Pseudolysogeny of Pseudomonas aeruginosa bacteria infected with φKZ-like bacteriophages

Плетенева, Е. А.; Krylov, S. V.; Shaburova, O. V.; Bourkaltseva, Maria; Miroshnikov, Konstantin A.; Крылов, В. Н.

doi:10.1134/s1022795410010047

Cited by 14 publications

(20 citation statements)

References 16 publications

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“…The mutants have properties of classical virulent phages. Interestingly, phiKZ and EL behave like phages with repressors of different specificity (with phiKZ revealing dominance) [54], see Figure 2. Probably, the substitution of virulent mutants of phiKZ-like phages for wild type phages in therapeutic mixtures decreases the possibility of HGT [55,56].…”

Section: Pseudolysogeny and Pseudovirulencementioning

confidence: 99%

A Genetic Approach to the Development of New Therapeutic Phages to Fight Pseudomonas Aeruginosa in Wound Infections

Крылов

Shaburova

Krylov

et al. 2012

Viruses

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Pseudomonas aeruginosa is a frequent participant in wound infections. Emergence of multiple antibiotic resistant strains has created significant problems in the treatment of infected wounds. Phage therapy (PT) has been proposed as a possible alternative approach. Infected wounds are the perfect place for PT applications, since the basic condition for PT is ensured; namely, the direct contact of bacteria and their viruses. Plenty of virulent (“lytic”) and temperate (“lysogenic”) bacteriophages are known in P. aeruginosa. However, the number of virulent phage species acceptable for PT and their mutability are limited. Besides, there are different deviations in the behavior of virulent (and temperate) phages from their expected canonical models of development. We consider some examples of non-canonical phage-bacterium interactions and the possibility of their use in PT. In addition, some optimal approaches to the development of phage therapy will be discussed from the point of view of a biologist, considering the danger of phage-assisted horizontal gene transfer (HGT), and from the point of view of a surgeon who has accepted the Hippocrates Oath to cure patients by all possible means. It is also time now to discuss the possible approaches in international cooperation for the development of PT. We think it would be advantageous to make phage therapy a kind of personalized medicine.

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Section: Pseudolysogeny and Pseudovirulencementioning

confidence: 99%

A Genetic Approach to the Development of New Therapeutic Phages to Fight Pseudomonas Aeruginosa in Wound Infections

Крылов

Shaburova

Krylov

et al. 2012

Viruses

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“…Bacteriophage 201Φ2-1 is a member of the ΦKZ-like group of viruses, 15 notable due to the size of their genome and coat 16,17 . Although ΦKZ-like bacteriophages are known pseudolysogens, 18 there is no evidence for a role in partitioning. 201Φ2-1 TubZ (PhuZ) is instead thought to localise bacteriophages within the bacterium, forming cytomotive filaments within the host cell that position virions at the cell centre for efficient release upon lysis 6 …”

Section: Introductionmentioning

confidence: 99%

Structure of the Tubulin/FtsZ-Like Protein TubZ from Pseudomonas Bacteriophage ΦKZ

Aylett

Izoré

Amos

et al. 2013

Journal of Molecular Biology

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Pseudomonas ΦKZ-like bacteriophages encode a group of related tubulin/FtsZ-like proteins believed to be essential for the correct centring of replicated bacteriophage virions within the bacterial host. In this study, we present crystal structures of the tubulin/FtsZ-like protein TubZ from Pseudomonas bacteriophage ΦKZ in both the monomeric and protofilament states, revealing that ΦKZ TubZ undergoes structural changes required to polymerise, forming a canonical tubulin/FtsZ-like protofilament. Combining our structures with previous work, we propose a polymerisation–depolymerisation cycle for the Pseudomonas bacteriophage subgroup of tubulin/FtsZ-like proteins. Electron cryo-microscopy of ΦKZ TubZ filaments polymerised in vitro implies a long-pitch helical arrangement for the constituent protofilaments. Intriguingly, this feature is shared by the other known subgroup of bacteriophage tubulin/FtsZ-like proteins from Clostridium species, which are thought to be involved in partitioning the genomes of bacteriophages adopting a pseudo-lysogenic life cycle.

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“…The phiKZ-like group of giant phages infecting P. aeruginosa includes several species and are of interest not only because of their use in a therapeutic setting, but also as a unique model for the study of phage evolution and specific packaging of the genomic DNA ( Burkal’tseva et al, 2002 ; Mesyanzhinov et al, 2002 ; Krylov et al, 2003 , 2004 , 2005 , 2011 ; Hertveldt et al, 2005 ; Shaburova et al, 2008 ; Pleteneva et al, 2010 ; Sokolova et al, 2014 ). This group of Myoviridae phages includes several phage species that exhibit common properties of the type member phiKZ (particle size and morphology, the presence of an inner body in the capsid, and a specific packaging mechanism of phage genomic DNA).…”

Section: Features Of Species Selected For Monospecies Phage Mixtures mentioning

confidence: 99%

Modular Approach to Select Bacteriophages Targeting Pseudomonas aeruginosa for Their Application to Children Suffering With Cystic Fibrosis

et al. 2016

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This review discusses the potential application of bacterial viruses (phage therapy) toward the eradication of antibiotic resistant Pseudomonas aeruginosa in children with cystic fibrosis (CF). In this regard, several potential relationships between bacteria and their bacteriophages are considered. The most important aspect that must be addressed with respect to phage therapy of bacterial infections in the lungs of CF patients is in ensuring the continuity of treatment in light of the continual occurrence of resistant bacteria. This depends on the ability to rapidly select phages exhibiting an enhanced spectrum of lytic activity among several well-studied phage groups of proven safety. We propose a modular based approach, utilizing both mono-species and hetero-species phage mixtures. With an approach involving the visual recognition of characteristics exhibited by phages of well-studied phage groups on lawns of the standard P. aeruginosa PAO1 strain, the simple and rapid enhancement of the lytic spectrum of cocktails is permitted, allowing the development of tailored preparations for patients capable of circumventing problems associated with phage resistant bacterial mutants.

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Pseudolysogeny of Pseudomonas aeruginosa bacteria infected with φKZ-like bacteriophages

Cited by 14 publications

References 16 publications

A Genetic Approach to the Development of New Therapeutic Phages to Fight Pseudomonas Aeruginosa in Wound Infections

A Genetic Approach to the Development of New Therapeutic Phages to Fight Pseudomonas Aeruginosa in Wound Infections

Structure of the Tubulin/FtsZ-Like Protein TubZ from Pseudomonas Bacteriophage ΦKZ

Modular Approach to Select Bacteriophages Targeting Pseudomonas aeruginosa for Their Application to Children Suffering With Cystic Fibrosis

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