Protist community composition during spring in an Arctic flaw lead polynya

Terrado, Ramón; Medrinal, Emmanuelle; Dasilva, Cindy; Thaler, Mary; Vincent, Warwick F.; Lovejoy, Connie

doi:10.1007/s00300-011-1039-5

Cited by 61 publications

(80 citation statements)

References 76 publications

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“…Laurent (2007), andSir Wilfred Laurier (2008). Details of these missions have been reported elsewhere (Barber et al, 2010(Barber et al, , 2012Comeau et al, 2011;Terrado et al, 2011).…”

Section: Sample Collection Extraction and Sequencingmentioning

confidence: 99%

“…All PC filters were placed in 2-mL cryovials. Prior to 2010, DNA was preserved by adding 1.8 mL of lysis buffer to the Sterivex units (0.2-3 µm fraction) and cryovials (3-50 µm fraction), and RNA was preserved in RLT buffer (Qiagen) as in Terrado et al (2011) and Thaler and Lovejoy (2015). Samples were either frozen immediately at −80 • C or placed in liquid nitrogen after adding buffer.…”

Section: Sample Collection Extraction and Sequencingmentioning

confidence: 99%

“…comm.). For samples collected before 2010, DNA was extracted from the filters as in Terrado et al (2011) using a salt based method (Aljanabi and Martinez, 1997) while RNA was extracted using the RNAEasy micro Kit (Qiagen). The sample collected in 2010 was extracted using the AllPrep DNA/RNA Mini Kit (Qiagen) with DNA and RNA from the same filters.…”

Section: Sample Collection Extraction and Sequencingmentioning

confidence: 99%

“…All OTU representative reads were aligned in PyNast (Caporaso et al, 2010a), manually curated in BioEdit v.7.2.5 (Hall, 1999) and used to construct a phylogenetic tree (FastTree; Price et al, 2010). Assignment of taxonomic identity was performed in mothur with a 0.8 confidence threshold against the Northern Reference Database v.1.0 , which follows the Silva taxonomy but includes high-quality longer environmental sequences from the Arctic and North Atlantic (Terrado et al, 2009(Terrado et al, , 2011Charvet et al, 2012;Monier et al, 2013;Dasilva et al, 2014). However, using this database, around 30-45% of the reads were still not classified beyond "Other Dinoflagellates" or "Other Ciliates.…”

Section: Post-sequence Data Processing and Taxonomic Classificationmentioning

confidence: 99%

“…Dinoflagellates and ciliates persist over winter darkness in the Arctic (Terrado et al, 2011;Marquardt et al, 2016), but their seasonal progression is poorly understood. To resolve temporal patterns at finer taxonomic resolution, and to test for trends consistent with environmental selection among Arctic microzooplankton, we investigated samples collected from November 2007 to July 2008 during the International Polar Year Circumpolar Flaw Lead Study (IPY-CFL; Barber et al, 2010Barber et al, , 2012.…”

Section: Introductionmentioning

confidence: 99%

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Seasonal and Interannual Changes in Ciliate and Dinoflagellate Species Assemblages in the Arctic Ocean (Amundsen Gulf, Beaufort Sea, Canada)

et al. 2017

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Recent studies have focused on how climate change could drive changes in phytoplankton communities in the Arctic. In contrast, ciliates and dinoflagellates that can contribute substantially to the mortality of phytoplankton have received less attention. Some dinoflagellate and ciliate species can also contribute to net photosynthesis, which suggests that species composition could reflect food web complexity. To identify potential seasonal and annual species occurrence patterns and to link species with environmental conditions, we first examined the seasonal pattern of microzooplankton and then performed an in-depth analysis of interannual species variability. We used high-throughput amplicon sequencing to identify ciliates and dinoflagellates to the lowest taxonomic level using a curated Arctic 18S rRNA gene database. DNA-and RNA-derived reads were generated from samples collected from the Canadian Arctic from November 2007 to July 2008. The proportion of ciliate reads increased in the surface toward summer, when salinity was lower and smaller phytoplankton prey were abundant, while chloroplastidic dinoflagellate species increased at the subsurface chlorophyll maxima (SCM), where inorganic nutrient concentrations were higher. Comparing communities collected in summer and fall from 2003 to 2010, we found that microzooplankton community composition change was associated with the record ice minimum in the summer of 2007. Specifically, reads from smaller predatory species like Laboea, Monodinium, and Strombidium and several unclassified ciliates increased in the summer after 2007, while the other usually summer-dominant dinoflagellate taxa decreased. The ability to exploit smaller prey, which are predicted to dominate the future Arctic, could be an advantage for these smaller ciliates in the wake of the changing climate.

