Prototypical anxiolytics do not reduce anxiety-like behavior in the open field in C57BL/6J mice

Thompson, Trey; Grabowski-Boase, Laura; Tarantino, Lisa M.

doi:10.1016/j.pbb.2015.03.011

Cited by 43 publications

(35 citation statements)

References 77 publications

(101 reference statements)

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“…Thus, it is tempting to suggest that SERT antagonism by cocaine reduces the potentially anxiogenic effect of being injected with a powerful psychoactivating agent. However, the interpretation of centre/surround measure as an anxiety measure has also been questioned due to the failure of mouse strains (particularly the C57BL/6 background of our SERT Met172 line) to be sensitive to prototypical anxiolytic drugs (Thompson et al, ). As the latter agents typically act via GABAergic mechanisms, it is possible that the broader actions of these agents may confound attempts to use pharmacology to attribute predictive validity to this measure.…”

Section: Discussionmentioning

confidence: 99%

Blockade of the 5‐HT transporter contributes to the behavioural, neuronal and molecular effects of cocaine

Simmler

Anacker

Levin

et al. 2017

British J Pharmacology

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Section: Discussionmentioning

confidence: 99%

Blockade of the 5‐HT transporter contributes to the behavioural, neuronal and molecular effects of cocaine

Simmler

Anacker

Levin

et al. 2017

British J Pharmacology

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“…Diazepam-induced increases in locomotion have been noted elsewhere, with other inbred strains of mice under other lighting conditions at doses below those that indicated behavioral sedation (Cole and Rodgers 1995; Rodgers et al 1992), including B6 mice (LaBuda and Fuchs 2001; Lepicard et al 2000). Additionally, several reports suggest that B6 mice may not be as sensitive to the anxiolytic effects of prototypical anxiolytic drugs, compared to other strains (Griebel et al 2000; Thompson et al 2015). …”

Section: Discussionmentioning

confidence: 99%

Evaluation of the neuroactive steroid ganaxolone on social and repetitive behaviors in the BTBR mouse model of autism

et al. 2015

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Rationale Abnormalities in excitatory/inhibitory neurotransmission are hypothesized to contribute to autism spectrum disorder (ASD) etiology. BTBR, an inbred mouse strain, displays social deficits and repetitive self-grooming, offering face validity to ASD diagnostic symptoms. Reduced GABAergic neurotransmission in BTBR suggests that GABAA receptor positive allosteric modulators (PAMs) could improve ASD-relevant BTBR phenotypes. The neuroactive steroid ganaxolone acts as a PAM, displaying anticonvulsant properties in rodent epilepsy models and an anxiolytic-like profile in the elevated plus-maze. Objectives We evaluated ganaxolone in BTBR and C57BL/6J mice in standardized assays for sociability and repetitive behaviors. Open field and anxiety-related behaviors were tested as internal controls and for comparison with the existing neuroactive steroid literature. Results Ganaxolone improved aspects of social approach and reciprocal social interactions in BTBR, with no effect on repetitive self-grooming, and no detrimental effects in C57BL/6J. Ganaxolone increased overall exploratory activity in BTBR and C57BL/6J in the open field, social approach, and elevated plus-maze, introducing a confound for the interpretation of social improvements. Allopregnanolone and diazepam similarly increased total entries in the elevated plus-maze, indicating that behavioral activation may be a general property of GABAA receptor PAMs in these strains. Conclusions Ganaxolone shows promise for improving sociability. In addition, ganaxolone, as well as other GABAA receptor PAMs, enhanced overall BTBR activity. The translational implications of specific sociability improvements and non-specific behavioral activation by ganaxolone in the BTBR model remains to be determined. Future studies to explore whether PAMs provide a novel profile with unique benefits for ASD treatment will be worthwhile.

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“…The above conflicting results were mostly obtained in unconditioned tests of anxiety, which have been reported to produce inconsistent results with a wide range of psychoactive compounds (Andrews et al, 2014;Cryan & Sweeney, 2011;Griebel & Holmes, 2013;Miczek & de Wit, 2008;Rodgers et al, 1995Rodgers et al, , 2002; Thompson et al, 2015). One of the major limitations of these tests is that they cannot be used for more Correspondence: Dr. A. Ennaceur, Sunderland Pharmacy School, University of Sunderland, Wharncliffe Street, Sunderland, SR1 3SD, UK.…”

Section: Introductionmentioning

confidence: 99%

Effects of chronic fluoxetine treatment on anxious behaviour of BALB/c mice in a 3-dimensional maze

Abuhamdah

Hussain

Chazot

et al. 2015

Stress

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Here we used a 3-dimensional (3D) maze, a modification of the radial maze, to assess the effects of treatment for two weeks with a single daily dose of fluoxetine (20 mg/kg, i.p.) on anxiety in male BALB/c mice. We examined whether anxiolytic effects of fluoxetine can be detected over three daily test sessions. We examined also whether repeated handling associated with chronic treatment interferes with effects of fluoxetine on anxiety responses. The 3D maze comprises nine arms, each connected to an upward inclined bridge radiating from a central platform. In this maze, BALB/c mice cross frequently into the bridges but avoid the arms. This avoidance is used as an index of anxiety. Two separate groups received once a day either saline (SALCH, n = 8) or fluoxetine (FLUCH, n = 8) for 14 days, and up to 30 min before the test during the subsequent 3 days. A third group received saline (SALAC, n = 8) 30 min before the test, once a day for 3 days. SALAC mice did not cross into the arms, and continued this avoidance over 3 sessions. SALCH mice avoided the arms in session 1 whereas FLUCH mice did cross into the arms, and like SALCH mice, increased number of crossings into and time on the arms in subsequent sessions. Fluoxetine evidently had an anxiolytic effect but only in the first session. These results indicate that handling experience decreased fear and anxiety in the mice, which may have masked the anxiolytic effect of fluoxetine in the second and third test sessions.

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Prototypical anxiolytics do not reduce anxiety-like behavior in the open field in C57BL/6J mice

Cited by 43 publications

References 77 publications

Blockade of the 5‐HT transporter contributes to the behavioural, neuronal and molecular effects of cocaine

Blockade of the 5‐HT transporter contributes to the behavioural, neuronal and molecular effects of cocaine

Evaluation of the neuroactive steroid ganaxolone on social and repetitive behaviors in the BTBR mouse model of autism

Effects of chronic fluoxetine treatment on anxious behaviour of BALB/c mice in a 3-dimensional maze

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