Proteomics in medical microbiology

Cash, Phillip

doi:10.1002/(sici)1522-2683(20000401)21:6<1187::aid-elps1187>3.0.co;2-f

Cited by 59 publications

(14 citation statements)

References 154 publications

(99 reference statements)

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“…In order to obtain more accurate proteome measurements, quantitative proteomic methods, such as stable isotope labelling-based isotope-coded affinity tags (ICAT), isobaric tag for relative and absolute quantification (iTRAQ) (Yan et al, 2008) or label-free comparative quantitative proteomics, need to be employed (Haqqani et al, 2008). Proteomics has been extensively used to explore microbial metabolism, differentiation and relationship with environments (for recent reviews see Cash, 2000;Fraser & Rappuoli, 2005;Norbeck et al, 2006;Graham et al, 2007;Zhao & Poh, 2008;Lacerda & Reardon, 2009). …”

Section: Proteomicsmentioning

confidence: 99%

Integrating multiple ‘omics’ analysis for microbial biology: application and methodologies

Zhang

Li²,

Nie³

2010

Microbiology

416

241

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Recent advances in various ‘omics’ technologies enable quantitative monitoring of the abundance of various biological molecules in a high-throughput manner, and thus allow determination of their variation between different biological states on a genomic scale. Several popular ‘omics’ platforms that have been used in microbial systems biology include transcriptomics, which measures mRNA transcript levels; proteomics, which quantifies protein abundance; metabolomics, which determines abundance of small cellular metabolites; interactomics, which resolves the whole set of molecular interactions in cells; and fluxomics, which establishes dynamic changes of molecules within a cell over time. However, no single ‘omics’ analysis can fully unravel the complexities of fundamental microbial biology. Therefore, integration of multiple layers of information, the multi-‘omics’ approach, is required to acquire a precise picture of living micro-organisms. In spite of this being a challenging task, some attempts have been made recently to integrate heterogeneous ‘omics’ datasets in various microbial systems and the results have demonstrated that the multi-‘omics’ approach is a powerful tool for understanding the functional principles and dynamics of total cellular systems. This article reviews some basic concepts of various experimental ‘omics’ approaches, recent application of the integrated ‘omics’ for exploring metabolic and regulatory mechanisms in microbes, and advances in computational and statistical methodologies associated with integrated ‘omics’ analyses. Online databases and bioinformatic infrastructure available for integrated ‘omics’ analyses are also briefly discussed.

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Section: Proteomicsmentioning

confidence: 99%

Integrating multiple ‘omics’ analysis for microbial biology: application and methodologies

Zhang

Li²,

Nie³

2010

Microbiology

416

241

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“…39 Advances in relevant proteomic techniques such as 2-dimensional gel electrophoresis and mass spectroscopy 68 have led to the identification of several new proteins involved in mastitis. Progress in microbial proteomics has been achieved with the availability of whole genome sequences for a number of bacterial groups, 13 including proteome profiles of mastitis-causing pathogens, which, combined with newly available information on toxins, enzymes, and metabolites produced in the udder, could lead to their identification in milk. 76 Proteomic studies performed for several mastitis pathogens have led to information on protein expression pattern, which can be applied to the discovery of new therapeutic targets 46 (e.g., bacterial immunogenic proteins for vaccines) and new diagnostic biomarkers.…”

Section: Future Trendsmentioning

confidence: 99%

Technological advances in bovine mastitis diagnosis

2015

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Abstract. Bovine mastitis is an economic burden for dairy farmers and preventive control measures are crucial for the sustainability of any dairy business. The identification of etiological agents is necessary in controlling the disease, reducing risk of chronic infections and targeting antimicrobial therapy. The suitability of a detection method for routine diagnosis depends on several factors, including specificity, sensitivity, cost, time in producing results, and suitability for large-scale sampling of milk. This article focuses on current methodologies for identification of mastitis pathogens and for detection of inflammation, as well as the advantages and disadvantages of different methods. Emerging technologies, such as transcriptome and proteome analyses and nano-and microfabrication of portable devices, offer promising, sensitive methods for advanced detection of mastitis pathogens and biomarkers of inflammation. The demand for alternative, fast, and reliable diagnostic procedures is rising as farms become bigger. Several examples of technological and scientific advances are summarized which have given rise to more sensitive, reliable and faster diagnostic results.

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“…For example, the synthesis of the bacterial heat shock proteins is induced during Brucella abortus infection of bovine and murine macrophages. The changes in the patterns of in vivo gene expression of pathogens have been revealed using 2-DE combined with metabolic radiolabelling or immunoblotting (Cash, 2000). The expression pattern of proteins at higher levels in intracellular bacteria is generally similar to those induced in bacteria in response to stress conditions including extreme acidity, oxygen and high temperature.…”

Section: Evaluation Of Proteins Involved In the Toxic Responsementioning

confidence: 99%

“…Bacteria recognize drugs and antibiotics as toxic materials and produce several proteins in response to these stresses. High resolution 2-DE has been employed to investigate the betalactam antibiotics-resistance in Pseudomonas aeruginosa and S. pneumoniae (Cash, 2000). Identification of those proteins involved in drug resistance will lead to improvements in future antimicrobials.…”

Section: Evaluation Of Proteins Involved In the Toxic Responsementioning

confidence: 99%