Protocols for the Detection and Chemical Characterisation of Microbial Glycolipids

Smyth, Thomas J.; Rudden, Michelle; Tsaousi, Konstantina; Marchant, Roger; Banat, İbrahim M.

doi:10.1007/8623_2014_25

Cited by 20 publications

(22 citation statements)

References 55 publications

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“…Rhamnolipid extraction was carried out using an acid precipitation and solvent extraction method (Smyth et al 2014). First, cultures were centrifuged at 13,000× g for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Fatty acid synthesis pathway provides lipid precursors for rhamnolipid biosynthesis in Burkholderia thailandensis E264

Irorere

Smyth

Cobice

et al. 2018

Appl Microbiol Biotechnol

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Rhamnolipid production was monitored for a period of 216 h using different substrates in Pseudomonas aeruginosa PAO1 and Burkholderia thailandensis E264 which showed comparable crude yields attained by both after 216 h. The crude yield for P. aeruginosa, however, was significantly higher at the early stages of fermentation (72 or 144 h). Additionally, P. aeruginosa produced rhamnolipid with odd and even carbon chain lipid moieties using odd carbon chain fatty acid substrates (up to 45.97 and 67.57%, respectively). In contrast, B. thailandensis produced rhamnolipid with predominantly even carbon chain lipid moieties (up to 99.26). These results indicate the use of the fatty acid synthesis (FAS II) pathway as the main source of lipid precursors in rhamnolipid biosynthesis by B. thailandensis. Isotope tracing using 0.25% stearic acid – d35 + 1% glycerol as carbon substrate showed a single pattern of deuterium incorporation: with predominantly less than 15 deuterium atoms incorporated into a single Di-C14-C14 rhamnolipid molecule. This further indicates that the FAS II pathway is the main source of the lipid precursor in rhamnolipid biosynthesis by B. thailandensis. The pathogenicity of these strains was also assessed, and results showed that B. thailandensis is significantly less pathogenic than P. aeruginosa with an LC50 at 24 h > 2500, approximately three logs higher than P. aeruginosa using the Galleria mellonella larva model.Electronic supplementary materialThe online version of this article (10.1007/s00253-018-9059-5) contains supplementary material, which is available to authorized users.

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“…Rhamnolipid extraction was carried out using an acid precipitation and solvent extraction method (Smyth et al 2014). First, cultures were centrifuged at 13,000× g for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Fatty acid synthesis pathway provides lipid precursors for rhamnolipid biosynthesis in Burkholderia thailandensis E264

Irorere

Smyth

Cobice

et al. 2018

Appl Microbiol Biotechnol

Self Cite

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“…Before extraction and purification, colorimetric methods can be used to determine the presence of glycolipid biosurfactants in either the culture medium or the extract. These assays are used to detect the sugar moieties of the glycolipids, with the anthrone or orcinol assay being the most commonly used . Thin‐layer chromatography is a simple method that allows the identification of the presence of glycolipids.…”

Section: Dowstream Processing: Extraction and Purification Of Glycolimentioning

confidence: 99%

“…It can also provide preliminary information on the structural types of glycolipids present in crude extracts, at the same time as revealing the presence of purities in crude extracts. Thin‐layer chromatography detection should be carried out before any purification procedures to determine the presence of glycolipids and the purity after the purification steps …”

Section: Dowstream Processing: Extraction and Purification Of Glycolimentioning

confidence: 99%

“…Moreover, HPLC is a relatively common analytical technique that can be used to separate glycolipids. Coupling of HPLC with an evaporative light scattering detector or mass spectrometry provides the valuable information that is needed for the identification and quantification of glycolipids . Table describes the main purification techniques used, along with their advantages, and provides examples from previous studies.…”

Section: Dowstream Processing: Extraction and Purification Of Glycolimentioning

confidence: 99%

“…These assays are used to detect the sugar moieties of the glycolipids, with the anthrone or orcinol assay being the most commonly used. 102 Thin-layer chromatography is a simple method that allows the identification of the presence of glycolipids. It can also provide preliminary information on the structural types of glycolipids present in crude extracts, at the same time as revealing the presence of purities in crude extracts.…”

Section: Dowstream Processing: Extraction and Purification Of Glycolimentioning

confidence: 99%

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Glycolipid biosurfactants: main properties and potential applications in agriculture and food industry

2016

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Glycolipids, consisting of a carbohydrate moiety linked to fatty acids, are microbial surface active compounds produced by various microorganisms. They are characterized by high structural diversity and have the ability to decrease the surface and interfacial tension at the surface and interface, respectively. Rhamnolipids, trehalolipids, mannosylerythritol lipids and cellobiose lipids are among the most popular glycolipids. They have received much practical attention as biopesticides for controlling plant diseases and protecting stored products. As a result of their antifungal activity towards phytopathogenic fungi and larvicidal and mosquitocidal potencies, glycolipid biosurfactants permit the preservation of plants and plant crops from pest invasion. Also, as a result of their emulsifying and antibacterial activities, glycolipids have great potential as food additives and food preservatives. Furthermore, the valorization of food byproducts via the production of glycolipid biosurfactant has received much attention because it permits the bioconversion of byproducts on valuable compounds and decreases the cost of production. Generally, the use of glycolipids in many fields requires their retention from fermentation media. Accordingly, different strategies have been developed to extract and purify glycolipids. © 2016 Society of Chemical Industry

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Analysis of Carbohydrates and Glycoconjugates by Matrix‐assisted Laser Desorption/Ionization Mass Spectrometry: An Update for 2015–2016

Harvey

2021

Mass Spectrometry Reviews

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This review is the ninth update of the original article published in 1999 on the application of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2016. Also included are papers that describe methods appropriate to analysis by MALDI, such as sample preparation techniques, even though the ionization method is not MALDI. Topics covered in the first part of the review include general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation and arrays. The second part of the review is devoted to applications to various structural types such as oligo‐ and poly‐saccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals. Much of this material is presented in tabular form. The third part of the review covers medical and industrial applications of the technique, studies of enzyme reactions and applications to chemical synthesis. The reported work shows increasing use of combined new techniques such as ion mobility and the enormous impact that MALDI imaging is having. MALDI, although invented over 30 years ago is still an ideal technique for carbohydrate analysis and advancements in the technique and range of applications show no sign of deminishing. © 2020 Wiley Periodicals, Inc.

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Protocols for the Detection and Chemical Characterisation of Microbial Glycolipids

Cited by 20 publications

References 55 publications

Fatty acid synthesis pathway provides lipid precursors for rhamnolipid biosynthesis in Burkholderia thailandensis E264

Fatty acid synthesis pathway provides lipid precursors for rhamnolipid biosynthesis in Burkholderia thailandensis E264

Glycolipid biosurfactants: main properties and potential applications in agriculture and food industry

Analysis of Carbohydrates and Glycoconjugates by Matrix‐assisted Laser Desorption/Ionization Mass Spectrometry: An Update for 2015–2016

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