Prototheca Infections and Ecology from a One Health Perspective

Libisch, Balázs; Picot, Carine; Ceballos-Garzón, Andrés; Morávková, Monika; Klimešová, Marcela; Telkes, Gábor; Chuang, Shih-Te; Pape, Patrice Le

doi:10.3390/microorganisms10050938

Cited by 22 publications

(34 citation statements)

References 82 publications

(212 reference statements)

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“…Infected cows are a reservoir of Prototheca and, through the milk, a source of infection for other cows. Manure probably plays an important role in the dissemination of Prototheca species since the alga can be isolated from the intestine of the cow [36,37]. Prototheca mastitis can spread directly from cow to cow at milking time, or indirectly by contamination of the environment.…”

Section: Control Of Bovine Mastitis Due To Protothecamentioning

confidence: 99%

High Prevalence of Prototheca bovis Infection in Dairy Cattle with Chronic Mastitis in Ecuador

Huilca-Ibarra

Vasco-Julio

Ledesma

et al. 2022

Veterinary Sciences

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The genus Prototheca, a unicellular, non-photosynthetic, yeast-like microalgae, is a pathogen of concern for the dairy industry. It causes bovine mastitis that currently cannot be cured, and hence generates significant economic losses in milk production. In this study, for the first time in Ecuador, we identify Prototheca bovis as the etiologic agent of chronic mastitis in dairy cattle. Milk samples (n = 458) of cows with chronic mastitis were cultured on Sabouraud Dextrose Agar (SDA). Microscopy and cytB gene sequencing were used to identify Prototheca, whereby Prototheca bovis was isolated from 15.1% (n = 69) of the milk samples, one of the highest infection rates that can be found in the literature in a “non-outbreak” situation. No other Prototheca species were found. We were unable to isolate the alga from environmental samples. We showed that P. bovis was relatively resistant to disinfectants used to sterilize milking equipment on the cattle farms where it was isolated. We discuss how to avoid future infection and also hypothesize that the real prevalence of Prototheca infection in bovine mastitis is probably much higher than what was detected. We recommend a protocol to increase the diagnostic yield in the bacteriology laboratory.

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Section: Control Of Bovine Mastitis Due To Protothecamentioning

confidence: 99%

High Prevalence of Prototheca bovis Infection in Dairy Cattle with Chronic Mastitis in Ecuador

Huilca-Ibarra

Vasco-Julio

Ledesma

et al. 2022

Veterinary Sciences

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“…The disease occurs as a result of the invasion of the lacteous glands by pathogenic bacteria, followed by their reproduction in the mammary tissues ( Mushtaq et al ., 2017 ). The clinical symptoms of the cows with mastitis ( Libisch et al ., 2022 ):…”

Section: Introductionmentioning

confidence: 99%

Development of unconventional treatments for mastitis in dairy cattle

Kukeeva¹,

Abdrakhmanov²,

Akhmetov³

et al. 2023

Open Vet J

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Background: The increase in the intensity of livestock industries, by improving the technology of animal products manufacturing, largely depends on the correct organisation of herd reproduction and the intensity of using the biological capabilities of the animal organism. Various diseases, including such common diseases as mastitis, complicate the successful reproduction and growth of enterprise productivity. The widespread use of antibiotic-containing drugs for the treatment of mastitis creates a number of inevitable consequences for the body. The relevance of the study is due to the fact that the residual content of antibiotics in the collected milk after the course of treatment poses a serious danger to human health and reduces the quality of dairy products obtained from such milk. Aim: In this regard, the authors set the task of developing a new, antibiotic-free method of treating bovine mastitis. This paper is devoted to the problem of improving methods of treating the subclinical mastitis in dairy cattle by alternative methods during the interlactation period. Methods: The leading method for the study of this problem is an experimental method that allows to develop and test a veterinary homeopathic substance for the treatment of subclinical mastitis in cows during the interlactation period. Results: The paper presents materials on the typification of microflora in the milk of cows with subclinical mastitis and the effectiveness of a veterinary homeopathic substance developed by the authors. The use of the veterinary homeopathic substance provided a high therapeutic effect, did not cause side effects and complications in cows. Conclusion: The resulting veterinary substance was tested and introduced into the “Izhevskiy” natural complex of Akmola region as a new alternative method of treating subclinical mastitis in cows. Based on this substance, a drug for the treatment of mastitis will be developed and proposed for production.

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“…Metagenomic methods can overcome the limitations of culture-based approaches as the entire repertoire of resistance genes (the resistome) can be assessed in a sample without culturing single isolates of pre-selected species, which may cause a loss of relevant information [ 7 ]. Metagenomics may also contribute to the identification and characterization of non-culturable, difficult-to-culture, or slow growing microorganisms and be used for the tracking of genetic determinants of AMR or virulence [ 27 , 28 ]. Since shotgun metagenomic sequencing facilitates the detection of a wider range of ARGs in complex bacterial communities compared to conventional PCR-based approaches, it may contribute to the tracing of ARGs between different habitats and environments and to identifying potential routes of dissemination [ 7 , 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Detection of Acquired Antibiotic Resistance Genes in Domestic Pig (Sus scrofa) and Common Carp (Cyprinus carpio) Intestinal Samples by Metagenomics Analyses in Hungary

et al. 2022

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The aim of this study was metagenomics analyses of acquired antibiotic-resistance genes (ARGs) in the intestinal microbiome of two important food-animal species in Hungary from a One Health perspective. Intestinal content samples were collected from 12 domestic pigs (Sus scrofa) and from a common carp (Cyprinus carpio). Shotgun metagenomic sequencing of DNA purified from the intestinal samples was performed on the Illumina platform. The ResFinder database was applied for detecting acquired ARGs in the assembled metagenomic contigs. Altogether, 59 acquired ARG types were identified, 51 genes from domestic pig and 12 genes from the carp intestinal microbiome. ARG types belonged to the antibiotic classes aminoglycosides (27.1%), tetracyclines (25.4%), β-lactams (16.9%), and others. Of the identified ARGs, tet(E), a blaOXA-48-like β-lactamase gene, as well as cphA4, ampS, aadA2, qnrS2, and sul1, were identified only in carp but not in swine samples. Several of the detected acquired ARGs have not yet been described from food animals in Hungary. The tet(Q), tet(W), tet(O), and mef(A) genes detected in the intestinal microbiome of domestic pigs had also been identified from free-living wild boars in Hungary, suggesting a possible relationship between the occurrence of acquired ARGs in domestic and wild animal populations.

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Prototheca Infections and Ecology from a One Health Perspective

Cited by 22 publications

References 82 publications

High Prevalence of Prototheca bovis Infection in Dairy Cattle with Chronic Mastitis in Ecuador

High Prevalence of Prototheca bovis Infection in Dairy Cattle with Chronic Mastitis in Ecuador

Development of unconventional treatments for mastitis in dairy cattle

Detection of Acquired Antibiotic Resistance Genes in Domestic Pig (Sus scrofa) and Common Carp (Cyprinus carpio) Intestinal Samples by Metagenomics Analyses in Hungary

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