Protocols for preparation of platelet rich plasma (PRP) in Quarter Horses

Miranda, Stephania; Costa, Maria Fernanda Mello; Rebouças, Natália; Ramos, Márcia T.; Lessa, Daniel Augusto Barroso; Alencar, Nayro Xavier de

doi:10.1590/1678-5150-pvb-5883

Cited by 4 publications

(6 citation statements)

References 51 publications

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“…highlight that only one paper presented a complete description of the platelets, leukocytes and red blood cells for PRP using two different semiautomated devices (69). Several manual methods have been proposed for PRP elaboration from equine blood (19,66,68,70,77). In general, these protocols include one or two centrifugation steps (73).…”

Section: Equine Prp-related Hemocomponentsmentioning

confidence: 99%

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A Critical Overview of the Use of Platelet-Rich Plasma in Equine Medicine Over the Last Decade

2021

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In the 1990s, the role of platelets in inflammation and tissue healing was finally recognized. Since then, the clinical use of platelet-derived products (hemocomponents), such as, platelet-rich plasma (PRP), markedly increased. The promise of a more economical option of a disease-modifying treatment led to the intensive and continuous research of PRP products and to its widespread clinical use. A number of protocols and commercial kits have been developed with the intention of creating a more practical and reliable option for clinical use in equine patients. Still, the direct comparison between studies is particularly challenging due to the lack of standardization on the preparation methods and product composition. The incomplete reports on PRP cellular concentration and the poorly designed in vivo studies are additional matters that contest the clinical efficiency of this biomaterial. To overcome such challenges, several in vitro and in vivo studies have been proposed. Specifically, experiments have greatly focused in protocol optimization and its effect in different tissues. Additionally, in vivo studies have proposed different biological products envisioning the upgrade of the anti-inflammatory cytokines trusting to increase its anti-inflammatory effect. The individual variability and health status of the animal, type of tissue and condition treated, and protocol implemented are known to influence on the product's cell and cytokine composition. Such variability is a main clinical concern once it can potentially influence on PRP's therapeutic effects. Thus, lack of qualitative and quantitative evidence-based data supporting PRP's clinical use persists, despite of the numerous studies intended to accomplish this purpose. This narrative review aims to critically evaluate the main research published in the past decade and how it can potentially impact the clinical use of PRP.

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Section: Equine Prp-related Hemocomponentsmentioning

confidence: 99%

“…Several manual methods have been proposed for PRP elaboration from equine blood ( 19 , 66 , 68 , 70 , 77 ). In general, these protocols include one or two centrifugation steps ( 73 ).…”

Section: Equine Prp-related Hemocomponentsmentioning

confidence: 99%

A Critical Overview of the Use of Platelet-Rich Plasma in Equine Medicine Over the Last Decade

2021

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“…Sixty-nine hours after R1 all horses had blood samples collected for laboratory analysis and PRP preparation: 5mL of venous blood was collected into a vacuum tube containing EDTA 7.2mg (Vacutainer™, Becton Dickinson), and 180mL of venous blood was collected into 3.2% sodium citrate vacuum tubes (Vacutainer™, Becton Dickinson). The samples in EDTA were used for complete blood count, protein, and fibrinogen measurements, while the samples in sodium citrate were used for PRP preparation following the methodology described by (Miranda et al, 2019).…”

Section: Effect Of Intrabronchial…mentioning

confidence: 99%

Effect of intrabronchial platelet rich plasma on the exercise-induced pulmonary hemorrhage endoscopic score in thoroughbred racehorses using furosemide: a preliminary study

Pires

Miranda

Costa

et al. 2021

Arq. Bras. Med. Vet. Zootec.

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The high prevalence of exercise-induced pulmonary hemorrhage (EIPH) in athletic horses constitutes to be a challenge to the racing industry and a source of major concern to animal welfare. Both experimental and clinical evidence indicate that the use of autologous platelet-rich plasma (PRP) is a promising effector of repair in a variety of pulmonary conditions. The present study evaluated the effect of intrabronchial instillation of PRP on EIPH endoscopic scores from 37 Thoroughbred racehorses. Inclusion criteria were for animals to be EIPH-positive in, at least, two consecutive post-exercise endoscopic exams and to receive 250mg of furosemide IV four hours before racing. Animals were randomly assigned into 3 groups: placebo, control, and PRP instillation. All 37 Thoroughbred racehorses included had EIPH endoscopic scores pre- and post- treatment compared by statistical analysis. The bleeding score from the group receiving PRP was significantly lower than in the control and placebo groups. No adverse effects were observed in any animal during or after the experiment. It was possible to conclude that the intrabronchial instillation of autologous PRP was effective in reducing EIPH scores in racehorses receiving furosemide and that this bioproduct can be considered as a promising coadjuvant in controlling EIPH in athletic horses.

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“…Various protocols have been developed for obtaining PRP, but these protocols vary widely in amount of blood collected volume, number of centrifugations and speed, and equipment used (Franklin et al, 2015;Conceição et al, 2017;Miranda et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Pre or perioperative period: when is the best time to produce platelet-rich plasma in dogs?

et al. 2022

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When evaluating published studies on the production of platelet-rich plasma (PRP), it is possible to observe that there is no consensus on when blood should be collected and processed. Some perform blood collection before surgery, prior to the administration of drugs, fluids, or any surgical trauma, while others collect blood during the perioperative period. The present objective was to determine the best moment for PRP production based on the platelet count. Blood samples were collected from 20 healthy female dogs at two distinct moments: before surgery, prior to anesthesia and fluid therapy (M0), and later, in the operation room, after administration of the anesthetics and intravenous fluids (M1). To obtain PRP, blood was centrifuged at 1200 rpm for ten minutes, then at 1600 rpm for ten minutes. Results showed that the blood collected at M1 had a 29% decrease in platelet count compared to M0 (highly significant statistical difference). It is recommended that blood collection, which is an essential step for producing platelet-rich plasma, shall be done during the pre-operative period.

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Protocols for preparation of platelet rich plasma (PRP) in Quarter Horses

Cited by 4 publications

References 51 publications

A Critical Overview of the Use of Platelet-Rich Plasma in Equine Medicine Over the Last Decade

A Critical Overview of the Use of Platelet-Rich Plasma in Equine Medicine Over the Last Decade

Effect of intrabronchial platelet rich plasma on the exercise-induced pulmonary hemorrhage endoscopic score in thoroughbred racehorses using furosemide: a preliminary study

Pre or perioperative period: when is the best time to produce platelet-rich plasma in dogs?

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