Pseudokineococcus lusitanus gen. nov., sp. nov., and reclassification of Kineococcus marinus Lee 2006 as Pseudokineococcus marinus comb. nov.

Abstract

A Gram-reaction-positive, motile, coccus-shaped actinobacterium, designated strain T2A-S27T, was isolated from a roof tile in Oporto (Portugal) and studied using a polyphasic approach. The 16S rRNA gene sequence of the novel isolate showed high similarity to that of Kineococcus marinus KST3-3T (97.8 % sequence similarity). Strain T2A-S27T showed lower 16S rRNA gene sequence similarities with other members of the genus Kineococcus and members of the family Kineosporiaceae (<94 %). A phylogenetic tree, based on 16S rRNA gene sequences, showed that strain T2A-S27T formed a coherent clade with the type strain of K. marinus and Quadrisphaera granulorum. The isolate was characterized by the presence of meso-diaminopimelic acid in the cell-wall peptidoglycan, MK-9(H2) as the predominant menaquinone and a polar lipid profile consisting of diphosphatidylglycerol and phosphatidylglycerol. The fatty acid profile was dominated by anteiso-C15 : 0. The DNA G+C content was 76.9 mol%. The low level of DNA–DNA relatedness to K. marinus (46–47 %) and the results of the chemotaxonomic and physiological studies clearly distinguished strain T2A-S27T from recognized species of the genus Kineococcus. On the basis of its phylogenetic position and phenotypic traits, strain T2A-S27T ( = LMG 24148T = CECT 7306T = DSM 23768T) represents a novel species of a new genus in the family Kineosporiaceae, for which the name Pseudokineococcus lusitanus gen. nov., sp. nov. is proposed. The misclassified species K. marinus is transferred to the new genus as Pseudokineococcus marinus comb. nov. The type strain of Pseudokineococcus marinus is KST3-3T ( = KCCM 42250T = NRRL B-24439T).

Draft Genome Sequence of the Gram-Positive Neutrophilic Iron-Precipitating Kineosporia sp. Strain A_224

“…A novel iron-depositing actinobacterial strain of the family Kineosporiaceae was isolated from the floodplain area in the Lower Oder Valley National Park, Germany. Based on 16S rRNA gene sequence analysis, the family Kineosporiaceae ( 1 ) includes the five genera Angustibacter ( 2 ), Kineococcus ( 3 ), Kineosporia ( 4 ), Pseudokineococcus ( 5 ), and Quadrisphaera . The genus Kineosporia contains seven species, K. aurantiaca ( 4 ), K. babensis ( 6 ), K. mesophila ( 7 ), K. mikuniensis , K. rhamnosa , K. rhizophila , and K. succinea ( 8 ).…”

“…Reads were checked for ambiguous base calls and low complexity with the DUST algorithm ( 2 ) and were filtered accordingly with an R script in Microsoft R Open version 3.3.2 ( 3 ), followed by preassembly with SPAdes version 3.10.0 ( 4 ) using default k -mer lengths up to 99 bp. Scaffolds of this preassembly ≥500 bp were subject to extension and second-round scaffolding with SSPACE standard version 3.0 ( 5 ). Scaffolds ≥2,500 bp were assigned to genome bins by MetaBAT version 0.32.4 ( 8 ), and functional annotation of draft genomes was performed with Prokka version 1.12b ( 9 ).…”

Kineococcus gypseus sp. nov., isolated from saline sediment

“…Zhi et al (2009) later proposed the new family Kineosporiaceae comprising the genera Kineococcus , Kineosporia and Quadrisphaera (Maszenan et al , 2005). The genera Angustibacter (Tamura et al , 2010) and Pseudokineococcus (Jurado et al , 2011) were subsequently added to this family. At the time of writing, the genus Kineococcus comprises seven recognized species, namely Kineococcus aurantiacus (Yokota et al , 1993), Kineococcus radiotolerans (Phillips et al , 2002), Kineococcus gynurae (Duangmal et al , 2008), Kineococcus xinjiangensis (Liu et al , 2009), Kineococcus rhizosphaerae (Lee, 2009), Kineococcus glutinatus (Nie et al , 2012; Euzéby, 2012) and Kineococcus endophytica (Bian et al , 2012).…”

“…At the time of writing, the genus Kineococcus comprises seven recognized species, namely Kineococcus aurantiacus (Yokota et al , 1993), Kineococcus radiotolerans (Phillips et al , 2002), Kineococcus gynurae (Duangmal et al , 2008), Kineococcus xinjiangensis (Liu et al , 2009), Kineococcus rhizosphaerae (Lee, 2009), Kineococcus glutinatus (Nie et al , 2012; Euzéby, 2012) and Kineococcus endophytica (Bian et al , 2012). Kineococcus marinus (Lee, 2006) was reclassified within the genus Pseudokineococcus as Pseudokineococcus marinus (Jurado et al , 2011). During a study of the actinomycete diversity of saline–alkaline sediment samples, a Kineococcus -like strain, YIM 121300 T , was isolated and identified by using a polyphasic approach.…”

Angustibacter aerolatus sp. nov., isolated from air

“…Later, two more genera, Angustibacter and Pseudokineococcus, were established by Tamura et al (2010) and Jurado et al (2011), respectively. The genus Angustibacter was named to accommodate one bacterial strain isolated from subarctic forest soil, and, at present, comprises only one species, Angustibacter luteus.…”