Protoscolicidal Effects and Acute Toxicity of Essential Oil and Methanolic Extract of Cuminum cyminum Seeds

Keyhani, Amir; Kareshk, Amir Tavakoli; Oliaei, Razieh Tavakoli; Mahmoudvand, Hossein

doi:10.12991/marupj.319204

Cited by 8 publications

(5 citation statements)

References 21 publications

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“…They revealed that C cyminum essential oil at a concentration of 50 and 25 μL/mL and the methanolic extract at concentrations of 100 and 50 mg/mL killed 100% protoscolices after exposure for 10 and 20 minutes, respectively. 28 In the present study, methanol extract of S perfoliata, at the concentration of 0.1, 0.2 and 0.4 mg/mL at different exposure periods showed significant scolicidal activity. At the end of the work there was no sufficient protoscolices to continue for more concentrated extract.…”

Section: F I G U R Ementioning

confidence: 46%

In vitro scolicidal effects of Sideritis perfoliata extract against Echinococcus granulosus

et al. 2021

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Cystic echinococcosis (CE) also known as hydatid disease is caused by the metacestode form of Echinococcus granulosus is a significant but neglected zoonotic parasitic infection all over the world. 1 The adult cestodes live in the small intestine of dogs which are primary host and also other carnivores. Oral uptake of larvae can form infection in intermediate hosts including cattle, sheep, pigs, horses and humans. 2 Cystic hydatid disease mainly affects the liver (50%-70% of cases) but can also develop in the lung (20%-30% of cases) and, less frequently, in the spleen, kidney, bone, brain, and other organs. 3 Currently, there are three options for the treatment of hydatid disease including chemotherapy, surgery and percutaneous drainage. Surgery is the most preferred option for treatment of hydatid cysts in humans. However, during surgery protoscolices are inevitably released into the body cavities and cause recurrence of the disease. 4,5

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Section: F I G U R Ementioning

confidence: 46%

In vitro scolicidal effects of Sideritis perfoliata extract against Echinococcus granulosus

et al. 2021

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“…Several plant extracts have been tested in vivo against CE and revealed high efficacy; however, only a few essential oils were used. The commonly tested essential oils against CE in vivo include carvacrol [ 59 ], β-myrcene [ 6 ] essential oils, Cuminum cyminum seeds [ 60 ], and Zataria multiflora [ 13 , 61 ], Zingiber officinale [ 43 ], Allium sativum [ 62 ], Punica granatum peel [ 10 ], and Ziziphora tenuior [ 63 ] plant extracts.…”

Section: Discussionmentioning

confidence: 99%

Eugenol Essential Oil and Nanoemulsion as Antihydatic Agents with Antifibrotic and Immunomodulatory Effects in Cystic Echinococcosis

et al. 2023

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Conventional scolicidal agents are still unsatisfactory in combating hydatid disease due to their low efficacy and increased drug side effects. Therefore, novel scolicides are required. This study aimed to evaluate the antihydatic and immunomodulatory effects of eugenol essential oil (Eug) and its nanoemulsion (Eug-NE) in cystic echinococcosis (CE). Eug and Eug-NE were administered orally to CE-infected rats and compared to albendazole (ABZ). Hydatid cyst development was assessed based on organ weight and hypertrophy indicators of the infected organs, along with a histopathological and histochemical evaluation of collagen content. The immunomodulatory effects of treatment on CE were evaluated by serum cytokine levels measurement of interferon-γ (IFN-γ) and interleukin (IL)-4 and immunohistochemical (IHC) analysis of signal transducer and activator of transcription 4 (STAT4) and GATA-binding protein 3 (GATA3) markers. Eug-NE was the most effective in reducing the cyst weights, organ weights, and hypertrophy indicators and improving histopathological lesions with reduced collagen content. Eug and Eug-NE significantly increased the IFN-γ levels and decreased the IL-4 levels, while IHC analysis demonstrated a significant reduction in STAT4 and GATA3 expression in all treated groups. Eug and Eug-NE demonstrated antihydatic and preventative effects, with a substantial decrease in liver fibrosis compared to that of ABZ. Besides their promising immunomodulatory effects, their good treatment response suggests their use as alternatives or complementary scolicidal agents in hydatid cyst treatment.

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“…Recently, N.sativa seeds oil has an effective antimicrobial activities considering their low coasty and good safety products (37). Agrawal et al (38) manifested significant N. sativa seed's oil antihelmintic activity against some pathogenic nematode and cestode.…”

Section: Discussionmentioning

confidence: 99%

Variations in the viability and macromolecules concentration of E. granulosus protoscolices isolated from ruminants consequence treatment with Nigella sativa seed's oil (In vitro study)

Yahya¹

2019

IJVS

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The aim of the present study was to investigate the scolicidal activity of Nigella sativa seed's oil concerning the viability in vitro and biomolecules content (carbohydrates, proteins and nucleic acids) of Echinococcus granulosus protoscolices. Protoscolices were aseptically aspirated from cysts of livers and lungs (naturally infected) which eradicated from goats and sheep that had been slaughtered at Mosul local abattoir / Nineveh / Iraq from September 2017 to May 2018. Various concentrations of N. sativa seed's oil (20 to 60 µl/ml) were applied on special time interval (5-40min). Viability of protoscolices was checked using vital stain (0.1% aqueous eosin). Concentration of carbohydrates, proteins and nucleic acids (DNA and RNA) were estimated after treating the protoscolices with LC 50 of the seed's oil (40 µl/ml) comparing with untreated group. It is revealed that mortality rate of protoscolices, those were treated for 10 minutes with 40 µl/ml and 60 µl/ml of Nigella sativa oil, were approximately 50% and 100% respectively. Viability % of protoscolices treated with 20% hypertonic saline solution for 5 minutes was 43%, whereas, 16% of protoscolices were viable when treated with 60 µl/ml of seed's oil at the same time. The experiments detect concentration and time-dependent scolicidal effect of N. sativa seed's oil on the E. granulosus protoscolices. Mean concentrations of carbohydrates, proteins and nucleic acids (DNA and RNA) were significantly higher at P≤ 0.05 in control group (62.6µg/ml, 31.0 mg/dl and (23.4 and 82.9 µg/ml, respectively) than that found in LC 50 treated protoscolices (58.3 µg/ml, 15.3 mg/dl and (18.19 and 64.48 µg/ml, respectively). The study showed that oil extract of N. sativa seeds has a significant (P≤ 0.05) clear impact in reducing viability of E. granulosus protoscolices, as well as, mean concentrations of its biomolecules which may open away for further experiments about scolicidal validity of N. sativa seeds oil in vivo.

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Protoscolicidal Effects and Acute Toxicity of Essential Oil and Methanolic Extract of Cuminum cyminum Seeds

Cited by 8 publications

References 21 publications

In vitro scolicidal effects of Sideritis perfoliata extract against Echinococcus granulosus

In vitro scolicidal effects of Sideritis perfoliata extract against Echinococcus granulosus

Eugenol Essential Oil and Nanoemulsion as Antihydatic Agents with Antifibrotic and Immunomodulatory Effects in Cystic Echinococcosis

Variations in the viability and macromolecules concentration of E. granulosus protoscolices isolated from ruminants consequence treatment with Nigella sativa seed's oil (In vitro study)

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