Protocol for a Binary Choice Feeding Assay Using Adult, Axenic Drosophila

Charroux, Bernard; Royet, Julien

doi:10.1016/j.xpro.2020.100117

Cited by 4 publications

(3 citation statements)

References 8 publications

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“…S6A; left panel). This design was adapted from a previous study (Charroux and Royet 2020). 0 - 4 day old male flies were sorted under CO 2 anesthesia in groups of 15 - 20 and habituated in DIETS vial with CD for 2 days.…”

Section: Methodsmentioning

confidence: 99%

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DIETS: a simple and sensitive assay to measure and control the intake of complex solid foods, like high-fat diets, inDrosophila

Thakare,

Choudhary,

Pydah

et al. 2023

Preprint

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InDrosophila, an organism much used for dietary studies, comprehending the regulation of feeding and the effect of diet requires accurate measurement of food intake. Although flies are normally housed on agar-based foods, direct estimation of solid feeding has been thought infeasible due to the minute amounts they consume. Moreover, it has been technically challenging to precisely restrict the amount of food flies consume during an experiment. Here, we report a label-free, sensitive, and precise assay, DIETS (Direct Intake Estimation and Tracking of Solid food consumption), that is compatible with videography. In DIETS, food consumed by a group of flies, from a small cup is directly weighed over hours to days. Dietary choice is measured in the assay by weighing food intake from two cups with distinct diets. DIETS enables the quantification of a high-fat diet intake over days. Notably, by limiting food in the cups, we could precisely implement targeted dietary restrictions in flies without diluting nutrients or causing compensatory feeding.

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Section: Methodsmentioning

confidence: 99%

“…These images were later compressed into videos of 10 fps using ImageJ ( version 1.53t). A previously published MATLAB code (Charroux and Royet 2020) was modified to quantify the number of flies in a defined area.…”

Section: Methodsmentioning

confidence: 99%

DIETS: a simple and sensitive assay to measure and control the intake of complex solid foods, like high-fat diets, inDrosophila

Thakare,

Choudhary,

Pydah

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…For analysis of feeding behavior, different approaches have been employed for qualitative and quantitative analyses of food consumption. These include binary-choice feeding assays ( Charroux and Royet, 2020 ; Tanimura et al., 1982 ; Zhang et al., 2013 ), the capillary feeder (CAFE) assay ( Ja et al., 2007 ), Fly Liquid-Food Interaction Counter (FLIC) assay ( Ro et al., 2014 ), radiolabel-based feeding ( Deshpande et al., 2014 ; Thompson and Reeder, 1987 ), electronic-based feeding assays ( Itskov et al., 2014 ; Ro et al., 2014 ), and Droso-X assay ( Aryal and Lee, 2021 ; Sang et al., 2021 ). Binary-choice feeding assays are the most widely used behavioral approaches to monitor feeding, since they are simple to perform, safe, relatively inexpensive, can be used in a classroom setting and can be applied to large-scale genetic screening.…”

Section: Before You Beginmentioning

confidence: 99%

Protocol for binary food choice assays using Drosophila melanogaster

et al. 2022

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DIETS: a simple and sensitive assay to measure and control the intake of complex solid foods, like high-fat diets, in Drosophila

Thakare,

Choudhary,

Pydah

et al. 2024

Preprint

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The fruit fly Drosophila melanogaste r offers a powerful model to study how diet affects the body and brain. However, existing methods for measuring their food intake often rely on dyes or tags mixed with food, which can be inaccurate due to how the flies absorb and eliminate them. Capillary-based assays like CAFE directly measure consumption, but only work with liquids and shorten fly lifespan. Additionally, capillary assays are incompatible with delivering viscous foods like high-fat diets. Even solidified high-fat diets tend to be sticky death traps for flies. Another longstanding challenge for fly researchers is that dietary restriction in flies involves diluting food, leading to compensatory feeding. To address these shortcomings, we have developed DIETS, a sensitive feeding assay that can be implemented even in low-resource settings. DIETS eliminates the need for labels and directly weighs the solid food consumed by small groups of flies over extended periods of hours to weeks. It allows us to deliver precise amounts of food to flies and implement accurate dietary restrictions. Importantly, DIETS is compatible with studying energy-dense high-fat diets. Using DIETS, we observed that, unlike a high-sugar diet, an isocaloric high-fat diet did not improve the flies’ ability to withstand starvation, even though they consumed more calories and had higher fat deposition.

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Protocol for a Binary Choice Feeding Assay Using Adult, Axenic Drosophila

Cited by 4 publications

References 8 publications

DIETS: a simple and sensitive assay to measure and control the intake of complex solid foods, like high-fat diets, inDrosophila

DIETS: a simple and sensitive assay to measure and control the intake of complex solid foods, like high-fat diets, inDrosophila

Protocol for binary food choice assays using Drosophila melanogaster

DIETS: a simple and sensitive assay to measure and control the intake of complex solid foods, like high-fat diets, in Drosophila

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