Proteomics—A Powerful Tool for Understanding Saline Stress Response in Germinating Seed

Sarkar, Anup Kumar; Sadhukhan, Sanjoy

doi:10.1007/978-3-030-95365-2_24

Cited by 4 publications

(5 citation statements)

References 238 publications

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“…To our knowledge, little research has been conducted on the accumulation of fatty acids and other volatiles during the germination of crop plants. Physiological shifting, such as the alteration of dry seed to imbibed seed or germinating seed, can impact the proprietary enzymes involved in the biochemical production of secondary metabolites or alter the associated pathways, diminishing their stability and contribution (Gomez-Maqueo et al, 2020;El-Maarouf-Bouteau, 2022;Sarkar and Sadhukhan, 2022). Various alkanes were also revealed in the present study during seed germination and post-germination events of 'Bullet' chilli.…”

Section: Results Results Resultsmentioning

confidence: 55%

“…The seed germination stage is one of the essential developmental stages of plant life during which numerical interactions of biochemical molecules take place. The proper germination of a seed necessitates a systematic relationship between the embryo, seed coat, and intermediate endosperm (Sarkar and Sadhukhan, 2022). In the context of crop production, achieving optimal seed germination and successful seedling establishment is a highly sought-after characteristic by farmers, as it contributes to the long-term viability and financial gains of their agricultural endeavors.…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

“…The moisture content of the seed is a significant regulator of biochemical processes, and it determines the nature and emission kinetics of volatile substances produced by seeds. The germination and growth processes of seeds often encompass a range of metabolic alterations, including modifications in the nutritional composition of seeds, particularly in terms of proteins and carbohydrates (Sarkar and Sadhukhan, 2022). These changes serve to furnish the seeds with the necessary energy for successful germination and subsequent growth.…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

“…Some cultivars are significantly affected by biotic and abiotic stresses throughout their lifecycle, most significantly during seed germination. Germination is associated with systematic and synchronized biochemical and metabolic processes (Sarkar and Sadhukhan, 2022). Such compounds can either induce or suppress seed dormancy due to the influence of abiotic influences on organic components present in seeds (Motsa et al, 2017).…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

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Rapid GC-MS based metabolic profiling of Capsicum annuum L. seeds at different phases of germination

SARKAR,

ORAON,

GHOSH

et al. 2024

Not Sci Biol

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Seed germination is the most critical stage of the life cycle of a plant. Metabolic regulation is significant during the course of seed germination for the establishment of seedlings. Upon imbibition, the dry and fully developed seeds undergo the release of various organic molecules, such as low molecular weight carbonyl compounds in the form of gases and volatiles, as well as water-soluble organic components like enzymes and polysaccharides. Volatile organic compounds may impart both positive and negative influences on seed germination. A metabolite profiling approach based on gas chromatography−mass spectrometry (GC-MS) was used to investigate time-dependent metabolic changes during the germination of Capsicum annuum L. cv. ‘Bullet’. This study aimed to identify bioactive compounds from the methanolic extract of dry, imbibed, germinating seeds and young seedlings of the ‘Bullet’ cultivar of C. annuum L. by GC-MS. A total of 56 distinct categories of compounds were identified in dry seeds, while fully absorbed seeds contained 53 compounds. In the germinating seed, 52 compounds were identified. With regard to immature seedlings, a total of 28 compounds were identified. The analysis revealed that each stage has a unique bio-constituent; only five compounds were detected in all extracts.

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Section: Results Results Resultsmentioning

confidence: 55%

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Rapid GC-MS based metabolic profiling of Capsicum annuum L. seeds at different phases of germination

SARKAR,

ORAON,

GHOSH

et al. 2024

Not Sci Biol

View full text Add to dashboard Cite

show abstract

“…The term ‘proteome’ was introduced in 1995 by Australian scientists Marc Wilkins and Keith Williams (Pandey & Mann, 2000). Proteome research was conducted to explore the expression and functional patterns of all proteins in an organism, including the identification of protein expression, modified forms, structural differences, functional changes and protein–protein interactions (Sarkar & Sadhukhan, 2022). For instance, Lu et al.…”

Section: Discussionmentioning

confidence: 99%

Intercropping and appropriate nitrogen application control faba bean Fusarium wilt by improving physiological and biochemical resistance and protein expression of faba bean

Zheng,

Zhang,

Guo

et al. 2023

Plant Pathology

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This study set up four nitrogen application levels (0, 45, 90 and 135 kg/ha) and two planting systems (faba bean monocropping and faba bean and wheat intercropping) to investigate the incidence of faba bean Fusarium wilt under different treatments and determine the resistance enzyme activities and gene expression, protein expression, and other indexes of faba bean plants. At all N levels, faba bean and wheat intercropping controlled faba bean Fusarium wilt by decreasing the content of hydrogen peroxide and superoxide anion in faba bean roots, increasing the enzyme activity and gene expression of the superoxide dismutase and the gene expression of pathogenesis‐related protein 1 (VfPR1), VfPR2, VfPR5 and VfPR10 disease resistance proteins in the roots, with the most significant effects at the N2 level (90 kg/ha). Further investigation of the impact of intercropping on faba bean roots using the N2 treatment showed that faba bean–wheat intercropping upregulated 288 proteins and downregulated 179 proteins compared with monocropping, and the functions of the upregulated proteins were mainly related to energy metabolism, antioxidant enzymes, stress and DNA repair. GO functional analysis showed that the upregulated proteins were mainly focused on the involvement in amide biological processes. KEGG enrichment analysis revealed that faba bean–wheat intercropping upregulated proteins involved in glutathione metabolism and ascorbic acid metabolism. In summary, at the N2 level, faba bean–wheat intercropping effectively mitigated root oxidative stress by enhancing antioxidant and disease resistance mechanisms in faba bean roots.

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Climate Change on Seeds Physiology

Roy,

Biswakarma,

Ghosh

et al. 2024

Advances in Global Change Research

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Proteomics—A Powerful Tool for Understanding Saline Stress Response in Germinating Seed

Cited by 4 publications

References 238 publications

Rapid GC-MS based metabolic profiling of Capsicum annuum L. seeds at different phases of germination

Rapid GC-MS based metabolic profiling of Capsicum annuum L. seeds at different phases of germination

Intercropping and appropriate nitrogen application control faba bean Fusarium wilt by improving physiological and biochemical resistance and protein expression of faba bean

Climate Change on Seeds Physiology

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