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“…Laurent (2007), andSir Wilfred Laurier (2008). Details of these missions have been reported elsewhere (Barber et al, 2010(Barber et al, , 2012Comeau et al, 2011;Terrado et al, 2011).…”

Section: Sample Collection Extraction and Sequencingmentioning

confidence: 99%

Section: Sample Collection Extraction and Sequencingmentioning

confidence: 99%

Section: Sample Collection Extraction and Sequencingmentioning

confidence: 99%

Section: Post-sequence Data Processing and Taxonomic Classificationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Seasonal and Interannual Changes in Ciliate and Dinoflagellate Species Assemblages in the Arctic Ocean (Amundsen Gulf, Beaufort Sea, Canada)

et al. 2017

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Sea ice as a habitat for micrograzers

Caron

Gast

Garneau

2016

Sea Ice

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Rubisco in high Arctic tidewater glacier‐marine systems: A new window into phytoplankton dynamics

Roberts,

Bhatia,

Rowland

et al. 2024

Limnology & Oceanography

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The hundreds of tidewater glaciers found in the Canadian Arctic Archipelago have the potential to enhance delivery of nutrients and other material to the surface ocean. Despite this, their influence on marine ecosystems, specifically phytoplankton, is poorly characterized. Here we developed and applied a quantitative mass spectrometry‐based approach to measure phytoplankton ribulose‐1,5‐bisphosphate carboxylase/oxygenase (Rubisco) concentrations to examine differences in productivity in glacierized and non‐glacierized marine systems in Jones Sound, Nunavut, within Inuit Nunangat. Comparisons to chloroplast 16S rRNA gene amplicon sequencing data suggested that these measurements detect the majority of Rubisco produced in Jones Sound. Because Rubisco catalyzes carbon fixation, we used these measurements to estimate total and group‐specific primary production potential, which were within the range of historical primary production measurements made using classical methods in this region. Our measurements also revealed that up to 2% of total protein in the water column is Rubisco, and that Rubisco concentrations are correlated with chlorophyll fluorescence, with maxima near the nitracline. Rubisco produced by diatom genera Chaetoceros and Thalassiosira were higher in marine regions influenced by glaciers, while Rubisco from Micromonas (Chlorophyta) was greater in non‐glacierized regions. This suggests that future climate scenarios may favor smaller phytoplankton groups, like Micromonas, with consequences for food webs and carbon cycling. This study broadens our understanding of how tidewater glaciers will impact phytoplankton communities, now and in a warmer future, and lays the foundation for using this mass spectrometry‐based approach to quantify phytoplankton group‐specific carbon fixation potential in other marine regions.

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Protist community composition during spring in an Arctic flaw lead polynya

Cited by 61 publications

References 76 publications

Seasonal and Interannual Changes in Ciliate and Dinoflagellate Species Assemblages in the Arctic Ocean (Amundsen Gulf, Beaufort Sea, Canada)

Seasonal and Interannual Changes in Ciliate and Dinoflagellate Species Assemblages in the Arctic Ocean (Amundsen Gulf, Beaufort Sea, Canada)

Sea ice as a habitat for micrograzers

Rubisco in high Arctic tidewater glacier‐marine systems: A new window into phytoplankton dynamics

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