Protoplast fusion in the yeast, Schwanniomyces alluvius

Wilson, J. Jeffrey; Khachatourians, George G.; Ingledew, W. M.

doi:10.1007/bf00422918

Cited by 37 publications

(8 citation statements)

References 11 publications

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“…Although our fusion results fell within the normal range for such events, we did not obtain any stable hybrids even in intraspecific fusion. The situation is obviously different from the cases of Schizosaccharomyces pombe (22) and Schwanniomyces alluvius (23), which were reported to produce stable prototrophic hybrids between intraspecific auxotrophs.…”

Section: Isolation Of Auxotrophscontrasting

confidence: 62%

Ploidy in the asporogenous yeast Candida maltosa, isolation of its auxotrophic mutants and their cell fusion.

Chang

Jung

Suzuki

et al. 1984

J. Gen. Appl. Microbiol.

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Section: Isolation Of Auxotrophscontrasting

confidence: 62%

Ploidy in the asporogenous yeast Candida maltosa, isolation of its auxotrophic mutants and their cell fusion.

Chang

Jung

Suzuki

et al. 1984

J. Gen. Appl. Microbiol.

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“…Media: Media were used as described by Sherman et al, (1974) and Wilson et al, (1982a) except that KCI was replaced by 0.8M D-sorbitol (de van Broock et al, 1980) where required, and the concentration of polyethylene glycol (4000) was 30% (Sipiczki and Ferenczy, 1977a). The enzyme production medium was described by Dhawale and Ingledew (1983).…”

Section: Methodsmentioning

confidence: 99%

“…The spontaneous and induced (by 100 ug/ml p-fluorophenylalanine, Sipiczki and Ferenczy, 1977b) mitotic segregation analyses were performed, and cell-size was determined (Wilson et al, 1982a). Cellvolumes were ccmputed using the equation for an ellipsoid (Sipiczki and Ferenczy, 1977b).…”

Section: Methodsmentioning

confidence: 99%

“…Protoplasts were then washed twice and resuspended in 10ml of PB containing 10raM CaCI. Fusion of PD-511 and JW-4048 was acc~t~lished as described previously with various controls, (Wilson et al, 1982a). Cellular DNA Content: DNA of stationary-phase cells in YPD was extracted and estimated with diphenylamine as described by Stewart (1975) using herring-sperm DNA as standard.…”

Section: Methodsmentioning

confidence: 99%

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Interspecific protoplast Fusion of Schwanniomyces yeasts

Dhawale

Ingledew

1983

Biotechnol Lett

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SUMMARYInterspecific protoplast fusion of multiple auxotrophic mutants of Schwannic~ces castellii and Schwannicmyces alluvius resulted in fusion products displaying good stability and increased ploidy as a consequence of increased DNA content and cell-volume.Following mitotic segregation analysis, recombinants were observed suggesting that protoplast fusion resulted in nuclear fusion.Attempts to isolate NTG -induced derepressed mutants of one fusion product were successful, however such mutants were similar in cell-size and amylolytic activities to the auxotrophic strains from which they were initially derived.

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“…We and others are interested in developing the genetics of members of the budding yeast Schwanniomyces (7,24), particularly in view of their ability to hydrolyze starch (3,5,6,10,15,19,21,23,26,28,29,31,39,40). The 146-kilodalton (kDa) extracellular glucoamylase of Schwanniomyces castellii that we have characterized is of particular interest because it has both a-1,4 and ot-1,6 activity and is thermally labile (29,30).…”

mentioning

confidence: 99%

Expression and regulation of glucoamylase from the yeast Schwanniomyces castellii

Dowhanick¹,

Russell²,

Scherer³

et al. 1990

J Bacteriol

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Expression of the 146-kilodalton (kDa) extracellular glucoamylase by the budding yeast Schwanniomyces castellii is induced by maltose and starch. By use of antiglucoamylase antisera, we found that this expression was regulated at the level of the mRNA, taking place within 30 min after exposure of yeast cells to the respective sugars. Polyacrylamide gel electrophoresis analysis of the in vitro-translated products of total RNA from maltose-treated cells established that the glucoamylase precursor was approximately 120 kDa in size. Stable glucoamylase transcript was not produced in cells exposed to glucose, 2-deoxyglucose, and heat shock. Cells exposed to these two sugars also degraded intracellular and extracellular glucoamylase. In the presence of sugars such as cellobiose, galactose, lactose, and xylose or in the absence of any carbohydrate, a low-level, constitutive-like expression of this preglucoamylase occurred. The nascent glucoamylase underwent at least two posttranslational modifications, resulting in a 138-kDa cell-associated form and the 146-kDa active form that was found free in the medium. These results suggest that glucoamylase expression is tightly regulated similarly to expression of the enzymes responsible for maltose metabolism in Saccharomyces yeasts.

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Protoplast fusion in the yeast, Schwanniomyces alluvius

Cited by 37 publications

References 11 publications

Ploidy in the asporogenous yeast Candida maltosa, isolation of its auxotrophic mutants and their cell fusion.

Ploidy in the asporogenous yeast Candida maltosa, isolation of its auxotrophic mutants and their cell fusion.

Interspecific protoplast Fusion of Schwanniomyces yeasts

Expression and regulation of glucoamylase from the yeast Schwanniomyces castellii

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