Proteomics in Seaweeds: Ecological Interpretations

Contreras–Porcia, Loretto; López-Cristoffanini, Camilo

doi:10.5772/37965

Cited by 5 publications

(5 citation statements)

References 98 publications

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“…In plants, PRX transcripts increase in response to different abiotic stresses such as salinity, drought, and heavy metals (Wood et al, 2003;Dietz, 2011). Their expression in algae has been poorly studied, although some studies indicate they are regulated by light, oxygen, copper, desiccation and redox state (Goyer et al, 2002;Contreras-Porcia et al, 2011a, 2001bLovazzano et al, 2013; see section 3 in Contreras-Porcia & López-Cristoffanini, 2012). In vascular plants, several PRXs have been described based on their catalytic mechanisms and subcellular localization (Baier & Dietz, 1997).…”

Section: Discussionmentioning

confidence: 99%

Differential gene expression in Pyropia columbina (Bangiales, Rhodophyta) under natural hydration and desiccation conditions

Contreras–Porcia¹,

López-Cristoffanini²,

Lovazzano³

et al. 2013

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ABSTRACT. In rocky shores, desiccation is triggered by daily tide changes, and experimental evidence suggests that local distribution of algal species across the intertidal rocky zone is related to their capacity to tolerate desiccation. In this context, the permanence of Pyropia columbina in the high intertidal rocky zone is explained by its exceptional physiological tolerance to desiccation. This study explored the metabolic pathways involved in tolerance to desiccation in the Chilean P. columbina, by characterizing its transcriptome under contrasting conditions of hydration. We obtained 1,410 ESTs from two subtracted cDNA libraries in naturally hydrated and desiccated fronds. Results indicate that transcriptome from both libraries contain transcripts from diverse metabolic pathways related to tolerance. Among the transcripts differentially expressed, 15% appears involved in protein synthesis, processing and degradation, 14.4% are related to photosynthesis and chloroplast, 13.1% to respiration and mitochondrial function (NADH dehydrogenase and cytochrome c oxidase proteins), 10.6% to cell wall metabolism, and 7.5% are involved in antioxidant activity, chaperone and defense factors (catalase, thioredoxin, heat shock proteins, cytochrome P450). Both libraries highlight the presence of genes/proteins never described before in algae. This information provides the first molecular work regarding desiccation tolerance in P. columbina, and helps, to some extent, explaining the classical patterns of ecological distribution described for algae across the intertidal zone. Keywords: Pyropia, desiccation stress, ESTs, seaweeds, transcriptomics, proteins. Expresión diferencial de genes en Pyropia columbina (Bangiales, Rhodophyta)bajo hidratación y desecación natural RESUMEN. En zonas rocosas costeras, la desecación es gatillada por cambios diarios en los niveles de marea, y la evidencia experimental indica que la distribución de las algas en la zona intermareal está relacionada con su capacidad para tolerar la desecación. En este contexto, la presencia de Pyropia columbina en la zona alta del intermareal se explica por su excepcional tolerancia fisiológica a la desecación. Este estudio explora las vías metabólicas involucradas en la tolerancia a la desecación en P. columbina, a través de la caracterización de su transcriptoma bajo condiciones de hidratación contrastantes. Se obtuvo 1,410 ESTs provenientes de dos librerías de substracción de cDNA de frondas naturalmente hidratadas y desecadas. Los transcriptomas de ambas librerías contienen transcritos de diversas rutas metabólicas relacionadas a la tolerancia. Entre los transcritos expresados 15% están involucrados en la síntesis de proteínas, su procesamiento y degradación, 14,4% asociados a fotosíntesis y cloroplasto, 13,1% a respiración y función mitocondrial, 10,6% al metabolismo de la pared celular y 7,5% a la actividad antioxidante, proteínas chaperonas y factores de defensa (catalasa, tiorredoxina, proteínas de shock térmico, citocromo P450). En ambas librerías se de...

