Pseudidiomarina aestuarii sp. nov., a marine bacterium isolated from shallow coastal seawater

Abstract

A non-motile, rod-shaped and aerobic marine bacterium, designated strain KYW314 T , was isolated from seawater collected from the South Sea, Republic of Korea. Cells were Gramnegative, catalase-and oxidase-positive and had proteolytic activity. The major fatty acids were iso-C 15 : 0 (17.2 %), C 16 : 0 (11.1 %), iso-C 17 : 0 (10.5 %) and C 18 : 1 v7c (10.0 %). The DNA G+C content was 56.4 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain KYW314 T formed a lineage within the genus Pseudidiomarina (95.4-97.3 % sequence similarity) and a distinct branch within the clade containing Pseudidiomarina taiwanensis PIT1 T and Pseudidiomarina sediminum c121 T . Phenotypic characteristics could distinguish strain KYW314 T from members of the genus Pseudidiomarina. On the basis of the data presented, strain KYW314 T represents a novel species, for which the name Pseudidiomarina aestuarii sp. nov. is proposed. The type strain is KYW314 T (5KCTC 22740 T 5JCM 16344 T ).A study of Alteromonas-like bacteria by Ivanova et al. (2004) resulted in the proposal of the family Idiomarinaceae, on the basis of phylogeny and signature nucleotide positions in the 16S rRNA gene. Members of the family Idiomarinaceae are unique among Alteromonas-like bacteria in possessing a high content of iso-branched fatty acids (Ivanova et al., 2004). The family Idiomarinaceae comprises two recognized genera: Idiomarina (Ivanova et al., 2000) as the type genus and Pseudidiomarina (Jean et al., 2006). Several species of the genus Pseudidiomarina are non-motile, while all species of the genus Idiomarina are motile by means of flagella.At the time of writing, the genus Pseudidiomarina comprises eight species, with Pseudidiomarina taiwanensis as the type species (Jean et al., 2006(Jean et al., , 2009Hu & Li, 2007;Wu et al., 2009). Members of the genus Pseudidiomarina have been isolated from saline habitats with a wide range of salinities such as coastal and oceanic waters, seashore sands, coastal sediment and solar salterns (Jean et al., 2006(Jean et al., , 2009Kwon et al., 2006;Yoon et al., 2007;Hu & Li, 2007;Wu et al., 2009). During a survey of the diversity of heterotrophic marine bacteria, a bacterial strain, KYW314 T , was recovered from seawater samples collected from the shallow coastal region of Gwangyang Bay in the south of the Republic of Korea (34 u 519 N 128 u 479 E) in July 2008. Strain KYW314 T was isolated from seawater using the standard dilution-plating technique. Isolation was achieved using marine agar 2216 (MA; BD) (Yang et al., 2006) at 30 u C for 7 days. The isolate was routinely cultured on MA and maintained at -80 u C as a suspension in marine broth (MB; BD) containing glycerol (20 %, w/v).DNA preparation and PCR amplification and sequencing of the 16S rRNA gene were carried out as described by Chun & Goodfellow (1995). Identification of phylogenetic neighbours and calculation of pairwise 16S rRNA gene sequence similarities were achieved using the EzTaxon server (http:// www.eztaxon.org/; Chun et al., 2007). ...

Idiomarina planktonica sp. nov., isolated from a saline lake

“…The enzyme activities detected by the API ZYM system and the carbon sources utilized by strain TS-T11 T and the three reference type strains are specified in both and the species description. Almost all the results obtained in this study for the reference type strains were consistent with those originally described (Jean et al , 2006; Park et al , 2010; Yoon et al , 2007), with the exception of leucine arylamidase and trypsin activity and hydrolysis of gelatin, l -arginine and urea for I. aestuarii JCM 16344 T (Park et al , 2010). These experiments were repeated several times and the same results were obtained, suggesting that these properties may be variable in I. aestuarii .…”

“…The fatty acid profiles of strain TS-T11 T and the three reference type strains are shown in . The cellular fatty acid profiles obtained in this study for the reference type strains were similar to those originally reported (Jean et al , 2006; Park et al , 2010; Yoon et al , 2007) and reported in other publications (Chen et al , 2012; Song et al , 2013; Taborda et al , 2009) in terms of major fatty acids. All these strains contained iso-C 15 : 0 , iso-C 17 : 0 and iso-C 17 : 1 ω9 c as major fatty acids, although in different proportions, which might be due to the different culture conditions.…”

Genome-Based Analysis Reveals the Taxonomy and Diversity of the Family Idiomarinaceae

“…The description is identical to that given for Pseudidiomarina aestuarii by Park et al (2010) . Additional characteristics are as follows: the polar lipids comprise PE, PG, DPG, and six uncharacterized PLs (PL1-PL5, PL12); hydrolysis of urea, gelatin, L -arginine, and the enzyme activities of leucine arylamidase and trypsin in API ZYM tests are variable ( Zhong et al, 2014 ).…”

Aliidiomarina haloalkalitolerans sp. nov., a marine bacterium isolated from coastal surface seawater

“…The major cellular fatty acids present are iso-C 15:0 and iso-C 17:0 (Ivanova et al 2004;Jean et al 2006) and the major isoprenoid quinone is ubiquinone 8 (Q-8) (Taborda et al 2009). Members of the family Idiomarinaceae have been isolated from variety of saline habitats, such as deep sea waters (Ivanova et al 2000), surface sea waters (Brettar et al 2003;Huang et al 2011;Wang et al 2011;Wu et al 2009), submarine hydrothermal fluids (Donachie et al 2003), estuarine waters (Jean et al 2006;Park et al 2010), coastal sediments (Hu and Li 2007), seashore sand (Kwon et al 2006), solar salterns (Taborda et al 2009;Yoon et al 2007) and inland hypersaline wetlands (Martínez-Cánovas et al 2004). The family Idiomarinaceae currently comprises the genera Aliidiomarina and Idiomarina as species originally assigned to the genus Pseudidiomarina were reassigned to the genus Idiomarina by Taborda et al (2009) as these two genera can not be differentiated on the basis of the phenotypic characteristics.…”