Protozoan Spirostomum ambiguum as a highly sensitive bioindicator for rapid and easy determination of water quality

Nałęcz-Jawecki, Grzegorz; Demkowicz-Dobrzainski, K.; Sawicki, J.

doi:10.1016/s0048-9697(05)80128-7

Cited by 22 publications

(14 citation statements)

References 7 publications

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“…With respect to abiotic factors, Volvic was the most suitable mineral water for culturing and 25°C was the correct temperature for its mass multiplication. The fact that light or darkness did not affect the survival of the ciliate agrees with the findings of Kidawa (1984) and Nalecz-Jaweski et al (1993) on Spirostomum ambiguum. Concerning the biotic factors, boiled wheat grains were well suited for culturing of the species, and the ciliates' multiplication rate was clearly correlated with the bacterial concentration in the culturing medium.…”

Section: Discussionsupporting

confidence: 91%

A New Low-Cost Microbiotest with the ProtozoanSpirostomum teres: Culture Conditions and Assessment of Sensitivity of the Ciliate to 14 Pure Chemicals

Twagilimana

Bohatier

et al. 1998

Ecotoxicology and Environmental Safety

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Section: Discussionsupporting

confidence: 91%

A New Low-Cost Microbiotest with the ProtozoanSpirostomum teres: Culture Conditions and Assessment of Sensitivity of the Ciliate to 14 Pure Chemicals

Twagilimana

Bohatier

et al. 1998

Ecotoxicology and Environmental Safety

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“…ambiguum . The finding that light or darkness does not seem to markedly influence the survival of the ciliates under laboratory conditions confirms the observations reported by Kidawa (1984Kidawa ( , 1987 and Nalecz-Jawecki et al . (1993) ; the positive role of dry leaves and adjuvant to other foods also corroborates the findings of the latter author.…”

Section: Discussionsupporting

confidence: 90%

“…Influence of type offood -Need for vegetal adjuvants Preliminary experiments were performed on the survival of the two ciliate strains SAW and SAB in glass petri dishes and in 1 .5 1 aquaria, in the Tyrod medium, an artificial freshwater used by the Polish scientists for short term maintenance of the ciliates (Nalecz-Jawecki et al ., 1993) . For the SAB strain, survival was also assessed in the natural water from the pond in Besse, of which a small volume was stored in the laboratory in plastic bottles at 4 °C and in darkness .…”

Section: Survival Experimentsmentioning

confidence: 99%

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A new low cost microbiotest with the freshwater ciliate protozoan Spirostomum ambiguum

1996

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This paper reports on preliminary experiments on the controlled culturing of the freshwater ciliated protozoan Spirostomum ambiguum, as a first step in the development of a new low cost microbiotest, to complement the limited battery of standardized cost-effective toxicity tests presently available, with a representative of unicellular biota.Three strains of S. ambiguum originating from France and Poland respectively, have been grown in natural as well as in reconstituted waters, on several types of inert diets and in different temperature and light conditions .The best results in terms of survival and reproduction of the ciliates, leading to a standard recipe for stock culturing, were obtained at 20 °C, in reconstituted Volvic water and on a diet of oat flakes supplemented with dried leave powder as vegetal adjuvant, with weekly renewal of the medium and food . An average generation time of 4 days was obtained in the latter set of stock culturing conditions .

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“…Spirotox uses a large ciliate protozoan-Spirostomum ambiguum-which is sensitive to many compounds, especially heavy metals. 10 The goal of the present investigation was to evaluate the possibility of applying Spirotox and Microtox tests in assessing the toxicity of extracts of medical devices.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of toxicity of medical devices using Spirotox and Microtox tests: I. Toxicity of selected toxicants in various diluents

Nałęcz-Jawecki¹,

Rudź²,

Sawicki³

1997

J. Biomed. Mater. Res.

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Significant effort has been directed toward developing in vitro alternatives, which can be the first step of toxicity analysis. Tissue culture assays are currently the most popular in vitro tests for evaluating acute toxicity. The possibility of applying two bioassays using microorganisms in assessing the toxicity of extracts of medical devices was investigated. The Microtox test system-a luminescent bacteria toxicity test-assesses changes in light output from a luminescent bacteria, Vibrio fischeri. Spirotox used a large ciliate protozoan: Spirostomum ambiguum. The most widely used extraction solvent, 0.9% NaCl, must be concentrated up to 2% for Microtox and diluted nine times for the Spirotox test.The organic solvents ethanol, DMSO, and polyethylene glycol 400 were not toxic in either test in concentrations of 1-2%. The toxicity of reference compounds Hg, Cd, Zn, Pb, and SDS was examined in various diluents. The sequence of toxicity of the tested compounds in Spirotox and Microtox was: Hg > Cd > Zn > Pb > SDS, and Hg > Pb = Zn > SDS > Cd, respectively. Addition of organic solvents changed the toxicity of compounds tested in 60% of Spirotox tests and only in 25% of Microtox tests. Changes were low, not exceeding 100% in almost all cases. No correlation was observed between diluent and toxicant in either bioassay.

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Protozoan Spirostomum ambiguum as a highly sensitive bioindicator for rapid and easy determination of water quality

Cited by 22 publications

References 7 publications

A New Low-Cost Microbiotest with the ProtozoanSpirostomum teres: Culture Conditions and Assessment of Sensitivity of the Ciliate to 14 Pure Chemicals

A New Low-Cost Microbiotest with the ProtozoanSpirostomum teres: Culture Conditions and Assessment of Sensitivity of the Ciliate to 14 Pure Chemicals

A new low cost microbiotest with the freshwater ciliate protozoan Spirostomum ambiguum

Evaluation of toxicity of medical devices using Spirotox and Microtox tests: I. Toxicity of selected toxicants in various diluents

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