Proximity-Dependent Inhibition of Growth of Mannheimia haemolytica by Pasteurella multocida

Bavananthasivam, Jegarubee; Dassanayake, Rohana P.; Kugadas, Abirami; Shanthalingam, Sudarvili; Call, Douglas R.; Knowles, Donald P.; Srikumaran, Subramaniam

doi:10.1128/aem.01119-12

Cited by 22 publications

(18 citation statements)

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“…These results are consistent with our previous in vitro demonstration that B. trehalosi and P. multocida inhibit the growth of M. haemolytica (Dassanayake et al 2010a;Bavananthasivam et al 2012). Our finding that 77% of the lung tissue samples that were culture-negative for M. haemolytica were positive by our PCR assay supports our hypothesis that culture-based methods do not accurately detect M. haemolytica in lung tissues from pneumonic bighorns.…”

Section: Discussionsupporting

confidence: 92%

“…We reasoned that detection of M. haemolytica by culture-based methods was hindered by the inhibition of its growth by B. trehalosi and P. multocida. This notion was supported by our recent finding that B. trehalosi and P. multocida inhibit the growth of M. haemolytica when cocultured in vitro in broth cultures (Dassanayake et al 2010a;Bavananthasivam et al 2012). We hypothesized that an assay more sensitive than the culture-based methods would detect M. haemolytica in pneumonic lung tissues more accurately.…”

Section: Introductionmentioning

confidence: 85%

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PCR ASSAY DETECTSMANNHEIMIA HAEMOLYTICAIN CULTURE-NEGATIVE PNEUMONIC LUNG TISSUES OF BIGHORN SHEEP (OVIS CANADENSIS) FROM OUTBREAKS IN THE WESTERN USA, 2009–2010

Shanthalingam

Goldy

Bavananthasivam

et al. 2014

Journal of Wildlife Diseases

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ABSTRACT:Mannheimia haemolytica consistently causes severe bronchopneumonia and rapid death of bighorn sheep (Ovis canadensis) under experimental conditions. However, Bibersteinia trehalosi and Pasteurella multocida have been isolated from pneumonic bighorn lung tissues more frequently than M. haemolytica by culture-based methods. We hypothesized that assays more sensitive than culture would detect M. haemolytica in pneumonic lung tissues more accurately. Therefore, our first objective was to develop a PCR assay specific for M. haemolytica and use it to determine if this organism was present in the pneumonic lungs of bighorns during the 2009-2010 outbreaks in Montana, Nevada, and Washington, USA. Mannheimia haemolytica was detected by the species-specific PCR assay in 77% of archived pneumonic lung tissues that were negative by culture. Leukotoxin-negative M. haemolytica does not cause fatal pneumonia in bighorns. Therefore, our second objective was to determine if the leukotoxin gene was also present in the lung tissues as a means of determining the leukotoxicity of M. haemolytica that were present in the lungs. The leukotoxin-specific PCR assay detected leukotoxin gene in 91% of lung tissues that were negative for M. haemolytica by culture. Mycoplasma ovipneumoniae, an organism associated with bighorn pneumonia, was detected in 65% of pneumonic bighorn lung tissues by PCR or culture. A PCR assessment of distribution of these pathogens in the nasopharynx of healthy bighorns from populations that did not experience an all-age die-off in the past 20 yr revealed that M. ovipneumoniae was present in 31% of the animals whereas leukotoxin-positive M. haemolytica was present in only 4%. Taken together, these results indicate that culture-based methods are not reliable for detection of M. haemolytica and that leukotoxin-positive M. haemolytica was a predominant etiologic agent of the pneumonia outbreaks of 2009-2010.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 85%

PCR ASSAY DETECTSMANNHEIMIA HAEMOLYTICAIN CULTURE-NEGATIVE PNEUMONIC LUNG TISSUES OF BIGHORN SHEEP (OVIS CANADENSIS) FROM OUTBREAKS IN THE WESTERN USA, 2009–2010

Shanthalingam

Goldy

Bavananthasivam

et al. 2014

Journal of Wildlife Diseases

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“…jejuni affects the microbiota or that constituents of the microbiota affect C. jejuni independent of the host, as has been observed for other pathogenic bacteria [80,81,82,83]. In conclusion, we observed that high density colonization of the cecum by C.…”

