Protocol for wheat (Triticum aestivum L.) anther culture

Pauk, János; Mihály, Róbert; Puolimatka, Matti

doi:10.1007/978-94-017-1293-4_10

Cited by 42 publications

(44 citation statements)

References 4 publications

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“…Based on ploidy analysis, a similar value (34.4%) was measured for this phenomenon in AC of 'GK Fehér' spelt wheat. This value can be increased by the application of an effective chromosome doubling method (Barnabás et al, 1991, Pauk et al, 2003.…”

Section: Discussionmentioning

confidence: 99%

“…After a four-week cultivation period, the first ELS the size of 1-2 mm were transferred onto 90 mm diameter plastic Petri dishes (Sarstedt, Newton, MA, USA) containing 190-2Cu solidified regeneration medium (Pauk et al, 2003). The transfer of ELS of the optimal size was repeated once a week.…”

Section: Plant Regeneration and Ploidy Level Determinationmentioning

confidence: 99%

“…After acclimatization, the transplanted plantlets were grown following the standard cereal growing greenhouse protocol. The ploidy levels of the acclimatized plants were determined using the measurement of stomata length and flow cytometry detailed in earlier publications (Pauk et al, 2003;Lantos et al, 2012). Furthermore, the seed set production of AC-derived plants was recorded after the harvest.…”

Section: Plant Regeneration and Ploidy Level Determinationmentioning

confidence: 99%

See 2 more Smart Citations

High Frequency of Doubled Haploid Plant Production in Spelt Wheat

Lantos

Jenes

Bóna

et al. 2016

Acta Biologica Cracoviensia S. Botanica

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This is the first study to report an efficient anther culture (AC) method for spelt wheat, which has an increasing importance not only in applied research but also in organic farming and changing nutritional standards. In this study, an efficient AC protocol has been described for 'GK Fehér' spelt wheat. The number of AC-derived embryolike structures (ELS) was 62.2/100 anthers, from which we were able to regenerate 30.6 green plantlets per 100 anthers. The percentage of green plantlets production was 89.0% among the regenerated plantlets, while the phenomenon of albinism was restricted (3.8/100 anthers). Altogether, from AC of 'GK Fehér' 306 green plantlets were produced in vitro and 241 plants were acclimatized to the greenhouse conditions. Based on ploidy level analyses, 83 spontaneous doubled haploid (DH) plants were produced (8.3 DH plants/100 anthers), so the percentage of spontaneous rediploidization was 34.4%. The spontaneous DH plants produced fertile spikes, while a few seeds were harvested from seven partially fertile plants.

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Section: Discussionmentioning

confidence: 99%

Section: Plant Regeneration and Ploidy Level Determinationmentioning

confidence: 99%

Section: Plant Regeneration and Ploidy Level Determinationmentioning

confidence: 99%

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High Frequency of Doubled Haploid Plant Production in Spelt Wheat

Lantos

Jenes

Bóna

et al. 2016

Acta Biologica Cracoviensia S. Botanica

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“…Plant regeneration: After 4 weeks of culture, the first microsporederived ELS became visible in the liquid medium. Structures that were 2 mm in size were transferred each week into 90-mm diameter plastic Petri dishes (Sarstedt, Newton, MA, USA) containing 190-2Cu regeneration medium (Pauk et al 2003). Approximately 40-50 ELS were placed into each Petri dish containing regeneration medium.…”

Section: Methodsmentioning

confidence: 99%

Efficient application of in vitro anther culture for different European winter wheat (Triticum aestivum L.) breeding programmes

et al. 2013

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The efficiency of our anther culture protocol was tested with high‐ and low‐responding genotypes, ‘Svilena’ and ‘Berengar’, and 93 F1 winter wheat crosses in 2010 and 2011. Based on data for these genotypes, the effect of genotype influenced the number of embryo‐like structures, regenerated plantlets and green plantlets, while the number of albino plantlets was affected by genotype, year and environmental factors. Although genotype also influenced the production of green plantlets from breeding crosses, with green plantlets per 100 anthers ranging from 0.04 to 28.67, the average regeneration rate over all crosses was 5.3 green plantlets/100 anthers, which resulted in a total of 11 416 well‐rooted green plantlets. The survival rate of green plantlets following acclimatization was 97.21% in 2010 and 96.34% in 2011. In this study, the phenomenon of albinism and genotype dependency did not hinder the production of more than five thousand green plantlets each year. In our experiments, anther culture proved to be an efficient method in winter wheat breeding programmes with lower costs than alternative technologies.

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“…Dihaploid hatların İ kullanılmasıyla genetik ve ıslah çalışmalarını yapmak hızlanmakta ve kolaylaşmaktadır. Anter kültürü, yaygın olarak desteklenen, buğdaydaki gelişmeler için homozigot double haploid hatlar üretmekte kullanılır ve bu son birkaç yıl içinde birçok ıslah programı arasında gitgide önemli bir teknik haline gelmiştir (Henry and De Buyser 1990 (Pauk et al 2003). Bu yüzden araştırmamızda ıslah programında yer alan F 2 buğday popülasyonundan faydalanılmıştır.…”

Section: Introductionunclassified

Anter Kültürü Tekniği ile Ekmeklik Buğday (Triticum aestivum L.) Melez Populasyonlarından Doubled Haploid Bitkilerin Elde Edilmesi

Başer¹,

Korkut²,

Oğuz³

et al. 2016

Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi

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ÖzÇalışmada, Namık Kemal Üniversitesi Ziraat Fakültesi Tarla Bitkileri Bölümünde elde edilen 17 F2 ekmeklik buğday melez popülasyonu materyal olarak kullanılmıştır. Ekmeklik buğday genotipleride erkenorta tek çekirdekli dönemde çiçek tozu içeren anterler W14 besi ortamında kültüre alınmışlardır. Bu ortamda gelişim gösteren kalluslar ve yeşil bitkicikler 190Icu rejenerasyon ortamına aktarılarak gelişimleri sağlanmıştır. Çalışmada anterlerden gelişen kallus sayısı, albino bitkicik sayısı, test tüplerine aktarılan yeşil bitkicik sayısı, toprağa aktarılan yeşil bitki sayısı ve spontan double haloid bitki sayıları belirlenmiştir. Yapılan önemlilik testi sonucunda melez popülasyonlarında incelenen tüm karakterlerde genotipler arasında istatistiki olarak önemli farklılık bulunmuştur. Yeşil bitkicik sayısı 1.0-25.25, kallus sayısı 128.75-2.25, tüplere aktarılan bitkicik sayısı 1.0-24.0 adet, toprağa aktarılan bitki sayısı ortalama olarak 0.00-17.50 adet, spontan double haploid bitki oranı %0.00-55.00 arasında değişmiştir. En fazla seraya aktarılan bitki sayısı 17.30 adet ile Sagittario x Pehlivan ve 9.50 adet ile Sagittario x Luzanovka melez popülasyonundan elde edilmiştir.

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Protocol for wheat (Triticum aestivum L.) anther culture

Cited by 42 publications

References 4 publications

High Frequency of Doubled Haploid Plant Production in Spelt Wheat

High Frequency of Doubled Haploid Plant Production in Spelt Wheat

Efficient application of in vitro anther culture for different European winter wheat (Triticum aestivum L.) breeding programmes

Anter Kültürü Tekniği ile Ekmeklik Buğday (Triticum aestivum L.) Melez Populasyonlarından Doubled Haploid Bitkilerin Elde Edilmesi

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