Proteomics analysis reveals a dynamic diurnal pattern of photosynthesis-related pathways in maize leaves

Abstract

Plant leaves exhibit differentiated patterns of photosynthesis rates under diurnal light regulation. Maize leaves show a single-peak pattern without photoinhibition at midday when the light intensity is maximized. This mechanism contributes to highly efficient photosynthesis in maize leaves. To understand the molecular basis of this process, an isobaric tag for relative and absolute quantitation (iTRAQ)-based proteomics analysis was performed to reveal the dynamic pattern of proteins related to photosynthetic reactions. Steady, single-peak and double-peak protein expression patterns were discovered in maize leaves, and antenna proteins in these leaves displayed a steady pattern. In contrast, the photosystem, carbon fixation and citrate pathways were highly controlled by diurnal light intensity. Most enzymes in the limiting steps of these pathways were major sites of regulation. Thus, maize leaves optimize photosynthesis and carbon fixation outside of light harvesting to adapt to the changes in diurnal light intensity at the protein level.

Diurnal metabolic regulation of isoflavones and soyasaponins in soybean roots

“…The GO terms associated with ion transport were represented in pattern 6 and had increased expression in the dawn and noon, which consistent with the GO enrichment analysis on diurnal transcripts in rice leaves (Xu et al, 2011). As observed in maize leaves (Feng et al, 2017), the GO terms associated with carbon metabolism and DNA/RNA processing were induced from dawn to dusk. The GO terms linked with plant hormone signal transduction were highlighted from midnight to dawn (Table 2).…”

Ecophysiological Responses of Three Tree Species to a High-Altitude Environment in the Southeastern Tibetan Plateau

“…During the mid-day depression in P n for P. cathayana and S. longistamina, both g s and C i decrease, so stomatal limitation of photosynthesis may explain the mid-day depression in these species. The temperature at noon reaches its peak so that these two species close stomata to avoid water loss and reduce CO 2 absorption [46]. In contrast, the mid-day depression of P n for U. pumila occurs when C i changes in the opposite direction to g s , suggesting that the mid-day depression is primarily attributable to non-stomatal factors.…”

Physiological and Proteomic Analysis Revealed the Response Mechanisms of two Different Grought-resistant Maize Varieties

“…To better understand these changes, isobaric tags for relative and absolute quanti cation (iTRAQ) can identify more numerous proteins and provides more reliable quantitative information than 2-DE analysis [18]. To date, proteomic studies on drought stress were mainly focused on maize inbred lines [19][20][21]. The combination of iTRAQbased quantitative proteomics and detailed physiological analysis to study the response mechanism under drought stress remains incipient.…”