Proteomics for Drug Resistance on the Food Chain? Multidrug-Resistant<i>Escherichia coli</i>Proteomes from Slaughtered Pigs

Ramos, Sónia; Silva, Nuno; Hébraud, Michel; Santos, Hugo M.; Nunes-Miranda, Júlio D.; Pinto, Luís; Pereira, José Eduardo; Capelo, José Luís; Poeta, Patrícia; Igrejas, Gilberto

doi:10.1089/omi.2016.0044

Cited by 11 publications

(9 citation statements)

References 54 publications

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“…The LuxS/AI-2 QS system plays a key role in antibiotic resistance in bacteria. The LuxS/AI-2 system controls the expression of a variety of genes and then regulates the cellular activities of bacteria to adapt to different environments (97,(103)(104)(105). Since QS controls the expression of many virulence factors and drug-resistant genes in bacteria, any process that blocks QS signaling molecules or receptor-recognizing signaling molecules can attenuate the virulence and resistance gene expression of bacterial QS-dependent genes (106)(107)(108)(109).…”

Section: Resultsmentioning

confidence: 99%

Regulatory Mechanisms of the LuxS/AI-2 System and Bacterial Resistance

Wang

Liu

Grenier

et al. 2019

Antimicrob Agents Chemother

102

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The quorum-sensing (QS) system is an intercellular cell-cell communication mechanism that controls the expression of genes involved in a variety of cellular processes and that plays critical roles in the adaption and survival of bacteria in their environment. The LuxS/AI-2 QS system, which uses AI-2 (autoinducer-2) as a signal molecule, has been identified in both Gram-negative and Gram-positive bacteria. As one of the important global regulatory networks in bacteria, it responds to fluctuations in the numbers of bacteria and regulates the expression of a number of genes, thus affecting cell behavior. We summarize here the known relationships between the LuxS/AI-2 system and drug resistance, discuss the inhibition of LuxS/AI-2 system as an approach to prevent bacterial resistance, and present new strategies for the treatment of drug-resistant pathogens.

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Section: Resultsmentioning

confidence: 99%

Regulatory Mechanisms of the LuxS/AI-2 System and Bacterial Resistance

Wang

Liu

Grenier

et al. 2019

Antimicrob Agents Chemother

102

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“…Proteomics is a rapidly emerging specialty of postgenomics medicine and one of the key focus areas of environmental health research agenda (Hayes et al, 2016;Murthy et al, 2016;Ramos et al, 2016). Relatively little research exists on the long-term effects of cigarette smoke on lung cells using proteomics biotechnology.…”

Section: Discussionmentioning

confidence: 99%

Chronic Cigarette Smoke Mediated Global Changes in Lung Mucoepidermoid Cells: A Phosphoproteomic Analysis

Solanki

Advani

Khan

et al. 2017

OMICS: A Journal of Integrative Biology

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Proteomics analysis of chronic cigarette smoke exposure is a rapidly emerging postgenomics research field. While smoking is a major cause of lung cancer, functional studies using proteomics approaches could enrich our mechanistic understanding of the elusive lung cancer global molecular signaling and cigarette smoke relationship. We report in this study on a stable isotope labeling by amino acids in cell culture-based quantitative phosphoproteomic analysis of a human lung mucoepidermoid carcinoma cell line, H292 cells, chronically exposed to cigarette smoke. Using high resolution Orbitrap Velos mass spectrometer, we identified the hyperphosphorylation of 493 sites, which corresponds to 341 proteins and 195 hypophosphorylated sites, mapping to 142 proteins upon smoke exposure (2.0-fold change). We report differential phosphorylation of multiple kinases, including PAK6, EPHA4, LYN, mitogen-activated protein kinase, and phosphatases, including TMEM55B, PTPN14, TIGAR, among others, in response to chronic cigarette smoke exposure. Bioinformatics analysis revealed that the molecules differentially phosphorylated upon chronic exposure of cigarette smoke are associated with PI3K/AKT/mTOR and CDC42-PAK signaling pathways. These signaling networks are involved in multiple cellular processes, including cell polarity, cytoskeletal remodeling, cellular migration, protein synthesis, autophagy, and apoptosis. The present study contributes to emerging proteomics insights on cigarette smoke mediated global signaling in lung cells, which in turn may aid in development of precision medicine therapeutics and postgenomics biomarkers.

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“…Beyond surveillance studies, our research group has investigated AMR mechanisms by characterizing a range of resistant strains of interest through proteomic approaches in MRSA (Monteiro et al, 2012, 2015) and other bacterial species (Pinto et al, 2010; Radhouani et al, 2010, 2012; Correia et al, 2014, 2016; Goncalves et al, 2014; Ramos et al, 2015, 2016; Monteiro et al, 2016). However, these proteomic studies, and most AMR research in general, have focused on bacteria growing in planktonic cultures and hence overlooked biofilm-specific AMR mechanisms.…”

Section: Preliminary and Expected Resultsmentioning

confidence: 99%

Planning a One Health Case Study to Evaluate Methicillin Resistant Staphylococcus aureus and Its Economic Burden in Portugal

et al. 2018

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Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most important multidrug-resistant nosocomial pathogens worldwide with infections leading to high rates of morbidity and mortality, a significant burden to human and veterinary clinical practices. The ability of S. aureus colonies to form biofilms on biotic and abiotic surfaces contributes further to its high antimicrobial resistance (AMR) rates and persistence in both host and non-host environments, adding a major ecological dimension to the problem. While there is a lot of information on MRSA prevalence in humans, data about MRSA in animal populations is scarce, incomplete and dispersed. This project is an attempt to evaluate the current epidemiological status of MRSA in Portugal by making a single case study from a One Health perspective. We aim to determine the prevalence of MRSA in anthropogenic sources liable to contaminate different animal habitats. The results obtained will be compiled with existing data on antibiotic resistant staphylococci from Portugal in a user-friendly database, to generate a geographically detailed epidemiological output for surveillance of AMR in MRSA. To achieve this, we will first characterize AMR and genetic lineages of MRSA circulating in northern Portugal in hospital wastewaters, farms near hospitals, farm animals that contact with humans, and wild animals. This will indicate the extent of the AMR problem in the context of local and regional human-animal-environment interactions. MRSA strains will then be tested for their ability to form biofilms. The proteomes of the strains will be compared to better elucidate their AMR mechanisms. Proteomics data will be integrated with the genomic and transcriptomic data obtained. The vast amount of information expected from this omics approach will improve our understanding of AMR in MRSA biofilms, and help us identify new vaccine candidates and biomarkers for early diagnosis and innovative therapeutic strategies to tackle MRSA biofilm-associated infections and potentially other AMR superbugs.

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Proteomics for Drug Resistance on the Food Chain? Multidrug-ResistantEscherichia coliProteomes from Slaughtered Pigs

Cited by 11 publications

References 54 publications

Regulatory Mechanisms of the LuxS/AI-2 System and Bacterial Resistance

Regulatory Mechanisms of the LuxS/AI-2 System and Bacterial Resistance

Chronic Cigarette Smoke Mediated Global Changes in Lung Mucoepidermoid Cells: A Phosphoproteomic Analysis

Planning a One Health Case Study to Evaluate Methicillin Resistant Staphylococcus aureus and Its Economic Burden in Portugal

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