Prunetin signals via G-protein-coupled receptor, GPR30(GPER1): Stimulation of adenylyl cyclase and cAMP-mediated activation of MAPK signaling induces Runx2 expression in osteoblasts to promote bone regeneration

Khan, Kainat; Pal, Subhashis; Yadav, Manisha; Maurya, Rakesh; Trivedi, Arun Kumar; Sanyal, Sabyasachi; Chattopadhyay, Naibedya

doi:10.1016/j.jnutbio.2015.07.021

Cited by 49 publications

(33 citation statements)

References 47 publications

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“…Cell communication genes can provide information reflecting communication between cells, between cells and the extracellular matrix, and between cells and molecules. The transforming growth factor β signaling pathway (TGF‐β), mitogen‐activated protein kinase signaling pathway (MAPK) and wnt sign‐ aling pathway (Wnt) were all selected because they play important roles in the osteogenic differentiation of ADSCs (Khan et al, ; Majidinia, Sadeghpour, & Yousefi, ) (Tables and ).…”

Section: Discussionmentioning

confidence: 99%

Differentiated adipose‐derived stem cell cocultures for bone regeneration in RADA16‐I in vitro

Yang

Hong

Liu

et al. 2018

Journal Cellular Physiology

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Craniofacial defects can cause morbidness. Adipose-derived stem cells (ADSCs) have shown great promise for osteogeneration and vascularization; therefore cocultures of differentiated ADSCs are explored to increase bone and vessel formation. In this study, ADSCs were induced into osteogenic ADSCs (os-ADSCs) and endothelial ADSCs (endo-ADSCs) cells, which were then cocultured in variable proportions (os-ADSCs/endo-ADSCs = 2:1, 1:1, 1:2). The os-ADSCs in a ratio of 1:1 expressed more ALP, RUNX2 and COL-I, whereas VEGF, vWF and CD31 were upregulated in the endo-ADSCs of this group. Next generation RNA sequencing (RNA-seq) was performed to evaluate the molecular mechanisms of cocultured ADSCs. The os-ADSCs and endo-ADSCs interacted with each other during osteogenic and angiogenic differentiation, especially at the ratio of 1:1, and were regulated by vascular-related genes, cell-mediated genes, bone-related genes and the transforming growth factor β signaling pathway (TGF-β), mitogen-activated protein kinase signaling pathway (MAPK) and wnt signaling pathway (Wnt). Angptl4, apoe, mmp3, bmp6, mmp13 and fgf18 were detected to be up-regulated, and cxcl12 and wnt5a were down-regulated. The results showed that the gene expression levels were consistent with that in RNA-seq. The cells were then seeded into self-assembling peptide RADA16-I scaffolds as cocultures (1:1) and monocultures (ADSCs, os-ADSCs, endo-ADSCs). The results showed that the cells of all groups grew and proliferated well on the scaffolds, and the cocultured group exhibited better osteogeneration and vascularization. In conclusion, cocultured os-ADSCs and endo-ADSCs at the ratio of 1:1 showed strong osteogenic and angiogenic differentiation. There is a great potential for osteogenesis and vascularization by 3D culturing cells in a 1:1 ratio in self-assembling peptide RADA16-I scaffolds, which requires evaluation for bone regeneration in vivo.

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Section: Discussionmentioning

confidence: 99%

Differentiated adipose‐derived stem cell cocultures for bone regeneration in RADA16‐I in vitro

Yang

Hong

Liu

et al. 2018

Journal Cellular Physiology

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“…Therefore, the noticed decrease of LPS-triggered NO production caused by extracts is due to their capacity to limit Nos2 gene transcription, hypothetically by interfering with pro-inflammatory transcription factors such as nuclear factor-κB (NF-κB) and signal transducer and activator of transcription 1 (STAT-1). In fact, the flavonoids genistein, prunetin, biochanin A derivatives, and kampferol have been shown to inhibit NO production by decreasing the activation of NF-κB signaling pathway [15,16,[27][28][29]. When analyzing the effects over Ptgs2 transcription it was found that significant decreases are only obtained by treatment with extracts from roots ( Figure 4).…”

