Pseudoalteromonas xiamenensis sp. nov., a marine bacterium isolated from coastal surface seawater

Abstract

Pseudoalteromonas xiamenensis sp. nov., a marine bacterium isolated from coastal surface seawater A Gram-negative, oxidase-and catalase-positive, rod-shaped, non-spore-forming, motile, aerobic bacterium, designated Y2 T , was isolated from surface seawater of Yundang Lake, Xiamen, China. The strain was able to grow in the presence of 0.5-6.0 % NaCl (optimum 1.0-1.5 %), at pH 5-10 (optimum pH 8) and at 10-40 6C (optimum 25 6C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain Y2 T belongs to the genus Pseudoalteromonas, with the highest sequence similarity of 94.9 % to Pseudoalteromonas tunicata D2 T ; within the genus Pseudoalteromonas, it showed the lowest similarity of 92.8 % to Pseudoalteromonas denitrificans ATCC 43337 T . The G+C content of the chromosomal DNA of strain Y2 T was 45.1 mol%. The predominant fatty acids were summed feature 3 (C 16 : 1 v6c and/or C 16 : 1 v7c), C 16 : 0 , C 12 : 0 3-OH and summed feature 8 (C 18 : 1 v6c and/or C 18 : 1 v7c). The only respiratory quinone detected was Q-8. Based on the phylogenetic and phenotypic characteristics, strain Y2 T represents a novel species of the genus Pseudoalteromonas, for which the name Pseudoalteromonas xiamenensis sp. nov. is proposed; the type strain is Y2 T (5CGMCC 1.12157 T 5JCM 18779 T ).

Pseudoalteromonas fenneropenaei sp. nov., a marine bacterium isolated from sediment of a Fenneropenaeus chinensis pond

“…The respiratory quinones were Q-8 (95.3 %) and Q-9 (3.6 %), which were similar to those of the species P. xiamenensis, P. arabiensis and P. lipolytica (Zhao et al, 2014;Matsuyama et al, 2013;Xu et al, 2010). Polar lipids were extracted and analysed as described by Minnikin et al (1984) and Komagata & Suzuki (1987).…”

“…Biochemical characteristic and enzyme activity tests were carried out using API ZYM, API 20 NE and API 20E test kits (bioM erieux) according to the manufacturer's instructions except that the NaCl concentration was adjusted to 1.0 % in all tests. Pseudoalteromonas xiamenensis MCCC 1A06494 (Zhao et al, 2014), Pseudoalteromonas ulvae MCCC 1A00555 (Egan et al, 2001) and Pseudoalteromonas tunicata MCCC 1A06495 (Holmström et al, 1998), obtained from the Marine Culture Collection of China (MCCC, Xiamen, China), were tested at the same time for comparison. The results are shown in the species description and Table 1.…”

Pseudoalteromonas aestuariivivens sp. nov., isolated from a tidal flat

“…S2). The DNA G+C content of strain DB-2 T was 54.9¡0.2 mol%, a value higher than those reported for species of the genus Pseudoalteromonas (Table 1; Ivanova et al, 2002;Matsuyama et al, 2014;Zhao et al, 2014).…”

“…In the neighbour-joining, maximumlikelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences, strain DB-2 T fell within the clade comprising the type strains of species of the genus Pseudoalteromonas (Fig. 1) The predominant isoprenoid quinone detected in strain DB-2 T was ubiquinone-8 (Q-8), which is the same as that shown in some species of the genus Pseudoalteromonas (Zhao et al, 2014). The cellular fatty acid profiles of strain DB-2 T and the type strains of P. haloplanktis, P. mariniglutinosa, P. spongiae and P. tetraodonis are compared in Table 2.…”

“…Phylogenetic analysis based on 16S rRNA gene sequences has shown that the genus Pseudoalteromonas falls within the class Gammaproteobacteria (Gauthier et al, 1995). Members of the genus Pseudoalteromonas have been isolated from a variety of marine environments (Mikhailov et al, 2006;Zhao et al, 2014). During a screening of novel bacteria from a tidal flat at Daebu island in the Yellow Sea, South Korea, many novel bacterial strains have been recently isolated and characterized taxonomically (Park et al, 2016).…”