Proteomics of immune-challenged Drosophila melanogaster larvae hemolymph

Guedes, Sofia; Vitorino, Rui; Domingues, Rosário; Tomer, Kenneth B.; Correia, António; Amado, Francisco; Domingues, Pedro

doi:10.1016/j.bbrc.2004.12.135

Cited by 76 publications

(59 citation statements)

References 41 publications

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“…Cellular stress such as phagocytosis and melanotic encapsulation can induce the generation of reactive oxygen species (ROS) such as superoxide anions, hydrogen peroxide, and hydroxyl radicals that damage nucleic acids, proteins, lipids, and membranes (Müller, 2004;de Morais Guedes et al, 2005). In order to counter these deleterious events, cells use several protective systems that either repair the various types of damage (for instance, by using DNA repair enzymes) or inactivate the ROS (de Morais Guedes et al, 2005).…”

Section: Detoxification Proteinsmentioning

confidence: 99%

“…In order to counter these deleterious events, cells use several protective systems that either repair the various types of damage (for instance, by using DNA repair enzymes) or inactivate the ROS (de Morais Guedes et al, 2005). There are three different kinds of primary antioxidant enzymes that act directly on ROS: superoxide dismutases, catalases, and peroxidases (Corona and Robinson, 2006).…”

Section: Detoxification Proteinsmentioning

confidence: 99%

“…The antioxidant activity of Prx is due to a reduction of hydroperoxide, with thioredoxin as an immediate hydrogen donor (Rodriguez et al, 2000). Perturbation in the expression of detoxification proteins has been found in insect subjected to an immune challenge, exposed to a temperature stress or to viral infections (de Morais Guedes et al, 2005;Lee et al, 2005). Using 2D gel analysis, Nguyen et al(2008) reported an increase in such types of proteins in the aphid Macrosiphum euphorbiae challenged by the parasitoids Aphidius nigripes or A. ervi.…”

Section: Detoxification Proteinsmentioning

confidence: 99%

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Proteome changes in the plasma of Papilio xuthus (Lepidoptera: Papilionidae): effect of parasitization by the endoparasitic wasp Pteromalus puparum (Hymenoptera: Pteromalidae)

Zhu

Yè

Fang

et al. 2009

J. Zhejiang Univ. Sci. B

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Abstract:Although the biochemical dissection of parasitoid-host interactions is becoming well characterized, the molecular knowledge concerning them is minimal. In order to understand the molecular bases of the host immune response to parasitoid attack, we explored the response of Papilio xuthus parasitized by the endoparasitic wasp Pteromalus puparum using proteomic approach. By examining the differential expression of plasma proteins in the parasitized and unparasitized host pupae by two-dimensional (2D) electrophoresis, 16 proteins were found to vary in relation to parasitization compared with unparasitized control samples. All of them were submitted to identification by mass spectrometry coupled with a database search. The modulated proteins were found to fall into the following functional groups: humoral or cellular immunity, detoxification, energy metabolism, and others. This study contributes insights into the molecular mechanism of the relationships between parasitoids and their host insects.

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Section: Detoxification Proteinsmentioning

confidence: 99%

Section: Detoxification Proteinsmentioning

confidence: 99%

Section: Detoxification Proteinsmentioning

confidence: 99%

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Proteome changes in the plasma of Papilio xuthus (Lepidoptera: Papilionidae): effect of parasitization by the endoparasitic wasp Pteromalus puparum (Hymenoptera: Pteromalidae)

Zhu

Yè

Fang

et al. 2009

J. Zhejiang Univ. Sci. B

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“…NF-κB was demonstrated to play major role in the activation of numerous genes involved in the function and development of immune system (Baeuerle andHenkel, 1994, Baldwin, 1996). Chymotrypsin-like serine proteinase is involved in immune defense reactions against bacteria in Drosophila (de Morais Guedes et al, 2005), and innate immune reactions in Fenneropenaeus chinensis (Shi et al, 2008). Alpha 2-macroglobulin (α2-M) is protease inhibitor that can neutralize pathogenic proteases that contribute to pathogen virulence (Lin et al, 2008).…”

