Proteomics approach for identifying osmotic-stress-related proteins in soybean roots

Changes in the root proteome of Triticosecale grains germinating under osmotic stress

“…The amount of this enzyme significantly decreased in the sample treated with moderate osmotic stress. A similar effect was also reported in other proteomic (Yan et al 2005;Toorchi et al 2009;Mohammadi et al 2012b) and transcriptomic (Espartero et al 1994) studies. This synthetase is probably involved in the control of gene expression under osmotic stress conditions due to its ability to methylate nucleic acids and, therefore, may constitute an interesting target for genetic engineering of plant resistance to this stress (Mohammadi et al 2012b).…”

Germinação e desenvolvimento inicial de plântulas de soja convencional e sua derivada transgênica RR em condições de estresse salino

“…Para as plantas com maior expansão do sistema radicular, há tendência de serem mais resistentes ao efeito do estresse hídrico, uma vez que a regulação da expansão foliar durante o período de exposição ao estresse osmótico ocorre em função da quantidade de água presente nas raízes (TOORCHI et al, 2009) ), em função dos níveis de NaCl. ** e * significativo a 1% e a 5%, respectivamente, pelo teste "t".…”

Proteomics Approach for Identifying Abiotic Stress Responsive Proteins in Soybean

“…Oxidative stress and programmed cell death caused by cysteine proteases have been shown to accompany many environmental stresses (Prasad et al, 1994). In the case of drought stress, up-regulation of reactive oxygen species (ROS) scavengers such as superoxide dismutase (SOD) was reported in soybean seedlings (Toorchi et al, 2009) and in rice (Ali & Komatsu, 2006). Proteomic investigations on soybean root revealed the accumulation of dehydrin and ferritin under drought stress (Alam et al, 2010b).…”

“…Induction of ferritins by excess iron, water stress and abscisic acid has been documented at both transcript and protein level (Ravet et al, 2009). The proteome analysis of two-day-old soybean seedlings subjected to drought stress by withholding of water for two days revealed a variety of responsive proteins involved in metabolism, disease/defense and energy including protease inhibitors (Toorchi et al, 2009). The up-regulation of protease inhibitors in soybean seedling induced by drought stress indicate their defense response to drought stress; this up-regulation also occurs in response to other abiotic stresses, such as cold or salinity (Pernas et al, 2000).…”

“…Down-regulation of this enzyme under drought is consistent with the inhibition of photosynthetic activity as a general feature of abiotic stresses. A proteomic study in soybean revealed that levels of caffeoyl-CoA Omethyltransferase are significantly decreased at the seedling stage when water is withheld for two days (Toorchi et al, 2009). Down-regulation of this protein correlates with the possibility of root elongation under drought stress, and helps the plant to continue root growth and to delay root lignification.…”