Proteomics of a new esophageal cancer cell line established from Persian patient

Moghanibashi, Mehdi; Jazii, Ferdous Rastgar; Soheili, Zahra‐Soheila; Zare, Maryam; Karkhane, Ali Asghar; Parivar, Kazem; Mohamadynejad, Parisa

doi:10.1016/j.gene.2012.03.038

Cited by 29 publications

(19 citation statements)

References 53 publications

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“…Previous studies have demonstrated that expression changes of genes and proteins are associated with tumor progression (6,10–13). The mutant genes are transcribed and translated into proteins, and the expression levels of proteins dictate the tumor phenotype and regulate its biological behavior.…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis indicates the importance of TPM3 in esophageal squamous cell carcinoma invasion and metastasis

Gao

Lin

et al. 2017

Molecular Medicine Reports

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Numerous esophageal squamous cell carcinoma (ESCC) patients exhibit tumor recurrence following radical resection. Invasion and metastasis are key factors in poor prognosis following esophagectomy. In the present study, two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry were used to define patterns of protein expression in ESCC tissues at different pathological stages. The expression levels of identified proteins were determined by immunohistochemistry and western blotting. A total of fifteen protein spots with >2-fold differences were observed when comparing results of 2-DE for stage III and stage I ESCC tissue sample. A total of 12 proteins were identified by mass spectrometry analysis and database searches. The results of immunohistochemistry and western blotting demonstrated expression levels of tropomyosin 3 (TPM3) were higher in stage III ESCC tissue compared with stage I (P<0.05). The findings of the present study identified twelve proteins, which are closely associated with ESCC invasion and metastasis, apoptosis and cell signal transduction. Furthermore, the overexpression of TPM3 may be important in ESCC invasion and metastasis.

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Section: Discussionmentioning

confidence: 99%

Proteomic analysis indicates the importance of TPM3 in esophageal squamous cell carcinoma invasion and metastasis

Gao

Lin

et al. 2017

Molecular Medicine Reports

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“…So far, only one paper reports on the association of Anxa4 with ESCC [61]. Comparative proteomics performed between an established ESCC cell prepared from a 69-year-old female with moderately differentiated ESCC and epithelium cell prepared from normal esophageal mucosa at the distant tumor margin following esophagectomy, the results showed that Anxa4 protein level was 2-fold in the former than that in the latter [61]. Although no study to further investigate the function and mechanism of Anxa4 in ESCC, the current work might provide new insight into the relationship between Anxa4 and ESCC.…”

Section: Anxa4 and Esophageal Squamous Cell Carcinomamentioning

confidence: 99%

Annexin A4 and cancer

Wei

Guo

Liu

et al. 2015

Clinica Chimica Acta

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“…Previous studies found that ANXA1 is upregulated in breast cancer [11] , lung cancer [12] , pancreatic cancer [13] , colorectal cancer [3,14] and bladder cancer [15] . By contrast, ANXA1 is downregulated in oral squamous cell carcinoma as well as nasopharyngeal, laryngeal and other head and neck cancer [16][17][18] , oesophageal squamous cell carcinoma [2] and prostate cancer [19] . The dysfunction of ANXA1 is closely related to breast cancer, lung cancer, and pancreatic cancer as well as in other tumour invasion and metastasis.…”

Section: Discussionmentioning

confidence: 98%

“…This abnormal expression is closely related to tumourigenesis, development, invasion and metastasis of human tumours. The expression of ANXA1 in tumours is tissue specific; for instance, a low ANXA1 expression is observed in oesophageal squamous cell carcinoma [2] , whereas a high expression is found in colorectal cancer [3] . The relationship between ANXA1 expression in GC and GC invasion as well as metastasis remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Identification of Annexin A1 protein expression in human gastric adenocarcinoma using proteomics and tissue microarray

Zhang

Li²,

Liu³

et al. 2013

WJG

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AIM:To study the differential expression of Annexin A1 (ANXA1) protein in human gastric adenocarcinoma. This study was also designed to analyze the relationship between ANXA1 expression and the clinicopathological parameters of gastric carcinoma. O after trypsin digestion. Differential protein expressions were quantitatively identified between GAC and NGEC by nanoliter-reverse-phase liquid chromatography-mass/mass spectrometry (nano-RPLC-MS/MS). The expressions of ANXA1 in GAC and NGEC were verified by western blot analysis. The tissue microarray containing the expressed ANXA1 in 75 pairs of gastric carcinoma and paracarcinoma specimens was detected by immunohistochemistry (IHC). The relationship between ANXA1 expression and clinicopathological parametes of gastric carcinoma was analyzed. METHODS: RESULTS:A total of 78 differential proteins were identified. Western blotting revealed that ANXA1 expression was significantly upregulated in GAC (2.17/1, P < 0.01).IHC results showed the correlations between ANXA1 protein expression and the clinicopathological parameters, including invasive depth (T stage), lymph node metastasis (N stage), distant metastasis (M stage) and tumour-lymph node metastasis stage (P < 0.01). However, the correlations between ANXA1 protein expression and the remaining clinicopathological parameters, including sex, age, histological differentiation and the size of tumour were not found (P > 0.05). CONCLUSION:The upregulated ANXA1 expression may be associated with carcinogenesis, progression, invasion and metastasis of GAC. This protein could be considered as a biomarker of clinical prognostic prediction and targeted therapy of GAC.

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Proteomics of a new esophageal cancer cell line established from Persian patient

Cited by 29 publications

References 53 publications

Proteomic analysis indicates the importance of TPM3 in esophageal squamous cell carcinoma invasion and metastasis

Proteomic analysis indicates the importance of TPM3 in esophageal squamous cell carcinoma invasion and metastasis

Annexin A4 and cancer

Identification of Annexin A1 protein expression in human gastric adenocarcinoma using proteomics and tissue microarray

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