A responsive spectrofluorimetric method was developed for the determination of sitagliptin phosphate using L‐tyrosine as a fluorescence probe. The fluorescence intensity of L‐tyrosine was quenched with sitagliptin phosphate. The fluorescence intensity was recorded at 307 nm using a 272 nm excitation wavelength. The calibration plot between fluorescence intensity and the concentration of drug was liner in the range of 0.1 to 2.0 mM with a good correlation value of 0.997. The limit of detection and quantification were established to be 3.7 × 10‐4 and 1.23 × 10‐3 mM, respectively. Commonly used excipients did not interfere with sitagliptin phosphate measurement. The proposed method was used to measure the sitagliptin phosphate in its standard type, dosage form, and biological samples. The percent recovery were ranged from 97.41–103.36 %. The static quenching was shown to be responsible for quenching as indicated by the Stern–Volmer plot. The method was validated usin ICH guidelines and profitably applied for the content uniformity test, ensuing in a high percent recovery and small relative standard deviation. The proposed approach is effortless, susceptible, selective, economic, and provides a high precision and accuracy, and can be used to determine sitagliptin phosphate in pharmaceutical industry.

The synthesis and spectroscopic characterization of the glutaric acid-amide derivative, 2,4-Cl2C6H3N(H)C(=O)(CH2)3C(=O)OH (1), are described. The X-ray crystal structure determination of (1) shows the backbone of the molecule to be kinked about the methylene-C–N(amide) bond as seen in the C(p)–N–C(m)–C(m) torsion angle of −157.0(2)°; m = methylene and p = phenyl. An additional twist in the molecule is noted between the amide and phenyl groups as reflected in the C(m)–N–C(p)–C(p) torsion angle of 138.2(2)°. The most prominent feature of the molecular packing is the formation of supramolecular tapes assembled through carboxylic acid-O–H…O(carbonyl) and amide-N–H…O(amide) hydrogen bonding.

Chlorpyrifos belongs to an organophosphate pesticides group that is frequently used to increase the yield of crops by controlling plant and pests-related diseases. Chlorpyrifos contaminates the environment and cause diseases to the human population. Molecularly imprinting technology lead to the development of molecularly imprinted polymers having templated oriented cavities with high selectivity, sensitivity, stability, and portability. Our review article aims to provide a collective study related to pesticides detection through molecularly imprinted polymers with existed constraints and necessary potential facets are discussed.

Background: This is fast age where many underlying health issues go unaddressed in race of progress such as Burnout. This state of mental illness due to chronic stress that may be comprised of emotional exhaustion, personal accomplishment and depersonalization. This is thought to be associated with occupation and organizational parameters. This can put physiotherapist compromised health, social and family life, dealing with patients and low performance at work. Objective: To determine burnout level and its severity among physical therapists and associated organizational factors Material and method: Cross sectional survey were conducted in sample of convenience comprising 120 physiotherapists. The participants were of both gender and age above 25 years. The data was collected by using Maslach Burnout Inventory Scale and data analysis was executed using SPSS version 20. Continuous variables including age, total scores were analyzed for mean and standard deviation, while frequency percentages were calculated against categorical variables. Results: Results of the study demonstrated that mean +SD score for emotional exhaustion was 16.55+ 5.07, mean +SD score for personal accomplishment was 44.73+1.54 and mean +SD score for depersonalization was 0.75+0.93. Conclusion: The study concluded that physiotherapists demonstrated mild to moderate level of burnout. Burnout symptoms apparently found significantly associated with high working hours, private sector, female gender and less physical activity.

OBJECTIVE: To determine the frequency of hyperuricemia in type 2 diabetes mellitus (T2DM) patients with BMI >23 kg/m2 MATERIALS AND METHODS: This descriptive study was conducted from February 2020 to September 2021. All patients with Type 2 Diabetes Mellitus (T2DM )with body mass index (BMI) >23 kg/m2 were assessed for hyperuricemia through Roche Cobas 6000 series C501 in MTI Hayatabad Medical Complex’s laboratory.      RESULTS: Total number of patients was 300, out of which 60 % were males. The studied population has a mean age of 59 (SD =±7) years, mean systolic blood pressure was 151 (SD = ±17) mmHg, mean duration of T2DM was 13 (SD = ±4) years, mean HbA1c was 10.9 (SD = ±2.5) %, mean BMI was 28.8 (SD = ±3.1) kg/m2 and mean serum uric acid was 5.7 (SD=±1.3) mg/dl. The overall prevalence of Hyperuricemia was 47% (36.7 % males and 62.5% females). Of those with hyperuricemia, 73 % were also found to have hypertension. Results showed that patients with hyperuricemia belonged to older age, and have higher Systolic blood pressure, raised BMI, and HbA1c. The mean differences were considered statistically significant with a p-value < 0.05 by using an independent sample t-test. CONCLUSION: The current study demonstrated a higher prevalence of hyperuricemia in T2DM patients with BMI> 23 kg/m2. Patients with hyperuricemia had a higher mean HbA1c, higher mean BMI, and raised systolic component of blood pressure. KEY WORDS: BMI, Hyperuricemia, Hypertension, Type 2 diabetes mellitus, HbA1c