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Section: Discussionmentioning

confidence: 99%

Differential gene expression in Pyropia columbina (Bangiales, Rhodophyta) under natural hydration and desiccation conditions

Contreras–Porcia¹,

López-Cristoffanini²,

Lovazzano³

et al. 2013

lajar

View full text Add to dashboard Cite

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“…Therefore, it represents the ideal technological approach for studying eco-physiological responses and adaptability, which usually make an alien species a successful invader (Slattery et al 2012). Nevertheless, proteome analysis has been scarcely applied to bio-ecological studies of the marine environment (Knigge 2015) and especially to macroalgae study (Contreras-Porcia and López-Cristoffanini 2012). Indeed, protein extraction from macroalgae appears to be difficult due to the large amounts of interfering compounds such as phenols, carbohydrates, terpenes, and pigments (Wang et al 2008a(Wang et al , 2008bWu et al 2014;Mohd Rosni et al 2015;Cotas et al 2020) as well as to the significant presence of cell wall anionic polysaccharides (Kraan 2012).…”

Section: Introductionmentioning

confidence: 99%

Comparative evaluation of multiple protein extraction procedures from three species of the genus Caulerpa

et al. 2021

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The aim of this study was to define the simplest and least expensive protocol for total protein extraction for three different macroalgae of the genus Caulerpa (the invasive C. taxifolia and C. cylindracea and the autochthonous C. prolifera). Five multi-step protein extraction procedures, set up for other macroalgal species, were tested. For each of them, different pre-treatment and extraction conditions were simultaneously examined, according to a factorial design, considering the starting material, the solvent-to-biomass ratio, and the incubation temperature. Protein yield in the obtained extracts was estimated with the Bradford method. Further, polyacrylamide gel electrophoresis (SDS-PAGE) was used to resolve proteins, assessing their quality and integrity. Significant differences in protein yield were observed among the extraction protocols and the conditions tested, also in relation to the considered species. Profiles having an acceptable quality were obtained for C. prolifera and C. cylindracea, and from the obtained results, the best method to obtain high yield and quality protein extracts for the two above-mentioned species appears to require the use of a primary TCA/acetone extraction buffer followed by a lysis buffer with NaCl, KCl, urea, Triton, SDS and a protease inhibitor. The best results, in particular, were obtained starting from fresh pulped material with a buffer-to-biomass ratio of 10:1 and an incubation temperature of 4°C. For C. taxifolia, instead, none of the tested protocols produced satisfactory results and further studies will be required.

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“…In addition, 'marine algal-omics' encounters pronounced issues with respect to macroalgae and microalgae. Contreras-Porcia and Lopez-Cristoffanini [170] underlined that protein extraction of algae follows no common protocol and pointed out that it is particularly difficult to extract proteins from macroalgae due to their low concentration and the co-extraction of contaminants. In the years to come, the proteomics studies of marine algae are expected to increase by multiple folds taking into account their commercial/industrial implication and the will of many countries to develop their blue economy.…”

Section: Marine Algal-omicsmentioning

confidence: 99%

Challenges and Opportunities in the Present Era of Marine Algal Applications

Beetul¹,

Gopeechund²,

Kaullysing³

et al. 2016

Algae - Organisms for Imminent Biotechnology

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Marine algae are of high importance in their natural habitats and even more now in the world of green technology. The sprouting interest of the scientific community and industries in these organisms is driven by the fast-growing world of modern biotechnology. Genomics, transcriptomics, proteomics, metabolomics and their integration collectively termed here as 'marine algal-omics' have broadened the research horizon in view of enhancing human's life by addressing environmental problems and encouraging novelty in the field of pharmaceuticals among so many more. Their use in the human society dates back to 500 B. C. in China and later across the globe; they are still being used for similar purposes and more today. There is a hiking interest in marine algae and their derivatives-from phycoremediation, food supplements, pharmaceuticals to dyes. Marine algae are currently considered as an emerging panacea for the society. They are being studied in a multitude of arenas. The multi-use of marine algae is enticing and promises to be a boon for industrial applications. Yet, most marine algae face challenges that might variably constrain their commercialisation. This chapter gives an overview of marine algae including all the 'omics' technologies involved in studying marine algae and it explores their multitude applications. It also draws the various successful industries budded around them and presents some of the challenges and opportunities along with future directions.

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Proteomics in Seaweeds: Ecological Interpretations

Cited by 5 publications

References 98 publications

Differential gene expression in Pyropia columbina (Bangiales, Rhodophyta) under natural hydration and desiccation conditions

Differential gene expression in Pyropia columbina (Bangiales, Rhodophyta) under natural hydration and desiccation conditions

Comparative evaluation of multiple protein extraction procedures from three species of the genus Caulerpa

Challenges and Opportunities in the Present Era of Marine Algal Applications

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