Proteomics of epicardial adipose tissue in patients with heart failure

Abstract

Epicardial adipose tissue (EAT) is a metabolically active visceral fat depot closely linked to the pathogenesis of heart failure (HF). But the molecular signatures related to the mechanism of HF have not been systematically explored. Here, we present comprehensive proteomic analysis of EAT in HF patients and non‐HF patients as controls. A total of 771 proteins were identified in liquid chromatography‐tandem mass spectrometry experiments. Amongst them, 17 increased in abundance in HF and seven decreased. They were involved in HF‐related processes including inflammation and oxidative stress response and lipid metabolism. Of these proteins, serine proteinase inhibitor A3 (Serpina3) levels in EAT were highly up‐regulated in HF, with HF/non‐HF ratio of 4.63 (P = .0047). Gene expression of Serpina3 via quantitative polymerase chain reaction was significantly increased in the HF group. ELISA analysis confirmed a significant increase in circulating plasma Serpina3 levels in the HF group (P = .004). In summary, for the first time, we describe that parts of EAT proteome may be reactive and work as modulators of HF. Our profiling provides a comprehensive basis for linking EAT with pathogenesis of HF. Understanding the role of EAT may offer new insights into the treatment of HF.

Multiple Feature Selection Strategies Identified Novel Cardiac Gene Expression Signature for Heart Failure

“…In circulating plasma, the level of SERPINA3 in the HF group was confirmed significant increase by ELISA analysis. These results suggested that SERPINA3 might play an important role in the progression of HF ( Zhao et al, 2020 ). Asakura and Kitakaze (2009) proved that SERPINA3 might become novel diagnostic and therapeutic targets linked to the pathophysiology of HF using seven microarray datasets previously reported.…”

Finding a Needle in a Haystack

“…Several studies have examined MS/MS proteomics to evaluate the disease state of HF ( 9 , 44 , 45 , 46 , 47 ). In a study of ischemic cardiomyopathy (ICM), Yi et al.…”

Mechanism of Hip Arthropathy in Ankylosing Spondylitis: Abnormal Myeloperoxidase

“…Proteins are the end products of gene expression and can better and directly illuminate the processes behind the manifestation of diseases. Comparative proteomics methods are widely used to analyze the relative amounts of proteins in two or more biological samples to screen for biomarker proteins for judgment and prognosis [12][13][14]. Label-free quantitative (LFQ) analysis is one of proteomics methods [12,13].…”

“…Comparative proteomics methods are widely used to analyze the relative amounts of proteins in two or more biological samples to screen for biomarker proteins for judgment and prognosis [12][13][14]. Label-free quantitative (LFQ) analysis is one of proteomics methods [12,13]. Compared to label quantitative analysis, LFQ can avoid the drawbacks of labeling quanti cation techniques: additional sample processing, sample complexity caused by incomplete labeling, di culty in detecting low abundance peptides, limited number of labeled samples, etc [13,15].…”

“…Label-free quantitative (LFQ) analysis is one of proteomics methods [12,13]. Compared to label quantitative analysis, LFQ can avoid the drawbacks of labeling quanti cation techniques: additional sample processing, sample complexity caused by incomplete labeling, di culty in detecting low abundance peptides, limited number of labeled samples, etc [13,15].…”