Proteomic Study of Microsomal Proteins Reveals a Key Role for Arabidopsis Annexin 1 in Mediating Heat Stress-Induced Increase in Intracellular Calcium Levels

Wang, Xu; Ma, Xiaolong; Wang, Hui; Li, Bingjie; Clark, Greg; Guo, Yi; Roux, Stanley J.; Sun, Dan; Tang, Wenqiang

doi:10.1074/mcp.m114.042697

Cited by 71 publications

(52 citation statements)

References 36 publications

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“…The accumulation of plastid-lipid associated protein seems to be a ripening regulatory mechanism at low altitude grown fruits, as this protein is involved in carotenoid storage into fruit chromoplasts and induced upon high light (Rey et al, 2000), as obviously occurs at low altitude. The up-regulation of annexin (Table 1) in low altitude-grown peach fruits is in agreement with the observation that this protein act as sensor for heat-stress responses in plant cells (Wang et al, 2015). Alternatively, annexin could serve as a signal to regulate ripening or to be involved in exocytosis of cell wall-degrading enzymes, acting to sequester Ca 2+ released from the degrading cell wall matrix, as previously proposed in strawberry and kiwifruit (Bianco et al, 2009; Tanou et al, 2015).…”

Section: Resultssupporting

confidence: 89%

Comparative Physiological and Proteomic Analysis Reveal Distinct Regulation of Peach Skin Quality Traits by Altitude

et al. 2016

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The role of environment in fruit physiology has been established; however, knowledge regarding the effect of altitude in fruit quality traits is still lacking. Here, skin tissue quality characters were analyzed in peach fruit (cv. June Gold), harvested in 16 orchards located in low (71.5 m mean), or high (495 m mean) altitutes sites. Data indicated that soluble solids concentration and fruit firmness at commercial harvest stage were unaffected by alitute. Peach grown at high-altitude environment displayed higher levels of pigmentation and specific antioxidant-related activity in their skin at the commercial harvest stage. Skin extracts from distinct developmental stages and growing altitudes exhibited different antioxidant ability against DNA strand-scission. The effects of altitude on skin tissue were further studied using a proteomic approach. Protein expression analysis of the mature fruits depicted altered expression of 42 proteins that are mainly involved in the metabolic pathways of defense, primary metabolism, destination/storage and energy. The majority of these proteins were up-regulated at the low-altitude region. High-altitude environment increased the accumulation of several proteins, including chaperone ClpC, chaperone ClpB, pyruvate dehydrogenase E1, TCP domain class transcription factor, and lipoxygenase. We also discuss the altitude-affected protein variations, taking into account their potential role in peach ripening process. This study provides the first characterization of the peach skin proteome and helps to improve our understanding of peach's response to altitude.

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Section: Resultssupporting

confidence: 89%

Comparative Physiological and Proteomic Analysis Reveal Distinct Regulation of Peach Skin Quality Traits by Altitude

et al. 2016

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“…This increase in cytosolic [Ca 2+ ] is mediated by plasma membrane‐localized CYCLIC NUCLEOTIDE GATED CHANNEL (CNGC) family proteins in Physcomitrella patens and Arabidopsis (Finka et al ; Gao et al ). Another membrane‐localized Ca 2+ channel, ANNEXIN 1 (ANN1), also contributes to the HS‐induced rise in cytosolic [Ca 2+ ], although it is not responsible for the initial HS‐induced influx of Ca 2+ (Wang et al ). Instead, ANN1 may become activated following a CNGC‐induced increase in cytosolic [Ca 2+ ] to help maintain the high [Ca 2+ ] required to stimulate downstream signaling events during HS (Wang et al ).…”

Section: Heat Shock Signaling Mechanismsmentioning

confidence: 99%

“…Another membrane‐localized Ca 2+ channel, ANNEXIN 1 (ANN1), also contributes to the HS‐induced rise in cytosolic [Ca 2+ ], although it is not responsible for the initial HS‐induced influx of Ca 2+ (Wang et al ). Instead, ANN1 may become activated following a CNGC‐induced increase in cytosolic [Ca 2+ ] to help maintain the high [Ca 2+ ] required to stimulate downstream signaling events during HS (Wang et al ).…”

Section: Heat Shock Signaling Mechanismsmentioning

confidence: 99%

Molecular mechanisms governing plant responses to high temperatures

Kang

Ren

et al. 2018

JIPB

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233

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The increased prevalence of high temperatures (HTs) around the world is a major global concern, as they dramatically affect agronomic productivity. Upon HT exposure, plants sense the temperature change and initiate cellular and metabolic responses that enable them to adapt to their new environmental conditions. Decoding the mechanisms by which plants cope with HT will facilitate the development of molecular markers to enable the production of plants with improved thermotolerance. In recent decades, genetic, physiological, molecular, and biochemical studies have revealed a number of vital cellular components and processes involved in thermoresponsive growth and the acquisition of thermotolerance in plants. This review summarizes the major mechanisms involved in plant HT responses, with a special focus on recent discoveries related to plant thermosensing, heat stress signaling, and HT-regulated gene expression networks that promote plant adaptation to elevated environmental temperatures.

