Providing osteogenesis conditions to mesenchymal stem cells using bioactive nanocomposite bone scaffolds

Kim, Jung Ju; Jin, Guang Zhen; Yu, Hye Sun; Choi, Seong–Jun; Kim, Hae Won; Wall, Ivan

doi:10.1016/j.msec.2012.07.038

Cited by 16 publications

(13 citation statements)

References 36 publications

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“…This result confirms that PLA-BG composite scaffolds made from extruded filaments are not cytotoxic. Kim et al (2012) have shown that MSCs on PLA-BG composite exhibit higher cell viability after 3 days compared to pure PLA. Regarding structure compatibility, the fabricated strut-by-strut 3D-structures are well-known in TE and have already proven their potential on bone tissue scaffolds (Hollister et al, 2002;Detsch et al, 2008;Rottensteiner et al, 2014).…”

Section: Discussionmentioning

confidence: 97%

Polymer-Bioactive Glass Composite Filaments for 3D Scaffold Manufacturing by Fused Deposition Modeling: Fabrication and Characterization

Distler

Fournier

Grünewald

et al. 2020

Front. Bioeng. Biotechnol.

100

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Critical size bone defects are regularly treated by auto-and allograft transplantation. However, such treatments require to harvest bone from patient donor sites, with often limited tissue availability or risk of donor site morbidity. Not requiring bone donation, three-dimensionally (3D) printed implants and biomaterial-based tissue engineering (TE) strategies promise to be the next generation therapies for bone regeneration. We present here polylactic acid (PLA)-bioactive glass (BG) composite scaffolds manufactured by fused deposition modeling (FDM), involving the fabrication of PLA-BG composite filaments which are used to 3D print controlled open-porous and osteoinductive scaffolds. We demonstrated the printability of PLA-BG filaments as well as the bioactivity and cytocompatibility of PLA-BG scaffolds using pre-osteoblast MC3T3E1 cells. Gene expression analyses indicated the beneficial impact of BG inclusions in FDM scaffolds regarding osteoinduction, as BG inclusions lead to increased osteogenic differentiation of human adipose-derived stem cells in comparison to pristine PLA. Our findings confirm that FDM is a convenient additive manufacturing technology to develop PLA-BG composite scaffolds suitable for bone tissue engineering.

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Section: Discussionmentioning

confidence: 97%

Polymer-Bioactive Glass Composite Filaments for 3D Scaffold Manufacturing by Fused Deposition Modeling: Fabrication and Characterization

Distler

Fournier

Grünewald

et al. 2020

Front. Bioeng. Biotechnol.

100

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show abstract

“…Bone tissue engineering is a research area with notable potential in the realm of repair and regeneration of bone injuries. The tissue engineering approach involves the use of biodegradable scaffold systems to structurally support a target site while host cells and/or transplanted cell populations are induced by bioactive materials, growth factors, or other stimulatory systems to regenerate bone (Fujioka‐Kobayashi et al, ; Kim et al, ; Lin et al, ; Wu et al, , ). Thermoplastic polymer scaffolds have featured prominently within this field as the materials are relatively simple to process and are chosen on the basis of biodegradability and biocompatibility while providing desired mechanical characteristics (Armentano et al, ; Neuendorf et al, ; Rezwan et al, ).…”

Section: Introductionmentioning

confidence: 99%

Effects of scaffold architecture on mechanical characteristics and osteoblast response to static and perfusion bioreactor cultures

Bartnikowski

Klein

Melchels

et al. 2014

Biotech & Bioengineering

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Tissue engineering focuses on the repair and regeneration of tissues through the use of biodegradable scaffold systems that structurally support regions of injury while recruiting and/or stimulating cell populations to rebuild the target tissue. Within bone tissue engineering, the effects of scaffold architecture on cellular response have not been conclusively characterized in a controlled-density environment. We present a theoretical and practical assessment of the effects of polycaprolactone (PCL) scaffold architectural modifications on mechanical and flow characteristics as well as MC3T3-E1 preosteoblast cellular response in an in vitro static plate and custom-designed perfusion bioreactor model. Four scaffold architectures were contrasted, which varied in inter-layer lay-down angle and offset between layers, while maintaining a structural porosity of 60 ± 5%. We established that as layer angle was decreased (90° vs. 60°) and offset was introduced (0 vs. 0.5 between layers), structural stiffness, yield stress, strength, pore size, and permeability decreased, while computational fluid dynamics-modeled wall shear stress was increased. Most significant effects were noted with layer offset. Seeding efficiencies in static culture were also dramatically increased due to offset (∼ 45% to ∼ 86%), with static culture exhibiting a much higher seeding efficiency than perfusion culture. Scaffold architecture had minimal effect on cell response in static culture. However, architecture influenced osteogenic differentiation in perfusion culture, likely by modifying the microfluidic environment.

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“…Therefore, many attempts to improve the bioactivity have been made, including the incorporation of bone-bioactive ceramics or surface modication with hydrophilic materials and proteins. [11][12][13][14][15][16][17][18][19][20][21][22] Composite scaffolds composed of those biopolymers with bioactive ceramics such as hydroxyapatite (HA), tricalcium phosphate (TCP) and bioactive glasses have shown enhanced biological capacity for bone regeneration, including improved cell adhesion and osteoblastic differentiation. [14][15][16][17] Adhesive proteins including collagen and bronectin (FN) have also been coated or covalently bound onto the surface of synthetic polymers primarily to stimulate initial cell adhesion, spreading, migration and mitosis.…”

Section: Introductionmentioning

confidence: 99%

Tethering bi-functional protein onto mineralized polymer scaffolds to regulate mesenchymal stem cell behaviors for bone regeneration

Lee¹,

Park²,

Yun³

et al. 2013

J. Mater. Chem. B

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Providing osteogenesis conditions to mesenchymal stem cells using bioactive nanocomposite bone scaffolds

Cited by 16 publications

References 36 publications

Polymer-Bioactive Glass Composite Filaments for 3D Scaffold Manufacturing by Fused Deposition Modeling: Fabrication and Characterization

Polymer-Bioactive Glass Composite Filaments for 3D Scaffold Manufacturing by Fused Deposition Modeling: Fabrication and Characterization

Effects of scaffold architecture on mechanical characteristics and osteoblast response to static and perfusion bioreactor cultures

Tethering bi-functional protein onto mineralized polymer scaffolds to regulate mesenchymal stem cell behaviors for bone regeneration

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