Pseudo-outbreak of Mycobacterium gordonae: usefulness of randomly amplified polymorphic DNA analysis to assess the clonality of the isolates

Esteban, Jaime; Fernández-Roblas, R.; Ortíz, Alberto; García-Cía, José I.

doi:10.1111/j.1469-0691.2006.01450.x

Cited by 8 publications

(7 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4 Lastly, environmental contamination may represent an uncharacterized, but potentially important source of our M. gordonae, Mycobacterium terrae complex, Mycobacterium mucogenicum, and Mycobacterium scrofulaceum isolate reports. 4,30 This was the first population-based study of NTM prevalence using reports of isolation in the Southeastern U.S.; it includes a more racially diverse population than studies conducted in many other geographic sites. In addition, this study utilized reports from public and private clinical laboratories, collected demographic information about patients and reported the anatomic source and site of NTM isolation.…”

Section: Discussionmentioning

confidence: 99%

Epidemiology of nontuberculous mycobacteria isolations among central North Carolina residents, 2006–2010

Smith

Ghio

Stout

et al. 2016

Journal of Infection

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Epidemiology of nontuberculous mycobacteria isolations among central North Carolina residents, 2006–2010

Smith

Ghio

Stout

et al. 2016

Journal of Infection

View full text Add to dashboard Cite

“…RAPD typing showed that these isolates were identical and different from unrelated control strains. Environmental sampling in the laboratory did not yield M. gordonae, but after sterilization or replacement of all consumables and reagents, no more related M. gordonae isolates were detected (528). Similarly, a pseudo-outbreak of M. terrae was reported in Philadelphia, PA, where a laboratory noted that 14 cultures handled in a 6-day period were positive; all were from AFB-negative clinical samples and yielded growth only with the ESP liquid medium system but not on simultaneously inoculated Lowenstein-Jensen slants.…”

Section: Molecular Typing Of Nontuberculous Mycobacteriamentioning

confidence: 90%

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

et al. 2016

View full text Add to dashboard Cite

SUMMARYMolecular typing has revolutionized epidemiological studies of infectious diseases, including those of a mycobacterial etiology. With the advent of fingerprinting techniques, many traditional concepts regarding transmission, infectivity, or pathogenicity of mycobacterial bacilli have been revisited, and their conventional interpretations have been challenged. Since the mid-1990s, when the first typing methods were introduced, a plethora of other modalities have been proposed. So-called molecular epidemiology has become an essential subdiscipline of modern mycobacteriology. It serves as a resource for understanding the key issues in the epidemiology of tuberculosis and other mycobacterial diseases. Among these issues are disclosing sources of infection, quantifying recent transmission, identifying transmission links, discerning reinfection from relapse, tracking the geographic distribution and clonal expansion of specific strains, and exploring the genetic mechanisms underlying specific phenotypic traits, including virulence, organ tropism, transmissibility, or drug resistance. Since genotyping continues to unravel the biology of mycobacteria, it offers enormous promise in the fight against and prevention of the diseases caused by these pathogens. In this review, molecular typing methods forMycobacterium tuberculosisand nontuberculous mycobacteria elaborated over the last 2 decades are summarized. The relevance of these methods to the epidemiological investigation, diagnosis, evolution, and control of mycobacterial diseases is discussed.

show abstract

“…RAPD has been claimed as a technique with low reproducibility, but we have the experience that, if strict conditions and criteria are followed, the obtained results can be considered useful for discrimination purposes (39)(40)(41). This technique also has the advantage of being easy to perform without complex equipment, such as that needed for other reference techniques like PFGE or complete-genome sequencing, so it can be performed in mediumsize laboratories like ours.…”

Section: Discussionmentioning

confidence: 99%

Detection of Polyclonality among Clinical Isolates from Prosthetic Joint Infections

et al. 2015

View full text Add to dashboard Cite

Prosthetic joint infection (PJI) is an increasingly important health concern in the Western world due to the rising number of joint arthroplasties. Although most infections are considered to be monomicrobial, the introduction of sonication procedures has led to an increase in the detection of polymicrobial infections. To date, no published studies have investigated the presence of different clones of the same species in the infected patient. The objective of this study was to analyze whether the phenomenon of polyclonality, or the appearance of different clones in the same sample, occurs in PJI. Bacteria isolated by sonication of the retrieved implant from patients with theoretically monomicrobial PJI were included in the study. Two techniques (random amplified polymorphic DNA [RAPD] and matrix-assisted laser desorption ionization-time of flight [MALDI-TOF] mass spectrometry) were used to determine the presence of several clones in the same sample. Results were analyzed to determine bacterial species and infection type (acute versus chronic). RAPD showed a predominance of polyclonal cases (16 of 19). However, when performing the analysis with MALDI-TOF, all cases were shown to be polyclonal. We were unable to establish any relationship between the two methodologies. Polyclonality is a common phenomenon in acute and chronic PJI. Further studies are needed to establish the potential implications of this phenomenon on patient outcomes. P rosthetic joint replacement, or arthroplasty, is a surgical procedure that has improved the quality of life for many people around the world, providing pain relief and improved functionality to limbs (1-3). However, 10% of all patients who undergo this operation develop complications at some point in their lives; although it is not the most common, infection is one of the most significant of these complications, having an incidence of 1% to 3% (1, 3, 4). The microorganisms that cause most cases of prosthetic joint infection (PJI) are those belonging to the genus Staphylococcus (60% of cases), of which infections caused by Staphylococcus aureus constitute 25%. Gram-negative organisms (Enterobacteriaceae, Pseudomonas aeruginosa, and other species) represent a smaller proportion of cases (10%) (1, 2, 5). Notably, up to 20% to 25% of PJIs are polymicrobial infections (1-3).When the growth conditions of the bacteria causing PJI become hostile, as may occur during antibiotic therapy, a coping mechanism known as spontaneous random hypermutation occurs as the bacteria attempt to overcome the unfavorable environment (6). This results in the development of polyclonality or the appearance of different clones in the same sample or environment (6). Polyclonality can also occur when the patient is infected with different clones from the same section of skin, which may occur either during surgery or afterwards and is likely a more common phenomenon (7). Several reports have found polyclonality in monomicrobial infections among isolates of small-colony variant staphylococci, which present phenotyp...

show abstract

Pseudo-outbreak of Mycobacterium gordonae: usefulness of randomly amplified polymorphic DNA analysis to assess the clonality of the isolates

Cited by 8 publications

References 15 publications

Epidemiology of nontuberculous mycobacteria isolations among central North Carolina residents, 2006–2010

Epidemiology of nontuberculous mycobacteria isolations among central North Carolina residents, 2006–2010

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

Detection of Polyclonality among Clinical Isolates from Prosthetic Joint Infections

Contact Info

Product

Resources

About