Section: Discussionsupporting

confidence: 83%

Campylobacter jejuni Colonization Is Associated with a Dysbiosis in the Cecal Microbiota of Mice in the Absence of Prominent Inflammation

et al. 2013

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Background Campylobacter jejuni causes enterocolitis in humans, but does not incite disease in asymptomatic carrier animals. To survive in the intestine, C. jejuni must successfully compete with the microbiota and overcome the host immune defense. Campylobacter jejuni colonization success varies considerably amongst individual mice, and we examined the degree to which the intestinal microbiota was affected in mice (i.e. a model carrier animal) colonized by C. jejuni at high relative to low densities.MethodsMice were inoculated with C. jejuni or buffer, and pathogen shedding and intestinal colonization were measured. Histopathologic scoring and quantification of mRNA expression for α-defensins, toll-like receptors, and cytokine genes were conducted. Mucosa-associated bacterial communities were characterized by two approaches: multiplexed barcoded pyrosequencing and terminal restriction fragment length polymorphism analysis.ResultsTwo C. jejuni treatments were established based on the degree of cecal and colonic colonization; C. jejuni Group A animals were colonized at high cell densities, and C. jejuni Group B animals were colonized at lower cell densities. Histological examination of cecal and colonic tissues indicated that C. jejuni did not incite visible pathologic changes. Although there was no significant difference among treatments in expression of mRNA for α-defensins, toll-like receptors, or cytokine genes, a trend for increased expression of toll-like receptors and cytokine genes was observed for C. jejuni Group A. The results of the two methods to characterize bacterial communities indicated that the composition of the cecal microbiota of C. jejuni Group A mice differed significantly from C. jejuni Group B and Control mice. This difference was due to a reduction in load, diversity and richness of bacteria associated with the cecal mucosa of C. jejuni Group A mice.ConclusionsHigh density colonization by C. jejuni is associated with a dysbiosis in the cecal microbiota independent of prominent inflammation.

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“…) and Pasteurella multocida (Bavananthasivam et al . ) have previously been described to inhibit M. haemolytica by proximity‐dependent mechanisms. Thus, bacterial competition has an important role in the respiratory tract and perhaps commensals could be used as probiotics to inhibit pathogenic bacteria.…”

Section: Introductionmentioning

confidence: 99%

Probiotic bacteria inhibit the bovine respiratory pathogenMannheimia haemolyticaserotype 1in vitro

Verdú

Subramanian

Timsit

et al. 2017

Lett Appl Microbiol

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A common method to control bovine respiratory disease (BRD) in feedlots is through mass medication with antibiotics upon cattle entry (i.e. metaphylaxis). Increasingly, antimicrobial resistance in BRD bacterial pathogens has been observed in feedlots, which may have important implications for cattle health. In this study, probiotic strains were shown to adhere to bovine respiratory cells and inhibit the BRD pathogen M. haemolytica serotype 1 through competition and displacement. Probiotics may therefore offer a mitigation strategy to reduce BRD bacterial pathogens, in place of metaphylactic antimicrobials.

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Proximity-Dependent Inhibition of Growth of Mannheimia haemolytica by Pasteurella multocida

Abstract: ABSTRACTMannheimia haemolytica,Pasteurella multocida, andBibersteinia trehalosihave been identified in the lungs of pneumonic bighorn sheep (BHS;Ovis canadensis). Of these pathogens, Show more

Cited by 22 publications

References 30 publications

PCR ASSAY DETECTSMANNHEIMIA HAEMOLYTICAIN CULTURE-NEGATIVE PNEUMONIC LUNG TISSUES OF BIGHORN SHEEP (OVIS CANADENSIS) FROM OUTBREAKS IN THE WESTERN USA, 2009–2010

PCR ASSAY DETECTSMANNHEIMIA HAEMOLYTICAIN CULTURE-NEGATIVE PNEUMONIC LUNG TISSUES OF BIGHORN SHEEP (OVIS CANADENSIS) FROM OUTBREAKS IN THE WESTERN USA, 2009–2010

Campylobacter jejuni Colonization Is Associated with a Dysbiosis in the Cecal Microbiota of Mice in the Absence of Prominent Inflammation

Probiotic bacteria inhibit the bovine respiratory pathogenMannheimia haemolyticaserotype 1in vitro

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