Proteomics and transcriptomics investigation on longissimus muscles in Large White and Casertana pig breeds

Abstract

Consumer complaints against the blandness of modern lean meat and the frequent reference to the more strongly flavored meat that was available years ago have prompted reconsideration of high fatdepositing typical pig breeds. Casertana and Large White pig breeds are characterized by a different tendency toward fat accumulation as they exhibit opposite genetic and physiological traits with respect to the energy metabolism. These physiological differences were investigated in longissimus lumborum muscles through proteomics (2-DE, MS/MS) and microarray approaches. Data were analyzed for pathway and network analyses, as well as GO term enrichment of biological functions. As a result, Casertana showed a greater amount of proteins involved in glycolitic metabolism and mainly rely on fast-mobilizable energy sources. Large White overexpressed cell cycle and skeletal muscle growth related genes. Metabolic behavior and other implications are discussed.

Protein Expression Landscape Defines the Differentiation Potential Specificity of Adipogenic and Myogenic Precursors in the Skeletal Muscle

“…Almost half of total human meat consumption in the world was provided by the pig industry. , Skeletal muscle, accounting for 20% ∼ 50% of body weight, is the primary meat production tissue of pigs . Intensive genetic selection for lean growth has dramatically reduced the intramuscular fat (IMF) content, which impairs the eating quality of meat. , IMF, mainly located between muscle fiber bundles, is a polygenic trait in livestock species. , In order to increase IMF content, proteomics and transcriptomics were widely used in recent years to reveal the key markers associated with the mechanism(s) responsible for differential IMF deposition between high- and low-IMF breeds of livestock. − However, few studies were conducted to evaluate adipogenic and myogenic differentiation potential of precursor cells in skeletal muscle to illustrate the underlying mechanisms of IMF deposition.…”