Section: Identification Of Differentially Expressed Estsmentioning

confidence: 99%

Screening of genes related to ovarian development in the swimming crab, Portunus trituberculatus, by suppression subtractive hybridization

Song

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The swimming crab, Portunus trituberculatus, is an important marine animal and is widely cultured in China. In the present study, suppression subtractive hybridization was applied to identify the differentially expressed genes in the ovaries of mature and immature P. trituberculatus. One hundred and seventy six expressed sequence tag (ESTs) were identified, of which 100 were down-regulated, and 76 up-regulated. BLAST analysis identified 51 unigenes, of which 27 were down-regulated, and 24 up-regulated. Quantitative real-time reverse transcriptase polymerase chain reaction results indicated that the SSH technique is valuable in screening genes related to ovarian development. Genes identified in this study encoded proteins corresponding to a wide range of functions and included immune response protein, transcription initiation factor, metabolic proteins, chromosome, histone h3, ovarian development-related protein, and vitellogenin. In addition, 64 metabolic pathways were annotated in differentially expressed ESTs by using the Kyoto Encyclopedia of Genes and Genomes pathway. Four annotated pathways (oxidative phosphorylation, carbon metabolism, fatty acid degradation, and protein digestion and absorption) appeared to be involved in ovarian development. In ontology analysis, 5.83% of the cellular process genes in reverse subtraction cDNA library are involved in reproduction, and 5.88% involved in developmental process. In up-regulated genes, myosin II-expressed polehole-like protein; histone h3; ovigerous-hair stripping substance; peritrophin 48; and ovarian development-related protein appeared to be involved in ovarian development. Identification of differentially expressed genes in the mature and immature ovary of the swimming crab provides new insights for further studies on the mechanism underlying ovarian development in this species.

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“…Thus, the increase in quantity seen in this protein band post infection is rather due to the advancement in developmental stages of the larvae. Other possible candidates present in this region could also be the stress proteins that are synthesized in larval hemolymph post infection (29).…”

Section: Noduler Is Up-regulated Upon Infection In Hemolymphmentioning

confidence: 99%

Noduler, A Novel Immune Up-Regulated Protein Mediates Nodulation Response in Insects

Gandhe

John

Nagaraju

2007

The Journal of Immunology

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Insect immune system comprises of both humoral and cellular defenses. Nodulation is one of the major, yet very poorly understood cellular responses against microbial infections in insects. Through screening for novel immune genes from an Indian saturniid silkmoth Antheraea mylitta, we identified a protein up-regulated in hemolymph within minutes upon bacterial challenge. We have shown here, for first time, the involvement of this novel protein in mediating nodulation response against bacteria and hence designated it as Noduler. Noduler possessed a characteristic reeler domain found in several extracellular matrix vertebrate proteins. Noduler was shown in vitro to bind a wide range of bacteria, yeast, and also insect hemocytes. Furthermore, Noduler specifically bound LPS, lipotechoic acid, and β-1, 3 glucan components of microbial cell walls. RNA-interference mediated knock-down of the Noduler resulted in significant reduction in the number of nodules and consequent increase in bacterial load in larval hemolymph. The results suggest that the Noduler is widely conserved and is involved in very early clearance of bacteria by forming nodules of hemocytes and bacterial complexes in insects. The results would promote further studies for understanding of the crucial but hitherto overlooked nodulation mechanism in insects and also provide cues for the study of similar mammalian proteins whose function is not understood.

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Proteomics of immune-challenged Drosophila melanogaster larvae hemolymph

Cited by 76 publications

References 41 publications

Proteome changes in the plasma of Papilio xuthus (Lepidoptera: Papilionidae): effect of parasitization by the endoparasitic wasp Pteromalus puparum (Hymenoptera: Pteromalidae)

Proteome changes in the plasma of Papilio xuthus (Lepidoptera: Papilionidae): effect of parasitization by the endoparasitic wasp Pteromalus puparum (Hymenoptera: Pteromalidae)

Screening of genes related to ovarian development in the swimming crab, Portunus trituberculatus, by suppression subtractive hybridization

Noduler, A Novel Immune Up-Regulated Protein Mediates Nodulation Response in Insects

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