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“…To the best of our knowledge, the response of ann2 knockout mutants to WD has not been studied. Wang et al (2015) described functional redundancy between ANN1 and ANN2 in heat-mediated calcium signaling. ANN2 also may be involved in regulating stomatal movements.…”

Section: Morphophysiological and Molecular Responses To Wd Are Closelmentioning

confidence: 99%

“…We also identified three accessions (An-1, Est-1, and Ga-0) that displayed minimal reductions in shoot size under WD conditions; thus, they can be considered as WD tolerant. Besseau et al, 2007;Székely et al, 2008;Harb et al, 2010;Colebrook et al, 2014), response to sugar starvation (N-MYC DOWNREGULATED-LIKE1 [NDL1] and CYTOCHROME P450 [P450]; Mudgil et al, 2009Mudgil et al, , 2013Hummel et al, 2010) Li et al, 2008;Alexandersson et al, 2010;Jaradat et al, 2013), and calcium fluxes (ANN1 and ANN2; Cantero et al, 2006;Konopka-Postupolska et al, 2009;Wang et al, 2015). Reference genes for quantitative PCR (qPCR) analyses were chosen from the set of superior genes for transcript normalization proposed by Czechowski et al (2005), and four genes (PROTEIN PHOSPHATASE2A SUBUNIT A3, PEROXIN4 [PEX4], UBIQUITIN-PROTEIN LIGASE7, and YELLOW-LEAF-SPECIFIC GENE8) were finally selected for normalization of transcript levels.…”

Section: Natural Variation Of Responses Among Arabidopsis Accessions mentioning

confidence: 99%

Stress-Related Gene Expression Reflects Morphophysiological Responses to Water Deficit

et al. 2017

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(J.H.).Acclimation to water deficit (WD) enables plants to maintain growth under unfavorable environmental conditions, although the mechanisms are not completely understood. In this study, the natural variation of long-term acclimation to moderate and severe soil WD was investigated in 18 Arabidopsis (Arabidopsis thaliana) accessions using PHENOPSIS, an automated phenotyping platform. Soil water content was adjusted at an early stage of plant development and maintained at a constant level until reproductive age was achieved. The accessions were selected based on the expression levels of ANNEXIN1, a drought-related marker. Severe WD conditions had a greater effect on most of the measured morphophysiological traits than moderate WD conditions. Multivariate analyses indicated that trait responses associated with plant size and water management drove most of the variation. Accessions with similar responses at these two levels were grouped in clusters that displayed different response strategies to WD. The expression levels of selected stress-response genes revealed large natural variation under WD conditions. Responses of morphophysiological traits, such as projected rosette area, transpiration rate, and rosette water content, were correlated with changes in the expression of stress-related genes, such as NINE-CIS-EPOXYCAROTENOID DIOXYGENASE3 and N-MYC DOWNREGULATED-LIKE1 (NDL1), in response to WD. Interestingly, the morphophysiological acclimation response to WD also was reflected in the gene expression levels (most notably those of NDL1, CHALCONE SYNTHASE, and MYB DOMAIN PROTEIN44) in plants cultivated under well-watered conditions. Our results may lead to the development of biomarkers and predictors of plant morphophysiological responses based on gene expression patterns.

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Proteomic Study of Microsomal Proteins Reveals a Key Role for Arabidopsis Annexin 1 in Mediating Heat Stress-Induced Increase in Intracellular Calcium Levels

Cited by 71 publications

References 36 publications

Comparative Physiological and Proteomic Analysis Reveal Distinct Regulation of Peach Skin Quality Traits by Altitude

Comparative Physiological and Proteomic Analysis Reveal Distinct Regulation of Peach Skin Quality Traits by Altitude

Molecular mechanisms governing plant responses to high temperatures

Stress-Related Gene Expression Reflects Morphophysiological Responses to Water Deficit

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