteomic technologies for the analysis of muscle proteins of farm animals used in the meat industry (Review)

Shishkin

Ковалев

Kovaleva

et al. 2014

Appl Biochem Microbiol

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The review briefly summarizes the data on the development of proteomic technologies that became actively used in studies of the muscular proteins of farm animals used in the meat industry in 2006-2013. It has been noted that the main research trends are connected with the detection of changes in muscle proteins during post mortem autolysis and the search for species specific and other protein biomarkers. Par ticular publications regarding the development of methods based on proteomic technologies for monitoring the state of muscle proteins are considered. According to the analyzed data, we can conclude that the field is promising for the solution of a number of pressing problems in applied biochemistry.

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Proteomic studies of human and other vertebrate muscle proteins

Cited by 12 publications

References 120 publications

Proteomic Identification of Altered Proteins in Skeletal Muscle During Chronic Potassium Depletion: Implications for Hypokalemic Myopathy

Proteomic Identification of Altered Proteins in Skeletal Muscle During Chronic Potassium Depletion: Implications for Hypokalemic Myopathy

Reversible and irreversible modifications of skeletal muscle proteins in a rat model of acute oxidative stress

The application of proteomic technologies for the analysis of muscle proteins of farm animals used in the meat industry (Review)

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