PsAA9A, a C1-specific AA9 lytic polysaccharide monooxygenase from the white-rot basidiomycete Pycnoporus sanguineus

Abstract

Woody biomass represents an important source of carbon on earth and its global recycling is highly dependent on Agaricomycetes fungi. White-rot basidiomycetes are a very important group in this regard, as they possess a large and diverse enzymatic repertoire for biomass decomposition. Among these enzymes, the recently discovered lytic polysaccharide monooxygenases (LPMOs) have revolutionized biomass processing with their novel oxidative mechanism of action. The strikingly high representation of LPMOs in fungal genomes raises the question of their functional versatility. In this work, we studied an AA9 LPMO from the white-rot basidiomycete Pycnoporus sanguineus, PsAA9A. Successfully produced as a recombinant secreted protein in Pichia pastoris, PsAA9A was found to be a C1-specific LPMO active on cellulosic substrates, generating native and oxidized cello-oligosaccharides in the presence of an external electron donor. PsAA9A boosted cellulolytic activity of glysoside hydrolases from families GH1, GH5, and GH6.This study serves as a starting point towards understanding the functional versatility and biotechnological potential of this enzymatic family, highly represented in wood decay fungi, in Pycnoporus genus. KEY POINTSPsAA9A is the first AA9 from P. sanguineus to be characterized. PsAA9A has activity on cellulose, producing C1-oxidized cello-oligosaccharides. Boosting activity with GH1, GH5 and GH6 was proven. Manuscript with marked revisionsClick here to access/download;Manuscript;Manuscript with title page Revised.docx Click here to view linked References EC, NCB and SW conceived and designed research. MG, ML and FS conducted experiments. PV and AC contributed analytical tools and analyzed data. MG wrote the manuscript. All authors read and approved the manuscript.

Genome analysis of the esca-associated Basidiomycetes Fomitiporia mediterranea, Fomitiporia polymorpha, Inonotus vitis, and Tropicoporus texanus reveals virulence factor repertoires characteristic of white-rot fungi

“…This family confers abilities related to the degradation of benzoate residues, which is a common plant defense compound (Podobnik et al 2008;Lah et al 2011;Jawallapersand et al 2014). Similarly, the CBM1, AA9, and AA2 CAZymes, higher in white-rot fungi and the newly assembled genomes, are involved in lignocellulose substrate degradation (Lehtiö et al 2003;Liu et al 2017;Garrido et al 2020), suggesting these enzymes may play a crucial role in the activity of the white-rot fungi, consistent with previous reports by other researchers (Liu et al 2017;Wu et al 2020;Li et al 2019;Miyauchi et al 2020;Levasseur et al 2014;Floudas et al 2020). Additionally, researchers have reported high correlation between white-rot species and the families CBM1, GH7 and GH6 (Riley et al 2014;Floudas et al 2015Floudas et al , 2012.…”

Potentials of Biomass Waste Valorization: Case of South America

“…In recent years, the search and characterization of auxiliary enzymatic activities for biomass conversion have attracted the attention of the scientific community. In this context, Garrido and collaborators (2020) from Argentina cloned and characterized a recombi-nant secreted AA9 LPMO from the white-rot basidiomycete Pycnoporus sanguineus in the model yeast Pichia pastoris [97]. The synthesized enzyme boosted the activity of glycoside hydrolases from families GH1, GH5 and GH6, providing a clue to the versatility of LPMOs.…”