Section: [μG/ml] Figmentioning

confidence: 99%

Flavonoid Profile of the Genista tridentata L., a Species Used Traditionally to Treat Inflammatory Processes

et al. 2020

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Ethnopharmacological surveys on Portuguese flora reveal that Genista tridentata L. is a shrub used in traditional medicine for the treatment of various inflammation-related health problems, although scientific support of its benefits is still necessary. In order to establish the anti-inflammatory potential of G. tridentata and support its traditional use, ethanolic extracts of three sections of the plant (root, stem, and leaves) were subjected to in vitro evaluation of anti-inflammatory activity using lipopolysaccharide (LPS)-stimulates macrophages as an inflammation model. Simultaneously, we also aimed to establish the extracts’ flavonoids profile. The ethanolic extracts, obtained by Soxhlet extraction, profile of the three sections confirmed their richness in flavonoids, being three prenylated flavonoids isolated and characterized in the root, including a new natural compound, the 3-methoxymundulin. The extracts from the three plant sections showed strong antioxidant activity at the cellular level and significantly inhibit the LPS-triggered NO production by downregulating Nos2 gene transcription and consequently iNOS expression. Additionally, root and stem extracts also decreased the LPS-induced transcription of the pro-inflammatory genes Il1b, Il6, and Ptgs2. Thus, the results support the anti-inflammatory properties attributed to G. tridentate preparations. Relevantly, the roots of the shrub, plant part not used, is an unexplored source of compounds with pharmacological and nutraceutical value.

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“…Given that polyphenols have also been demonstrated to have estrogen-like biological activities, it can be argued that they can modulate the estrogen-dependent pathway by acting as partial agonists and/or antagonists of the estrogen receptor (ER) in a tissue type and ligand concentration-dependent manner [79]. In this context, they do target the classical ER pathway, by binding to the ER and thus activating the ER-dependent gene transcription leading to inhibition of bone resorption and stimulation of osteoblastic bone formation [37,80,81], but they are also able to exert beneficial effects on bone system through the non-classical estrogen pathway, thus eliciting expression of the main osteoblast differentiation genes [82][83][84][85].…”

Section: Polyphenolsmentioning

confidence: 99%

Potentials of Polyphenols in Bone-Implant Devices

Torre¹,

Iviglia²,

Cassinelli³

et al. 2018

Polyphenols

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Knowledge of bioactive plant-derived polyphenols is growing to such an extent that science interest is looking at development of different applications in regenerative medicine through new and state-of-the-art tissue engineering technologies. Due to their well-established and demonstrated antioxidant and anti-inflammatory beneficial properties, polyphenols have been extensively investigated to the extent that they provide benefits to different pathological conditions, including cardiovascular and bone diseases, neurodegenerative disorders, and cancer. By taking into account the main molecular pathways of polyphenols' action, we want to focus this chapter on applications of polyphenols in boneimplant devices. In particular, results of polyphenols' effects on bone cells and tissues following local delivery from innovative biomaterials will be discussed, together with preliminary in vivo tests. Purpose of the dissertation is to provide the reader new insights into knowledge of polyphenols not only regarding the different molecular mechanisms involved in their action but also the biological responses deriving from local applications.

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Prunetin signals via G-protein-coupled receptor, GPR30(GPER1): Stimulation of adenylyl cyclase and cAMP-mediated activation of MAPK signaling induces Runx2 expression in osteoblasts to promote bone regeneration

Cited by 49 publications

References 47 publications

Differentiated adipose‐derived stem cell cocultures for bone regeneration in RADA16‐I in vitro

Differentiated adipose‐derived stem cell cocultures for bone regeneration in RADA16‐I in vitro

Flavonoid Profile of the Genista tridentata L., a Species Used Traditionally to Treat Inflammatory Processes

Potentials of Polyphenols in Bone-Implant Devices

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