Proteomics: Opportunities and Challenges

Abstract

‘‘Proteomics’’, is the emerging technology leading to high-throughput identification and understanding of proteins. Proteomics is the protein equivalent of genomics and has captured the imagination of biomolecular scientists, worldwide. Because proteome reveals more accurately the dynamic state of a cell, tissue, or organism, much is expected from proteomics to indicate better disease markers for diagnosis and therapy monitoring.
 Proteomics is expected to play a major role in biomedical research, and it will have a significant impact on the development of diagnostics and therapeutics for cancer, heart ailments and infectious diseases, in future. Proteomics research leads to the identification of new protein markers for diagnostic purposes and novel molecular targets for drug discovery. 
 Though the potential is great, many challenges and issues remain to be solved, such as gene expression, peptides, generation of low abundant proteins, analytical tools, drug target discovery and cost. A systematic and efficient analysis of vast genomic and proteomic data sets is a major challenge for researchers, today. Nevertheless, proteomics is the groundwork for constructing and extracting useful comprehension to biomedical research. This review article covers some opportunities and challenges offered by proteomics.

Ultrasensitive Chemically Modified Carbon Paste Sensor for Reliable and Selective Potentiometric Determination of Trace Amounts of Sitagliptin in Real Samples

“…Analytical techniques for analyzing sitagliptin in biological media require an understanding of pharmacological processes like distribution, absorption, elimination and metabolism. Various analysis methods for the quantitation of sitagliptin, such as spectrophotometry, [6][7][8][9] spectrofluorimetry, [10,11] HPLC, [12][13][14][15] electrophoresis [16] and voltammetry. [17] Although many of these introduced techniques may be sensitive and accurate, some drawbacks such as low selectivity, sample pretreatment process, time-consuming procedure, special expertise, and expensive and sophisticated devices limit their use in routine analyses.…”

Novel Stress Indicating RP-HPLC Method Development and Validation for the Simultaneous Estimation of Ertugliflozin and Sitagliptin in Bulk and its Formulation

“…The most of the methods were reported for the separation and estimation of Sitagliptin, metformin and few are only on estimation of Sitagliptin. 18,19,20,21,22,23,24,25 The structures of Ertugliflozin and Sitagliptin showed in Figures 1 and 2.…”

Environmentally friendly protocol for the determination of sitagliptin phosphate in pharmaceutical preparations and biological fluids using l‐tyrosine as a fluorescence probe

“…A review of the literature revealed various techniques for determining STG in pharmacological formulations or biological fluids, that may include spectrophotometry [14][15][16], spectrofluorometry [16], thin layer chromatography (TLC), high-performance liquid chromatography (HPLC) [17][18][19][20] and capillary electrophoresis [21]. Since the majority of current techniques have large detection limits and extended study periods [18,20], the majority of these procedures rely on HPLC analyses, which are costly, time-consuming, and need significant quantities of toxic organic solvents.…”