Protoplast Isolation and Culture of Woody Plants

Liu, Jihong

doi:10.1007/1-4020-2985-3_43

Cited by 3 publications

(3 citation statements)

References 28 publications

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“…Они показали, что высокий уровень pH делает растения более устойчивыми к повышенному рН уровню среды. Культура протопластов также может вызывать генетическую изменчивость (протоклональную изменчивость) по различным признакам регенерированных растений, например, цитология, морфология и резистентность [22]. Liu и другие (2003) сообщили, что числа хромосом растений, регенерированных из протопластов варьировали от 59 до 310, с разными уровнями плоидности: как анеуплоиды, диплоиды, тетраплоиды или гексаплоиды.…”

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“…Liu и другие (2003) сообщили, что числа хромосом растений, регенерированных из протопластов варьировали от 59 до 310, с разными уровнями плоидности: как анеуплоиды, диплоиды, тетраплоиды или гексаплоиды. В той же самой работе сообщалось об изменении пола, так как треть регенерированных из женских растений A. deliciosa были получены мужскими растениями [22]. Также есть сообщение о морфологических различиях: в длине междоузлий, длине и ширине листа, и длине черешка среди растений-регенерантов, полученных из протопластов A. deliciosa [22].…”

unclassified

“…В той же самой работе сообщалось об изменении пола, так как треть регенерированных из женских растений A. deliciosa были получены мужскими растениями [22]. Также есть сообщение о морфологических различиях: в длине междоузлий, длине и ширине листа, и длине черешка среди растений-регенерантов, полученных из протопластов A. deliciosa [22]. Некоторые авторы пытались провести мутационную селекцию с помощью гамма-облучения пазушных и придаточных почек актинидии, однако этим методом улучшенных генотипов получено не было.…”

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Biotechnological and molecular methods in the study of the genus Actinidia Lindl. representatives

Matskiv,

Malyarovskaya

2023

SOH

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unclassified

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Biotechnological and molecular methods in the study of the genus Actinidia Lindl. representatives

Matskiv,

Malyarovskaya

2023

SOH

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SSR Marker–Based Genetic Diversity and Relationship Analyses of Stephania tetrandra S. Moore

Wu,

Bai,

Huang

et al. 2024

Plant Mol Biol Rep

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Stephania tetrandra S. Moore (family: Menispermaceae), a dioecious herbaceous vine and the only species in the subgenus Botryodiscia of the genus Stephania of the family Menispermaceae, is mainly distributed in hilly areas south of the Huaihe River in China and found in many provinces of China, showing a high genetic diversity. This paper aimed to study genetic diversity of and genetic relationship among individuals of S. tetrandra within China to provide a basis for evaluation, exploitation, and utilization of S. tetrandra by using simple sequence repeat (SSR) molecular markers. Our results show that effective products were amplified from the 26 screened SSR gene loci, a total of 183 alleles amplified (2–16 alleles amplified by each pair of primers). Among the 26 loci, 16 had a PIC value higher than 0.5, indicating a high level of polymorphism. For most of the loci, the number of effective alleles was lower than that of the observed alleles, and the observed heterozygosity was lower than the expected heterozygosity. The genetic differentiation coefficient (0.021–0.547) was lower than 0.05 (low level of genetic differentiation) for 7 loci and higher than 0.25 (very high level of genetic differentiation) for 2 loci, and had a value representing a medium level of genetic differentiation for the remaining 17 loci. The intra-population inbreeding coefficient had a positive value for 21 loci, suggesting the presence of inbreeding and homozygous excess. The gene flow value was bigger than 1, indicating that genetic drift and natural selection played an unimportant role in population genetic differentiation of S. tetrandra. Based on discriminant analysis of principal components and Bayesian Information Criterion, K-means clustering was performed on 620 samples. These samples were divided into 9 genetic clusters, whose similarity coefficients and genetic distances were 0.755–0.918 and 0.067–0.280, respectively, indicating that these clusters were highly similar and short-distanced. The Bayesian clustering analysis was implemented in the STRUCTURE software to analyze the genetic structure of S. tetrandra and it was found that the 620 samples could be clustered into 5 ancestor groups; the 9 clusters and 40 natural populations inherited genes from the 5 groups to varying degrees, but the proportion of genes inherited from the 5 groups by each cluster and natural population differed. S. tetrandra was characterized by the presence of population structure and pronounced genetic subdivision, which, together with the presence of gene flow, may indicate a relatively stable recent state of these populations.

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Protoplast isolation and genetically true-to-type plant regeneration from leaf- and callus-derived protoplasts of Albizia julibrissin

Rahmani

Pijut

Shabanian

2016

Plant Cell Tiss Organ Cult

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Protoplast Isolation and Culture of Woody Plants

Cited by 3 publications

References 28 publications

Biotechnological and molecular methods in the study of the genus Actinidia Lindl. representatives

Biotechnological and molecular methods in the study of the genus Actinidia Lindl. representatives

SSR Marker–Based Genetic Diversity and Relationship Analyses of Stephania tetrandra S. Moore

Protoplast isolation and genetically true-to-type plant regeneration from leaf- and callus-derived protoplasts of Albizia julibrissin

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