Proteotyping: Proteomic characterization, classification and identification of microorganisms – A prospectus

Karlsson, Roger; Gonzales-Silès, Lucia; Boulund, Fredrik; Svensson-Stadler, Liselott; Skovbjerg, Susann; Karlsson, Anders; Davidson, Max; Hulth, Stefan; Kristiansson, Erik; Moore, Edward R. B.

doi:10.1016/j.syapm.2015.03.006

Cited by 88 publications

(71 citation statements)

References 83 publications

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“…; Karlsson et al . ). Therefore, in order to achieve reliable and reproducible species‐level identification of micro‐organisms from MALDI‐TOF MS identification, standardized culture procedures have been adopted.…”

Section: Methodsmentioning

confidence: 97%

“…This approach has been reported to be able to yield effective species‐level identification (Karlsson et al . ; Patel ). Particularly, this method has been shown to be suitable for a rapid identification of different species belonging to the genus Mycobacteria , but some complications for the differentiation between M. tuberculosis and M. bovis were reported (Shitikov et al .…”

Section: Introductionmentioning

confidence: 98%

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Exploring MALDI-TOF MS approach for a rapid identification ofMycobacterium aviumssp.paratuberculosisfield isolates

et al. 2017

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The gold standard test for the diagnosis of paratuberculosis is still isolation of MAP by cultural methods, but additional assays, such as qPCR and subculturing for determination of mycobactin dependency are required to confirm its identification. We have provided here evidence pertaining to the usefulness of MALDI-TOF MS approach for a rapid identification of this mycobacterium among other members of M. avium complex.

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Section: Methodsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 98%

Exploring MALDI-TOF MS approach for a rapid identification ofMycobacterium aviumssp.paratuberculosisfield isolates

et al. 2017

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“…Next‐generation proteomics allows the detection of numerous peptides by tandem mass spectrometry . These high‐resolution markers can be used to decipher the origin of the proteins present in a sample based on their taxon specificity . Here, a new metaproteomic shotgun strategy was used to rapidly analyze the contents of the vaccines and to estimate the ratio of antigen versus production contaminants.…”

Section: Taxon‐specific Peptide Counts Observed By Shotgun Proteomicsmentioning

confidence: 99%

Improving Quality Control of Contagious Caprine Pleuropneumonia Vaccine with Tandem Mass Spectrometry

et al. 2018

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Vaccines to protect livestock against contagious caprine pleuropneumonia (CCPP) consist of inactivated, adjuvanted antigens. Quality control of these vaccines is challenging as total protein quantification provides no indication of protein identity or purity, and culture is not an option. Here, a tandem mass spectrometry approach is used to identify the mycoplasma antigen contained in reference samples and in commercial CCPP vaccines. By the same approach, the relative amounts of mycoplasma antigen and residual proteins originating from the production medium are determined. Mass spectrometry allows easy and rapid identification of the peptides present in the vaccine samples. Alongside the most probable mycoplasma species effectively present in the vaccines, a very high proportion of peptides from medium constituents are detected in the commercial vaccines tested.

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“…MALDI MS ions are singly charged, representing the non-fragmented parent ion mass, the resulting spectra is simple, and therefore, the species of unknown samples can be easily deduced after data processing and direct pattern matching with that of the spectra established from well-defined species compiled as a database. The identity of spectral peaks or protein sequence information is not important, as the species deduced by matching the protein profiles usually at a range of 2–20 kDa (2,000–20,000 m/z) (Evason et al, 2001; Sauer and Kliem, 2010; Welker and Moore, 2011; Karlsson et al, 2015; Cassagne et al, 2016). …”

Section: Maldi Ms Basis Of Species Identificationmentioning

confidence: 99%

MALDI-TOF MS Profiling-Advances in Species Identification of Pests, Parasites, and Vectors

Murugaiyan

Roesler

2017

Front. Cell. Infect. Microbiol.

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Invertebrate pests and parasites of humans, animals, and plants continue to cause serious diseases and remain as a high treat to agricultural productivity and storage. The rapid and accurate species identification of the pests and parasites are needed for understanding epidemiology, monitoring outbreaks, and designing control measures. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling has emerged as a rapid, cost effective, and high throughput technique of microbial species identification in modern diagnostic laboratories. The development of soft ionization techniques and the release of commercial pattern matching software platforms has resulted in the exponential growth of applications in higher organisms including parasitology. The present review discusses the proof-of-principle experiments and various methods of MALDI MS profiling in rapid species identification of both laboratory and field isolates of pests, parasites and vectors.

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Proteotyping: Proteomic characterization, classification and identification of microorganisms – A prospectus

Cited by 88 publications

References 83 publications

Exploring MALDI-TOF MS approach for a rapid identification ofMycobacterium aviumssp.paratuberculosisfield isolates

Exploring MALDI-TOF MS approach for a rapid identification ofMycobacterium aviumssp.paratuberculosisfield isolates

Improving Quality Control of Contagious Caprine Pleuropneumonia Vaccine with Tandem Mass Spectrometry

MALDI-TOF MS Profiling-Advances in Species Identification of Pests, Parasites, and Vectors

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