This paper presents a pseudo-differential transconductor circuit. Operational Transconductance Amplifier (OTA) is a standout amongst the most functional and major circuit elements within the analog and mixed-signal circuit style. It is additionally one of the more intricate cells to plan. Due to the rising performance of new generation MOS transistors, the complexity of integrated circuit is continuously increasing with time. The reduction in component sizing is one of the main reasons for integrating millions of transistors into a single chip. There is a great demand for battery powered equipment like a laptop, wireless communication, and implantable devices. In all these devices, it is essential to maintain low power dissipation to achieve good battery life and weight. The main feature of the research is to design a pseudodifferential transconductor circuit for a low supply voltage application with the targeted gain greater than 20 dB using Mentor Graphics software. The designs are done in Pyxis Schematic and Pyxis Layout using eldo platform for simulation to simulate the functionality of the transconductor circuit. From the postlayout simulation, with supply voltage of 1.2 V, the gain of 30 dB with cut-off frequency of 398 kHz has been achieved.